| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 28, 21055-21060, July 14, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the B-MYB is a ubiquitously expressed transcription
factor involved in the regulation of cell survival, proliferation, and
differentiation. In an attempt to isolate B-MYB-regulated genes that
may explain the role of B-MYB in cellular processes, representational
difference analysis was performed in neuroblastoma cell lines with
different levels of B-MYB expression. One of the genes, the
mRNA levels of which were enhanced in B-MYB expressing
cells, was ApoJ/ClusterinSGP-2/TRMP-2 (ApoJ/Clusterin),
previously implicated in regulation of apoptosis and tumor
progression. Here we show that the human ApoJ/Clusterin gene contains a
Myb binding site in its 5' flanking region, which interacts with
bacterially synthesized B-MYB protein and mediates B-MYB-dependent transactivation of the ApoJ/Clusterin
promoter in transient transfection assays. Endogenous ApoJ/Clusterin
expression is induced in mammalian cell lines following transient
transfection of a B-MYB cDNA. Blockage of secreted
clusterin by a monoclonal antibody results in increased apoptosis of
neuroblastoma cells exposed to the chemotherapeutic drug doxorubicin.
Thus, activation of ApoJ/Clusterin by B-MYB may be an important step in
the regulation of apoptosis in normal and diseased cells.
Direct Transactivation of the Anti-apoptotic Gene Apolipoprotein
J (Clusterin) by B-MYB*
,,,
,, and**
Laboratory of Molecular Pharmacology and
Pathology, Consorzio Mario Negri Sud, 66030 S. Maria Imbaro, Italy,
the § Section of Toxicology and Biomedical Sciences, ENEA
00060 Rome, Italy, and the Department of
Microbiology/Immunology, Kimmel Cancer Institute, Thomas Jefferson
University, Philadelphia, Pennsylvania 19107
*
This work was supported by grants from the Associazione
Italiana per la Lotta al Neuroblastoma (to G. R. and A. S.), the
Associazione Italiana per la Ricerca sul Cancro (to G. R.), and the
National Institutes of Health (to B. C.). The Fondazione Italiana per
la Ricerca sul Cancro and the Consiglio Nazionale delle Ricerche are
gratefully acknowledged for granting short-term fellowships to A. S.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Ko, L. Shi, S. Kim, C. S. Song, and B. Chatterjee Interplay of Nuclear Factor-{kappa}B and B-myb in the Negative Regulation of Androgen Receptor Expression by Tumor Necrosis Factor {alpha} Mol. Endocrinol., February 1, 2008; 22(2): 273 - 286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Andersen, T. Schepeler, K. Thorsen, K. Birkenkamp-Demtroder, F. Mansilla, L. A. Aaltonen, S. Laurberg, and T. F. Orntoft Clusterin Expression in Normal Mucosa and Colorectal Cancer Mol. Cell. Proteomics, June 1, 2007; 6(6): 1039 - 1048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. GLEAVE and K. N. CHI Knock-down of the Cytoprotective Gene, Clusterin, to Enhance Hormone and Chemosensitivity in Prostate and Other Cancers Ann. N.Y. Acad. Sci., November 1, 2005; 1058(1): 1 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Santilli, R. Schwab, R. Watson, C. Ebert, B. J. Aronow, and A. Sala Temperature-dependent Modification and Activation of B-MYB: IMPLICATIONS FOR CELL SURVIVAL J. Biol. Chem., April 22, 2005; 280(16): 15628 - 15634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Criswell, M. Beman, S. Araki, K. Leskov, E. Cataldo, L. D. Mayo, and D. A. Boothman Delayed Activation of Insulin-like Growth Factor-1 Receptor/Src/MAPK/Egr-1 Signaling Regulates Clusterin Expression, a Pro-survival Factor J. Biol. Chem., April 8, 2005; 280(14): 14212 - 14221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Orlandi, S. Pucci, A. Ciucci, F. Pichiorri, A. Ferlosio, and L. G. Spagnoli Modulation of Clusterin Isoforms Is Associated With All-Trans Retinoic Acid-Induced Proliferative Arrest and Apoptosis of Intimal Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., February 1, 2005; 25(2): 348 - 353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Santilli, B. J. Aronow, and A. Sala Essential Requirement of Apolipoprotein J (Clusterin) Signaling for I{kappa}B Expression and Regulation of NF-{kappa}B Activity J. Biol. Chem., October 3, 2003; 278(40): 38214 - 38219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Rozovskaia, O. Ravid-Amir, S. Tillib, G. Getz, E. Feinstein, H. Agrawal, A. Nagler, E. F. Rappaport, I. Issaeva, Y. Matsuo, et al. Expression profiles of acute lymphoblastic and myeloblastic leukemias with ALL-1 rearrangements PNAS, June 24, 2003; 100(13): 7853 - 7858. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Tanno, A. Negroni, R. Vitali, M. C. Pirozzoli, V. Cesi, C. Mancini, B. Calabretta, and G. Raschella Expression of Insulin-like Growth Factor-binding Protein 5 in Neuroblastoma Cells Is Regulated at the Transcriptional Level by c-Myb and B-Myb via Direct and Indirect Mechanisms J. Biol. Chem., June 21, 2002; 277(26): 23172 - 23180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. R. Johnson, T. K. Johnson, M. Desler, T. A. Luster, T. Nowling, R. E. Lewis, and A. Rizzino Effects of B-Myb on Gene Transcription. PHOSPHORYLATION-DEPENDENT ACTIVITY AND ACETYLATION BY p300 J. Biol. Chem., February 1, 2002; 277(6): 4088 - 4097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Joyce, L. Gelbert, A. Ciaccia, B. DeHoff, and B. W. Grinnell Gene Expression Profile of Antithrombotic Protein C Defines New Mechanisms Modulating Inflammation and Apoptosis J. Biol. Chem., March 30, 2001; 276(14): 11199 - 11203. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
