|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 28, 21210-21217, July 14, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Departments of As sperm prepare for fertilization,
surface Ca2+ channels must open to initiate required,
Ca2+-mediated events. However, the molecular identity and
functional properties of sperm Ca2+ channels remain
uncertain. Here, we use rapid local perfusion and single-cell
photometry to examine the kinetics of calcium responses of mouse sperm
to depolarizing stimuli. The linear rise of intracellular
[Ca2+] evoked by ~10-s applications of an alkaline high
[K+] medium directly reports activity of voltage-gated
Ca2+ channels. Little response occurs if external
Ca2+ is removed or if external or internal pH is elevated
without depolarization. Responses are inhibited 30-40% by 30-100
µM Ni2+ and more completely by 100-300
µM Cd2+. They resist the dihydropyridines
nitrendipine and PN200-110, but 1-10 µM mibefradil
inhibits reversibly. They also resist the venom toxins calciseptine,
CaV2.2 and CaV2.3 (N- and R-type)
Ca2+ Channels in Depolarization-evoked Entry of
Ca2+ into Mouse Sperm*
§,,, and
Physiology and Biophysics and
¶ Pharmacology, University of Washington School of Medicine,
Seattle, Washington 98195-7290
-conotoxin MVIIC, and kurtoxin, but -conotoxin GVIA (5 µM) inhibits ~50%. GVIA also partially blocks transient, low voltage activated Ca2+ currents of
patch-clamped spermatids. Differential sensitivity of sperm responses
to Ni2+ and Cd2+ and partial blockade by GVIA
indicate that depolarization opens at least two types of voltage-gated
Ca2+ channels in epididymal sperm examined prior to
capacitation. Involvement of a previously undetected CaV2.2
(N-type) channel, suggested by the action of GVIA, is substantiated by
immunodetection of Ca2+ channel 
1B subunits
in sperm and sperm extracts. Resistance to dihydropyridines,
calciseptine, MVIIC, and kurtoxin indicates that CaV1,
CaV2.1, and CaV3 (L-, P/Q-, and T-type)
channels contribute little to this evoked response. Partial sensitivity
to 1 µM mibefradil and an enhanced sensitivity of the
GVIA-resistant component of response to Ni2+ suggest
participation of a CaV2.3 (R-type) channel specified by
previously found 1E subunits. Our examination of
depolarization-evoked Ca2+ entry indicates that mature
sperm possess a larger palette of voltage-gated Ca2+
channels than previously thought. Such diversity may permit specific responses to multiple cues encountered on the path to fertilization.
*
This work was supported by the NICHD, National Institutes of
Health through cooperative agreement U54-HD12629 as part of the Specialized Cooperative Centers Program in Reproduction Research, the
W. M. Keck Foundation, the University of Washington Royalty Research
Fund, and Fellowship We 2344/1-1 from the Deutsche
Forschungsgemeinschaft.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Physiology and Biophysics, Box 357290, University of Washington,
Seattle, WA 98195-7290. Tel.: 206-543-6661; Fax: 206-685-0619; E-mail: donner@u.washington.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Xia and D. Ren Egg Coat Proteins Activate Calcium Entry into Mouse Sperm via CATSPER Channels Biol Reprod, June 1, 2009; 80(6): 1092 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Suarez Control of hyperactivation in sperm Hum. Reprod. Update, November 1, 2008; 14(6): 647 - 657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xia, D. Reigada, C. H Mitchell, and D. Ren CATSPER Channel-Mediated Ca2+ Entry into Mouse Sperm Triggers a Tail-to-Head Propagation Biol Reprod, September 1, 2007; 77(3): 551 - 559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Liu, J. Xia, K.-H. Cho, D. E. Clapham, and D. Ren CatSperbeta, a Novel Transmembrane Protein in the CatSper Channel Complex J. Biol. Chem., June 29, 2007; 282(26): 18945 - 18952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-G. Li, X.-F. Ding, A.-H. Liao, X.-B. Kong, and C.-L. Xiong Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility Mol. Hum. Reprod., May 1, 2007; 13(5): 299 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Marquez and S. S. Suarez Bovine Sperm Hyperactivation Is Promoted by Alkaline-Stimulated Ca2+ Influx Biol Reprod, April 1, 2007; 76(4): 660 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Anderson, K. A. Feathergill, D. P. Waller, and L. J. D. Zaneveld SAMMA Induces Premature Human Acrosomal Loss by Ca2+ Signaling Dysregulation J Androl, July 1, 2006; 27(4): 568 - 577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Darszon, J. J Acevedo, B. E Galindo, E. O Hernandez-Gonzalez, T. Nishigaki, C. L Trevino, C. Wood, and C. Beltran Sperm channel diversity and functional multiplicity. Reproduction, June 1, 2006; 131(6): 977 - 988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jimenez-Gonzalez, F. Michelangeli, C.V. Harper, C.L.R. Barratt, and S.J. Publicover Calcium signalling in human spermatozoa: a specialized 'toolkit' of channels, transporters and stores Hum. Reprod. Update, May 1, 2006; 12(3): 253 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Heydecke, D. Meyer, F. Ackermann, B. Wilhelm, T. Gudermann, and I. Boekhoff The Multi PDZ Domain Protein MUPP1 as a Putative Scaffolding Protein for Organizing Signaling Complexes in the Acrosome of Mammalian Spermatozoa J Androl, May 1, 2006; 27(3): 390 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Jin, A. M. O'Doherty, S. Wang, H. Zheng, K. M. Sanders, and W. Yan Catsper3 and Catsper4 Encode Two Cation Channel-Like Proteins Exclusively Expressed in the Testis Biol Reprod, December 1, 2005; 73(6): 1235 - 1242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Di Zhang and M. Gopalakrishnan Sperm Ion Channels: Molecular Targets for the Next Generation of Contraceptive Medicines? J Androl, November 1, 2005; 26(6): 643 - 653. [Full Text] [PDF]
|
|
|
|
|
|
A. E. Carlson, T. A. Quill, R. E. Westenbroek, S. M. Schuh, B. Hille, and D. F. Babcock Identical Phenotypes of CatSper1 and CatSper2 Null Sperm J. Biol. Chem., September 16, 2005; 280(37): 32238 - 32244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ambrosini, G. Zolese, S. Ambrosi, E. Bertoli, F. Mantero, M. Boscaro, and G. Balercia Idiopathic Infertility: Effect of Palmitoylethanolamide (a Homologue of Anandamide) on Hyperactivated Sperm Cell Motility and Ca2+ Influx J Androl, May 1, 2005; 26(3): 429 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Felix Molecular physiology and pathology of Ca2+-conducting channels in the plasma membrane of mammalian sperm Reproduction, March 1, 2005; 129(3): 251 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Fraire-Zamora and M. T. Gonzalez-Martinez Effect of intracellular pH on depolarization-evoked calcium influx in human sperm Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1688 - C1696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Fukuda, K. Yomogida, M. Okabe, and K. Touhara Functional characterization of a mouse testicular olfactory receptor and its role in chemosensing and in regulation of sperm motility J. Cell Sci., November 15, 2004; 117(24): 5835 - 5845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Spehr, K. Schwane, J. A. Riffell, J. Barbour, R. K. Zimmer, E. M. Neuhaus, and H. Hatt Particulate Adenylate Cyclase Plays a Key Role in Human Sperm Olfactory Receptor-mediated Chemotaxis J. Biol. Chem., September 17, 2004; 279(38): 40194 - 40203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Nolan, D. F. Babcock, G. Wennemuth, W. Brown, K. A. Burton, and G. S. McKnight Sperm-specific protein kinase A catalytic subunit C{alpha}2 orchestrates cAMP signaling for male fertility PNAS, September 14, 2004; 101(37): 13483 - 13488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Carlson, R. E. Westenbroek, T. Quill, D. Ren, D. E. Clapham, B. Hille, D. L. Garbers, and D. F. Babcock CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm PNAS, December 9, 2003; 100(25): 14864 - 14868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Quill, S. A. Sugden, K. L. Rossi, L. K. Doolittle, R. E. Hammer, and D. L. Garbers Hyperactivated sperm motility driven by CatSper2 is required for fertilization PNAS, December 9, 2003; 100(25): 14869 - 14874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Turner Tales From the Tail: What Do We Really Know About Sperm Motility? J Androl, November 1, 2003; 24(6): 790 - 803. [Full Text] [PDF]
|
|
|
|
|
|
B. S. Jaiswal and M. Conti Calcium regulation of the soluble adenylyl cyclase expressed in mammalian spermatozoa PNAS, September 16, 2003; 100(19): 10676 - 10681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Wennemuth, D. F. Babcock, and B. Hille Calcium Clearance Mechanisms of Mouse Sperm J. Gen. Physiol., June 30, 2003; 122(1): 115 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Wennemuth, A. E. Carlson, A. J. Harper, and D. F. Babcock Bicarbonate actions on flagellar and Ca2+-channel responses: initial events in sperm activation Development, April 1, 2003; 130(7): 1317 - 1326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Perez-Reyes Molecular Physiology of Low-Voltage-Activated T-type Calcium Channels Physiol Rev, January 1, 2003; 83(1): 117 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Kerr, W. F. Hanna, J. H. Shaper, and W. W. Wright Characterization of Zona Pellucida Glycoprotein 3 (ZP3) and ZP2 Binding Sites on Acrosome-Intact Mouse Sperm Biol Reprod, June 1, 2002; 66(6): 1585 - 1595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Kirkman-Brown, E. L. Punt, C. L. R. Barratt, and S. J. Publicover Zona Pellucida and Progesterone-Induced Ca2+ Signaling and Acrosome Reaction in Human Spermatozoa J Androl, May 1, 2002; 23(3): 306 - 315. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schurmann, S. Koling, S. Jacobs, P. Saftig, S. Krau{beta}, G. Wennemuth, R. Kluge, and H.-G. Joost Reduced Sperm Count and Normal Fertility in Male Mice with Targeted Disruption of the ADP-Ribosylation Factor-Like 4 (Arl4) Gene Mol. Cell. Biol., April 15, 2002; 22(8): 2761 - 2768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jagannathan, E. L. Punt, Y. Gu, C. Arnoult, D. Sakkas, C. L. R. Barratt, and S. J. Publicover Identification and Localization of T-type Voltage-operated Calcium Channel Subunits in Human Male Germ Cells. EXPRESSION OF MULTIPLE ISOFORMS J. Biol. Chem., March 1, 2002; 277(10): 8449 - 8456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Quill, D. Ren, D. E. Clapham, and D. L. Garbers A voltage-gated ion channel expressed specifically in spermatozoa PNAS, October 23, 2001; 98(22): 12527 - 12531. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |