|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 28, 21435-21443, July 14, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Pathology, Yale University School of
Medicine, New Haven, Connecticut 06520
Platelet-endothelial cell adhesion molecule
(PECAM)-1 is a 130-kDa glycoprotein commonly used as an
endothelium-specific marker. Evidence to date suggests that PECAM-1 is
more than just an endothelial cell marker but is intimately involved in
signal transduction pathways. This is mediated in part by
phosphorylation of specific tyrosine residues within the ITAM domain of
PECAM-1 and by recruitment of adapter and signaling molecules. Recently
we demonstrated that PECAM-1/
Platelet-Endothelial Cell Adhesion Molecule-1 (CD31), a
Scaffolding Molecule for Selected Catenin Family Members Whose
Binding Is Mediated by Different Tyrosine and Serine/Threonine
Phosphorylation*
,
-catenin association functions to
regulate -catenin localization and, moreover, to modulate
-catenin tyrosine phosphorylation levels. Here we show that: 1) not
only -catenin, but also 
-catenin is associated with PECAM-1
in vitro and in vivo; 2) PKC enzyme directly
phosphorylates purified PECAM-1; 3) PKC-derived PECAM-1 serine/threonine phosphorylation inversely correlates with -catenin association; 4) PECAM-1 recruits -catenin to cell-cell junctions in
transfected SW480 cells; and 5) -catenin may recruit PECAM-1 into an
insoluble cytoskeletal fraction. These data further support the
concept that PECAM-1 functions as a binder and modulator of catenins and provides a molecular mechanism for previously
reported PECAM-1/cytoskeleton interactions.
*
This work was supported in part by U.S. Public Health
Service Grants R37-HL28373 and PO1-DK38979 (to J. A. M.), a
Charles H. Hood grant (to E. P.), and a Reed Foundation Fellowship (to N. I.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Current address: Insight Ltd., P. O. Box 2128, Rabin Science Park,
Rehovat 76121, Israel.
§
To whom correspondence should be addressed: Dept. of Pathology,
Yale University School of Medicine, 310 Cedar St., P.O. Box 208023, New
Haven, CT 06520-8023. Tel.: 203-785-3763; Fax: 203-785-7303; E-mail:
joseph.madri@yale.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Wu, K. Tworkoski, M. Michaud, and J. A. Madri Bone Marrow Monocyte PECAM-1 Deficiency Elicits Increased Osteoclastogenesis Resulting in Trabecular Bone Loss J. Immunol., March 1, 2009; 182(5): 2672 - 2679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Garnacho, V. Shuvaev, A. Thomas, L. McKenna, J. Sun, M. Koval, S. Albelda, V. Muzykantov, and S. Muro RhoA activation and actin reorganization involved in endothelial CAM-mediated endocytosis of anti-PECAM carriers: critical role for tyrosine 686 in the cytoplasmic tail of PECAM-1 Blood, March 15, 2008; 111(6): 3024 - 3033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wu, T. Welte, M. Michaud, and J. A. Madri PECAM-1: a multifaceted regulator of megakaryocytopoiesis Blood, August 1, 2007; 110(3): 851 - 859. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Biswas, S. Canosa, D. Schoenfeld, J. Schoenfeld, P. Li, L. C. Cheas, J. Zhang, A. Cordova, B. Sumpio, and J. A. Madri PECAM-1 Affects GSK-3{beta}-Mediated {beta}-Catenin Phosphorylation and Degradation Am. J. Pathol., July 1, 2006; 169(1): 314 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Cook-Mills and T. L. Deem Active participation of endothelial cells in inflammation J. Leukoc. Biol., April 1, 2005; 77(4): 487 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. E. Sumpio, S. Yun, A. C. Cordova, M. Haga, J. Zhang, Y. Koh, and J. A. Madri MAPKs (ERK1/2, p38) and AKT Can Be Phosphorylated by Shear Stress Independently of Platelet Endothelial Cell Adhesion Molecule-1 (CD31) in Vascular Endothelial Cells J. Biol. Chem., March 25, 2005; 280(12): 11185 - 11191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Carrithers, S. Tandon, S. Canosa, M. Michaud, D. Graesser, and J. A. Madri Enhanced Susceptibility to Endotoxic Shock and Impaired STAT3 Signaling in CD31-Deficient Mice Am. J. Pathol., January 1, 2005; 166(1): 185 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. O'Brien, G. Cao, A. Makrigiannakis, and H. M. DeLisser Role of immunoreceptor tyrosine-based inhibitory motifs of PECAM-1 in PECAM-1-dependent cell migration Am J Physiol Cell Physiol, October 1, 2004; 287(4): C1103 - C1113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li, C. N. Hahn, M. Parsons, J. Drew, M. A. Vadas, and J. R. Gamble Role of protein kinase C{zeta} in thrombin-induced endothelial permeability changes: inhibition by angiopoietin-1 Blood, September 15, 2004; 104(6): 1716 - 1724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Bazzoni and E. Dejana Endothelial Cell-to-Cell Junctions: Molecular Organization and Role in Vascular Homeostasis Physiol Rev, July 1, 2004; 84(3): 869 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhou, A. Stuart, L. E. Dettin, G. Rodriguez, B. Hoel, and G. I. Gallicano Desmoplakin is required for microvascular tube formation in culture J. Cell Sci., July 1, 2004; 117(15): 3129 - 3140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Zimmerman, J. M. D. T. Nelissen, S. E. van Emst-de Vries, P. H. G. M. Willems, F. de Lange, J. G. Collard, F. N. van Leeuwen, and C. G. Figdor Cytoskeletal restraints regulate homotypic ALCAM-mediated adhesion through PKC{alpha} independently of Rho-like GTPases J. Cell Sci., June 1, 2004; 117(13): 2841 - 2852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gao, W. Sun, M. Christofidou-Solomidou, M. Sawada, D. K. Newman, C. Bergom, S. M. Albelda, S. Matsuyama, and P. J. Newman PECAM-1 functions as a specific and potent inhibitor of mitochondrial-dependent apoptosis Blood, July 1, 2003; 102(1): 169 - 179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Newman and D. K. Newman Signal Transduction Pathways Mediated by PECAM-1: New Roles for an Old Molecule in Platelet and Vascular Cell Biology Arterioscler. Thromb. Vasc. Biol., June 1, 2003; 23(6): 953 - 964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Righi, S. Deaglio, C. Pecchioni, A. Gregorini, A. L. Horenstein, G. Bussolati, A. Sapino, and F. Malavasi Role of CD31/Platelet Endothelial Cell Adhesion Molecule-1 Expression in in Vitro and in Vivo Growth and Differentiation of Human Breast Cancer Cells Am. J. Pathol., April 1, 2003; 162(4): 1163 - 1174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. O'Brien, P. Lim, J. Sun, and S. M. Albelda PECAM-1-dependent neutrophil transmigration is independent of monolayer PECAM-1 signaling or localization Blood, April 1, 2003; 101(7): 2816 - 2825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Solowiej, P. Biswas, D. Graesser, and J. A. Madri Lack of Platelet Endothelial Cell Adhesion Molecule-1 Attenuates Foreign Body Inflammation because of Decreased Angiogenesis Am. J. Pathol., March 1, 2003; 162(3): 953 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Enciso, D. Gratzinger, T. D. Camenisch, S. Canosa, E. Pinter, and J. A. Madri Elevated glucose inhibits VEGF-A-mediated endocardial cushion formation: modulation by PECAM-1 and MMP-2 J. Cell Biol., February 18, 2003; 160(4): 605 - 615. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bhattacharya, N. Sen, M. T. Yiming, R. Patel, K. Parthasarathi, S. Quadri, A. C. Issekutz, and J. Bhattacharya High Tidal Volume Ventilation Induces Proinflammatory Signaling in Rat Lung Endothelium Am. J. Respir. Cell Mol. Biol., February 1, 2003; 28(2): 218 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Osawa, M. Masuda, K.-i. Kusano, and K. Fujiwara Evidence for a role of platelet endothelial cell adhesion molecule-1 in endothelial cell mechanosignal transduction: is it a mechanoresponsive molecule? J. Cell Biol., August 19, 2002; 158(4): 773 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ji, C. D. O'Brien, M. Feldman, Y. Manevich, P. Lim, J. Sun, S. M. Albelda, and M. I. Kotlikoff PECAM-1 (CD31) regulates a hydrogen peroxide-activated nonselective cation channel in endothelial cells J. Cell Biol., April 1, 2002; 157(1): 173 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Pinter, J. Haigh, A. Nagy, and J. A. Madri Hyperglycemia-Induced Vasculopathy in the Murine Conceptus Is Mediated via Reductions of VEGF-A Expression and VEGF Receptor Activation Am. J. Pathol., April 1, 2001; 158(4): 1199 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. ILAN, A. MOHSENIN, L. CHEUNG, and J. A. MADRI PECAM-1 shedding during apoptosis generates a membrane-anchored truncated molecule with unique signaling characteristics FASEB J, February 1, 2001; 15(2): 362 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ji, C. D. O'Brien, M. Feldman, Y. Manevich, P. Lim, J. Sun, S. M. Albelda, and M. I. Kotlikoff PECAM-1 (CD31) regulates a hydrogen peroxide-activated nonselective cation channel in endothelial cells J. Cell Biol., April 1, 2002; 157(1): 173 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Cao, C. D. O'Brien, Z. Zhou, S. M. Sanders, J. N. Greenbaum, A. Makrigiannakis, and H. M. DeLisser Involvement of human PECAM-1 in angiogenesis and in vitro endothelial cell migration Am J Physiol Cell Physiol, May 1, 2002; 282(5): C1181 - C1190. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |