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J. Biol. Chem., Vol. 275, Issue 28, 21678-21687, July 14, 2000

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Maize cap1 Encodes a Novel SERCA-type Calcium-ATPase with a Calmodulin-binding Domain^*

Chalivendra C. Subbaiah^§ and Martin M. Sachs^¶

From the  Department of Crop Sciences, University of Illinois, Urbana and the ^¶ United States Department of Agriculture/Agricultural Research Service, Soybean/Maize Germplasm, Pathology and Genetics Unit, Urbana, Illinois 61801

A cDNA (CAP1) isolated from maize roots shares sequence identity with genes encoding P-type Ca²⁺-ATPases and restores the growth phenotype of yeast mutants defective in Ca²⁺-pumps. CAP1 was transcribed and translated in the yeast mutant. Furthermore, the membrane-integrated product formed a Ca²⁺-dependent phosphorylated intermediate and supported Ca²⁺ transport. Although CAP1 shares greater sequence identity with mammalian "endoplasmic reticulum-type" Ca²⁺-pumps, it differs from these genes by having features of calmodulin (CaM)-regulated Ca²⁺-pumps. CAP1 from yeast microsomes bound CaM, and the CAP1-dependent Ca²⁺ transport in yeast was stimulated by CaM. Peptides from the C terminus of CAP1 bound CaM. Anti-CAP1 antibodies specifically recognized a maize microsomal polypeptide that also bound CaM. A similar polypeptide also formed a Ca²⁺-dependent phosphoenzyme. Our results suggest that cap1 encodes a novel form of CaM-regulated Ca²⁺-ATPase in maize. CAP1 appears to be encoded by one or two genes in maize. CAP1 RNA is induced only during early anoxia, indicating that the Ca²⁺-pump may play an important role in O₂-deprived maize cells.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EMBL Data Bank with accession number(s) AF096871.

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