| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 29, 22037-22047, July 21, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Ha-Ras is modified by isoprenoid on
Cys186 and by reversibly attached palmitates at
Cys181 and Cys184. Ha-Ras loses 90% of its
transforming activity if Cys181 and Cys184 are
changed to serines, implying that palmitates make important contributions to oncogenicity. However, study of dynamic acylation is
hampered by an absence of methods for acutely manipulating Ha-Ras
palmitoylation in living cells. S-nitrosocysteine (SNC) and, to a more modest extent, S-nitrosoglutathione were
found to rapidly increase [3H]palmitate incorporation
into cellular or oncogenic Ha-Ras in NIH 3T3 cells. In contrast, SNC
decreased [3H]palmitate labeling of the transferrin
receptor and caveolin. SNC accelerated loss of
[3H]palmitate from Ha-Ras, implying that SNC stimulated
deacylation and permitted subsequent reacylation of Ha-Ras. SNC also
decreased Ha-Ras GTP binding and inhibited phosphorylation of the
kinases ERK1 and ERK2 in NIH 3T3 cells. Thus, SNC altered two important properties of Ha-Ras activation state and lipidation. These results identify SNC as a new tool for manipulating palmitate turnover on
Ha-Ras and for studying requirements of repalmitoylation and the
relationship between palmitate cycling, membrane localization, and
signaling by Ha-Ras.
S-Nitrosocysteine Increases Palmitate Turnover on
Ha-Ras in NIH 3T3 Cells*
§¶,
Department of Zoology/Genetics and
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa
State University, Ames, Iowa 50011
*
This work was supported in part by funding from the Roy
J. Carver Charitable Trust and by United States Public Health
Service Grant CA51890.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011. Tel.: 515-294-6125; Fax: 515-294-0453; E-mail: jbuss@iastate.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. W. Raines, M. G. Bonini, and S. L. Campbell Nitric oxide cell signaling: S-nitrosation of Ras superfamily GTPases Cardiovasc Res, July 15, 2007; 75(2): 229 - 239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Silvius, P. Bhagatji, R. Leventis, and D. Terrone K-ras4B and Prenylated Proteins Lacking "Second Signals" Associate Dynamically with Cellular Membranes Mol. Biol. Cell, January 1, 2006; 17(1): 192 - 202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Berzat, J. E. Buss, E. J. Chenette, C. A. Weinbaum, A. Shutes, C. J. Der, A. Minden, and A. D. Cox Transforming Activity of the Rho Family GTPase, Wrch-1, a Wnt-regulated Cdc42 Homolog, Is Dependent on a Novel Carboxyl-terminal Palmitoylation Motif J. Biol. Chem., September 23, 2005; 280(38): 33055 - 33065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Goodwin, K. R. Drake, C. Rogers, L. Wright, J. Lippincott-Schwartz, M. R. Philips, and A. K. Kenworthy Depalmitoylated Ras traffics to and from the Golgi complex via a nonvesicular pathway J. Cell Biol., July 18, 2005; 170(2): 261 - 272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Heo and S. L. Campbell Superoxide Anion Radical Modulates the Activity of Ras and Ras-related GTPases by a Radical-based Mechanism Similar to That of Nitric Oxide J. Biol. Chem., April 1, 2005; 280(13): 12438 - 12445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Colicelli Human RAS Superfamily Proteins and Related GTPases Sci. Signal., September 14, 2004; 2004(250): re13 - re13. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. H. M. Lommerse, G. A. Blab, L. Cognet, G. S. Harms, B. E. Snaar-Jagalska, H. P. Spaink, and T. Schmidt Single-Molecule Imaging of the H-Ras Membrane-Anchor Reveals Domains in the Cytoplasmic Leaflet of the Cell Membrane Biophys. J., January 1, 2004; 86(1): 609 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Baker, H. Zheng, J. Walker, J. L. Coloff, and J. E. Buss Distinct Rates of Palmitate Turnover on Membrane-bound Cellular and Oncogenic H-Ras J. Biol. Chem., May 23, 2003; 278(21): 19292 - 19300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-O. Parat and P. L. Fox Palmitoylation of Caveolin-1 in Endothelial Cells Is Post-translational but Irreversible J. Biol. Chem., May 4, 2001; 276(19): 15776 - 15782. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
