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J. Biol. Chem., Vol. 275, Issue 29, 22273-22277, July 21, 2000
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From the The EBNA1 (for Epstein-Barr nuclear antigen 1)
protein of Epstein-Barr virus governs the replication and partitioning
of the viral genomes during latent infection by binding to specific
recognition sites in the viral origin of DNA replication. The crystal
structure of the DNA binding portion of the EBNA1 protein revealed that this region comprises two structural motifs; a core domain, which mediates protein dimerization and is structurally homologous to the DNA
binding domain of the papillomavirus E2 protein, and a flanking domain,
which mediated all the observed sequence-specific contacts. To test the
possibility that the EBNA1 core domain plays a role in
sequence-specific DNA binding not revealed in the crystal structure, we
examined the effects of point mutations in potential hydrogen bond
donors located in an
Two Domains of the Epstein-Barr Virus Origin DNA-binding Protein,
EBNA1, Orchestrate Sequence-specific DNA Binding*
,,,¶
Department of Medical Genetics and
Microbiology, University of Toronto, Toronto, Ontario M5S 1A8 and the
§ Banting and Best Department of Medical Research,
University of Toronto, Toronto, Ontario M5G 1L6, Canada
-helix of the EBNA1 core domain whose
structural homologue in E2 mediates sequence-specific DNA binding. We
show that these mutations severely reduce the affinity of EBNA1 for its
recognition site, and that the core domain, when expressed in the
absence of the flanking domain, has sequence-specific DNA binding
activity. Flanking domain residues were also found to contribute to the
DNA binding activity of EBNA1. Thus, both the core and flanking domains
of EBNA1 play direct roles in DNA recognition.
*
This work was supported by a grant (to L. F. and
A. M. E.) from the National Cancer Institute of Canada, which
receives funds from the Canadian Cancer Society.The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Medical
Genetics and Microbiology, University of Toronto, 1 Kings College Circle, Toronto, Ontario M5S 1A8, Canada. Tel.: 416-946-3501; Fax:
416-978-6885; Email: lori.frappier@utoronto.ca.
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