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J Biol Chem, Vol. 275, Issue 3, 1551-1556, January 21, 2000
From the Free Radical Metabolite Section, Laboratory of
Pharmacology and Chemistry, NIEHS, National Institutes of Health,
Research Triangle Park, North Carolina 27709, the § Department of
Pharmacology and the ¶ Department of Pharmacy, School of Medicine,
The University of Tokushima, Kuramoto, Tokushima 770, Japan
The objective of this study was to elucidate the
origin of the nitric oxide-forming reactions from nitrite in the
presence of the iron-N-methyl-D-glucamine
dithiocarbamate complex ((MGD)2Fe2+). The
(MGD)2Fe2+ complex is commonly used in electron
paramagnetic resonance (EPR) spectroscopic detection of NO both
in vivo and in vitro. Although it is widely
believed that only NO can react with
(MGD)2Fe2+ complex to form the
(MGD)2Fe2+·NO complex, a recent article
reported that the (MGD)2Fe2+ complex can react
not only with NO, but also with nitrite to produce the characteristic
triplet EPR signal of (MGD)2Fe2+·NO
(Hiramoto, K., Tomiyama, S., and Kikugawa, K. (1997) Free Radical
Res. 27, 505-509). However, no detailed reaction mechanisms were
given. Alternatively, nitrite is considered to be a spontaneous NO
donor, especially at acidic pH values (Samouilov, A., Kuppusamy, P.,
and Zweier, J. L. (1998) Arch Biochem. Biophys. 357, 1-7). However, its production of nitric oxide at physiological pH is unclear. In this report, we demonstrate that the
(MGD)2Fe2+ complex and nitrite reacted to form
NO as follows: 1) (MGD)2Fe2·NO complex
was produced at pH 7.4; 2) concomitantly, the
(MGD)3Fe3+ complex, which is the oxidized form
of (MGD)2Fe2+, was formed; 3) the rate of
formation of the (MGD)2Fe2+·NO complex was a
function of the concentration of [Fe2+]2,
[MGD], [H+] and [nitrite].
Nitric Oxide-forming Reaction between the
Iron-N-Methyl-D-glucamine Dithiocarbamate
Complex and Nitrite*
,
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Laboratory of
Pharmacology and Chemistry, NIEHS, National Institutes of Health, F0-02, Research Triangle Park, NC 27709. Tel.: 1-919-541-7573; E-mail:
tsuchiya@niehs.nih.gov.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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