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J Biol Chem, Vol. 275, Issue 3, 1645-1650, January 21, 2000
From the Laboratory for Molecular Biotechnology, Institute of
Animal Biology and Center for Biotechnology UNIL-EPFL, University of
Lausanne, 1015 Lausanne, Switzerland
Efficient initiation of SV40 DNA replication
requires transcription factors that bind auxiliary sequences flanking
the minimally required origin. To evaluate the possibility that
transcription factors may activate SV40 replication by acting on the
chromatin structure of the origin, we used an in vivo
replication system in which we targeted GAL4 fusion proteins to the
minimally required origin. We found that the proline-rich
transcriptional activation domain of nuclear factor I (NF-I), which has
been previously shown to interact with histone H3, specifically
activates replication. Evaluation of a series of deletion and point
mutants of NF-I indicates that the H3-binding domain and the
replication activity coincide perfectly. Assays with other
transcription factors, such as Sp1, confirmed the correlation between
the interaction with H3 and the activation of replication. These
findings imply that transcription factors such as NF-I can activate
SV40 replication via direct interaction with chromatin components,
thereby contributing to the relief of nucleosomal repression at the
SV40 origin.
The Histone-interacting Domain of Nuclear Factor I Activates
Simian Virus 40 DNA Replication in Vivo*
*
This work was supported by the Swiss National Science
Foundation, the Etat de Vaud, and the University of Lausanne
450th Anniversary Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Laboratory of
Molecular Biotechnology, Center for Biotechnology UNIL-EPFL, Department of Chemistry, Swiss Federal Institute of Technology-Lausanne, 1015 Lausanne, Switzerland. E-mail: nicolas.mermod@iba.unil.ch.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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