JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M003807200 on May 8, 2000

J. Biol. Chem., Vol. 275, Issue 30, 22713-22718, July 28, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/30/22713    most recent
M003807200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kudla, G.
Right arrow Articles by Antonsson, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kudla, G.
Right arrow Articles by Antonsson, B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The Destabilization of Lipid Membranes Induced by the C-terminal Fragment of Caspase 8-cleaved Bid Is Inhibited by the N-terminal Fragment*

Grzegorz KudlaDagger , Sylvie MontessuitDagger , Robert EskesDagger §, Catherine Berrier, Jean-Claude MartinouDagger , Alexandre Ghazi, and Bruno AntonssonDagger ||

From the Dagger  Serono Pharmaceutical Research Institute, Serono International S.A., 14, chemin des Aulx, CH-1228 Plan-les Ouates, Geneva, Switzerland and the  Laboratoire des Biomembranes, UMR CNRS 8619, Universite Paris-Sud, 91405 Orsay, France

Bid is a proapoptotic, BH3-domain-only member of the Bcl-2 family. In Fas-induced apoptosis, Bid is activated through cleavage by caspase 8 into a 15.5-kDa C-terminal fragment (tcBid) and a 6.5 kDa N-terminal fragment (tnBid). Following the cleavage, tcBid translocates to the mitochondria and promotes the release of cytochrome c into the cytosol by a mechanism that is not understood. Here we report that recombinant tcBid can act as a membrane destabilizing agent. tcBid induces destabilization and breaking of planar lipid bilayers without appearance of ionic channels; its destabilizing activity is comparable with that of Bax and at least 30-fold higher than that of full-length Bid. Consistently, tcBid, but not full-length Bid, permeabilizes liposomes at physiological pH. The destabilizing effect of tcBid on liposomes and planar bilayers is independent of the BH3 domain. In contrast, mutations in the BH3 domain impair tcBid ability to induce cytochrome c release from mitochondria. The permeabilizing effect of tcBid on planar bilayers, liposomes, and mitochondria can be inhibited by tnBid. In conclusion, our results suggest a dual role for Bid: BH3-independent membrane destabilization and BH3-dependent interaction with other proteins. Moreover, the dissociation of Bid after cleavage by caspase 8 represents an additional step at which apoptosis may be regulated.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Present address: J.W.G. University Frankfurt am Main, 60596 Frankfurt, Germany.

|| To whom correspondence should be addressed. Tel.: 41-22-706-9802; Fax: 41-22-794-6965; E-mail: bruno.antonsson.ch_gva03@serono.com.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
S. W.G. Tait, E. de Vries, C. Maas, A. M. Keller, C. S. D'Santos, and J. Borst
Apoptosis induction by Bid requires unconventional ubiquitination and degradation of its N-terminal fragment
J. Cell Biol., December 31, 2007; 179(7): 1453 - 1466.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Hetz, P.-A. Vitte, A. Bombrun, T. K. Rostovtseva, S. Montessuit, A. Hiver, M. K. Schwarz, D. J. Church, S. J. Korsmeyer, J.-C. Martinou, et al.
Bax Channel Inhibitors Prevent Mitochondrion-mediated Apoptosis and Protect Neurons in a Model of Global Brain Ischemia
J. Biol. Chem., December 30, 2005; 280(52): 42960 - 42970.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. J. Garcia-Saez, M. Coraiola, M. Dalla Serra, I. Mingarro, G. Menestrina, and J. Salgado
Peptides Derived from Apoptotic Bax and Bid Reproduce the Poration Activity of the Parent Full-Length Proteins
Biophys. J., June 1, 2005; 88(6): 3976 - 3990.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. J. Oh, S. Barbuto, N. Meyer, R.-S. Kim, R. J. Collier, and S. J. Korsmeyer
Conformational Changes in BID, a Pro-apoptotic BCL-2 Family Member, upon Membrane Binding: A SITE-DIRECTED SPIN LABELING STUDY
J. Biol. Chem., January 7, 2005; 280(1): 753 - 767.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. De Marchi, S. Campello, I. Szabo, F. Tombola, J.-C. Martinou, and M. Zoratti
Bax Does Not Directly Participate in the Ca2+-induced Permeability Transition of Isolated Mitochondria
J. Biol. Chem., September 3, 2004; 279(36): 37415 - 37422.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Terrones, B. Antonsson, H. Yamaguchi, H.-G. Wang, J. Liu, R. M. Lee, A. Herrmann, and G. Basanez
Lipidic Pore Formation by the Concerted Action of Proapoptotic BAX and tBID
J. Biol. Chem., July 16, 2004; 279(29): 30081 - 30091.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T.-H. Kim, Y. Zhao, W.-X. Ding, J. N. Shin, X. He, Y.-W. Seo, J. Chen, H. Rabinowich, A. A. Amoscato, and X.-M. Yin
Bid-Cardiolipin Interaction at Mitochondrial Contact Site Contributes to Mitochondrial Cristae Reorganization and Cytochrome c Release
Mol. Biol. Cell, July 1, 2004; 15(7): 3061 - 3072.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. K. Rostovtseva, B. Antonsson, M. Suzuki, R. J. Youle, M. Colombini, and S. M. Bezrukov
Bid, but Not Bax, Regulates VDAC Channels
J. Biol. Chem., April 2, 2004; 279(14): 13575 - 13583.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. A. Renshaw, C. E. Dempsey, F. A. Barnes, S. M. Bagstaff, S. K. Dower, C. D. Bingle, and M. K. B. Whyte
Three Novel Bid Proteins Generated by Alternative Splicing of the Human Bid Gene
J. Biol. Chem., January 23, 2004; 279(4): 2846 - 2855.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
A. R. Jazirehi and B. Bonavida
Resveratrol modifies the expression of apoptotic regulatory proteins and sensitizes non-Hodgkin's lymphoma and multiple myeloma cell lines to paclitaxel-induced apoptosis
Mol. Cancer Ther., January 1, 2004; 3(1): 71 - 84.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
R. Sarig, Y. Zaltsman, R. C. Marcellus, R. Flavell, T. W. Mak, and A. Gross
BID-D59A Is a Potent Inducer of Apoptosis in Primary Embryonic Fibroblasts
J. Biol. Chem., March 14, 2003; 278(12): 10707 - 10715.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
D. Arnoult, P. Parone, J.-C. Martinou, B. Antonsson, J. Estaquier, and J. C. Ameisen
Mitochondrial release of apoptosis-inducing factor occurs downstream of cytochrome c release in response to several proapoptotic stimuli
J. Cell Biol., December 23, 2002; 159(6): 923 - 929.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Basanez, J. C. Sharpe, J. Galanis, T. B. Brandt, J. M. Hardwick, and J. Zimmerberg
Bax-type Apoptotic Proteins Porate Pure Lipid Bilayers through a Mechanism Sensitive to Intrinsic Monolayer Curvature
J. Biol. Chem., December 13, 2002; 277(51): 49360 - 49365.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Yamaguchi and H.-G. Wang
Bcl-XL Protects BimEL-induced Bax Conformational Change and Cytochrome c Release Independent of Interacting with Bax or BimEL
J. Biol. Chem., October 25, 2002; 277(44): 41604 - 41612.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. F. Epand, J.-C. Martinou, M. Fornallaz-Mulhauser, D. W. Hughes, and R. M. Epand
The Apoptotic Protein tBid Promotes Leakage by Altering Membrane Curvature
J. Biol. Chem., August 30, 2002; 277(36): 32632 - 32639.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Grinberg, R. Sarig, Y. Zaltsman, D. Frumkin, N. Grammatikakis, E. Reuveny, and A. Gross
tBID Homooligomerizes in the Mitochondrial Membrane to Induce Apoptosis
J. Biol. Chem., March 29, 2002; 277(14): 12237 - 12245.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Madesh, B. Antonsson, S. M. Srinivasula, E. S. Alnemri, and G. Hajnoczky
Rapid Kinetics of tBid-induced Cytochrome c and Smac/DIABLO Release and Mitochondrial Depolarization
J. Biol. Chem., February 8, 2002; 277(7): 5651 - 5659.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. D. Esposti, J. T. Erler, J. A. Hickman, and C. Dive
Bid, a Widely Expressed Proapoptotic Protein of the Bcl-2 Family, Displays Lipid Transfer Activity
Mol. Cell. Biol., November 1, 2001; 21(21): 7268 - 7276.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
G. Basanez and J. Zimmerberg
HIV and Apoptosis: Death and the Mitochondrion
J. Exp. Med., February 20, 2001; 193(4): F11 - F14.
[Full Text] [PDF]

Home page
 J. Cell Biol.Home page
S. Shimizu, Y. Matsuoka, Y. Shinohara, Y. Yoneda, and Y. Tsujimoto
Essential Role of Voltage-dependent Anion Channel in Various Forms of Apoptosis in Mammalian Cells
J. Cell Biol., January 16, 2001; 152(2): 237 - 250.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. Zha, S. Weiler, K. J. Oh, M. C. Wei, and S. J. Korsmeyer
Posttranslational N-Myristoylation of BID as a Molecular Switch for Targeting Mitochondria and Apoptosis
Science, December 1, 2000; 290(5497): 1761 - 1765.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
G. Basanez, J. Zhang, B. N. Chau, G. I. Maksaev, V. A. Frolov, T. A. Brandt, J. Burch, J. M. Hardwick, and J. Zimmerberg
Pro-apoptotic Cleavage Products of Bcl-xL Form Cytochrome c-conducting Pores in Pure Lipid Membranes
J. Biol. Chem., August 10, 2001; 276(33): 31083 - 31091.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. M. Polster, K. W. Kinnally, and G. Fiskum
BH3 Death Domain Peptide Induces Cell Type-selective Mitochondrial Outer Membrane Permeability
J. Biol. Chem., October 5, 2001; 276(41): 37887 - 37894.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.