JBC Transcription and Nuclear Factor Monoclonals

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Originally published In Press as doi:10.1074/jbc.M003411200 on May 23, 2000

J. Biol. Chem., Vol. 275, Issue 30, 23127-23133, July 28, 2000
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The C-terminal alpha  Helix of Tn5 Transposase Is Required for Synaptic Complex Formation*

Mindy Steiniger-White and William S. ReznikoffDagger

From the Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706

An important step in Tn5 transposition requires transposase-transposase homodimerization to form a synaptic complex competent for cleavage of transposon DNA free from the flanking sequence. We demonstrate that the C-terminal helix of Tn5 transposase (residues 458-468 of 476 total amino acids) is required for synaptic complex formation during Tn5 transposition. Specifically, deletion of eight amino acids or more from the C terminus greatly reduces or abolishes synaptic complex formation in vitro. Due to this impaired synaptic complex formation, transposases lacking eight amino acids are also defective in the cleavage step of transposition. Interactions within the synaptic complex dimer interface were investigated by site-directed mutagenesis, and residues required for synaptic complex formation include amino acids comprising the dimer interface in the Tn5 inhibitor x-ray crystal structure dimer. Because the crystal structure dimer was hypothesized to be the inhibitory complex and not a synaptic complex, this result was surprising. Based on these data, models for both in vivo and in vitro synaptic complex formation are presented.

* This research was supported by National Institutes of Health Grant GM50692 and United States Department of Agriculture Hatch Grant 4184.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Madison, WI 53706. Fax: 608-262-3453; E-mail: reznikoff@biochem.wisc.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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