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J. Biol. Chem., Vol. 275, Issue 30, 23267-23272, July 28, 2000
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From the Wadsworth Center, New York State Department of Health and
Department of Biomedical Sciences, State University of New York,
Albany, New York 12201-2002
Variant histones that differ in amino acid
sequence from S-phase histones are widespread in eukaryotes, yet the
structural changes they cause to nucleosomes and how those changes
affect relevant cellular processes have not been determined. H2A.F/Z is
a highly conserved family of H2A variants. H2Av, the H2A.F/Z variant of
Drosophila melanogaster, was localized in polytene chromosomes by indirect immunofluorescence and in diploid chromosomes by chromatin immunoprecipitation. H2Av was widely distributed in the
genome and not limited to sites of active transcription. H2Av was
present in thousands of euchromatic bands and the heterochromatic chromocenter of polytene chromosomes, and the H2Av antibody
precipitated both transcribed and nontranscribed genes as well as
noncoding euchromatic and heterochromatic sequences. The distribution
of H2Av was not uniform. The complex banding pattern of H2Av in
polytene chromosomes did not parallel the concentration of DNA, as did the pattern of immunofluorescence using H2A antibodies, and the density
of H2Av measured by immunoprecipitation varied between different
sequences. Of the sequences assayed, H2Av was least abundant on 1.688 satellite sequences and most abundant on the hsp70 genes.
Finally, transcription caused, to an equivalent extent, both H2Av and
H2A to be less tightly associated with DNA.
Histone H2A.Z Is Widely but Nonrandomly Distributed in
Chromosomes of Drosophila melanogaster*
*
This work was supported by National Institutes of Health
Grant GM53476.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Wadsworth Center, New
York State Dept. of Health and Dept. of Biomedical Sciences, State
University of New York, P. O. Box 22002, Albany, NY 12201-2002 Tel.:
518-473-4201; Fax: 518-474-3181; E-mail: glaser@wadsworth.org.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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