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Originally published In Press as doi:10.1074/jbc.M002312200 on May 22, 2000

J. Biol. Chem., Vol. 275, Issue 31, 23718-23724, August 4, 2000
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7The Yeast mRNA-binding Protein Npl3p Interacts with the Cap-binding Complex*

Elisa C. ShenDagger , Tracy Stage-Zimmermann, Patricia Chui, and Pamela A. Silver§

From the Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and the Dana Farber Cancer Institute, Boston, Massachusetts 02115

A number of RNA-binding proteins are associated with mRNAs in both the nucleus and the cytoplasm. One of these, Npl3p, is a heterogeneous nuclear ribonucleoprotein-like protein with some similarity to SR proteins and is essential for growth in the yeast S. cerevisiae. Temperature-sensitive alleles have defects in the export of mRNA out of the nucleus (1). In this report, we define a genetic relationship between NPL3 and the nonessential genes encoding the subunits of the cap-binding complex (CBP80 and CBP20). Deletion of CBP80 or CBP20 in combination with certain temperature-sensitive npl3 mutant alleles fail to grow and thus display a synthetic lethal relationship. Further evidence of an interaction between Npl3p and the cap-binding complex was revealed by co-immunoprecipitation experiments; Cbp80p and Cbp20p specifically co-precipitate with Npl3p. However, the interaction of Npl3p with Cbp80p depends on both the presence of Cbp20p and RNA. In addition, we show that Cbp80p is capable of shuttling between the nucleus and the cytoplasm in a manner dependent on the ongoing synthesis of RNA. Taken together, these data support a model whereby mRNAs are co-transcriptionally packaged by proteins including Npl3p and cap-binding complex for export out of the nucleus.

* This work was supported by Grants GM19487 (to T. S. Z.) and National Institutes of Health GM57476 (to P. A. S.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Supported in part by a training grant to the Dana-Farber Cancer Institute.

§ To whom correspondence should be addressed: Dana Farber Cancer Institute, 44 Binney St., Boston, MA 02115. Tel.: 617-632-5102; Fax: 617-632-5103; E-mail: pamela_silver@dfci.harvard.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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