| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 31, 23729-23735, August 4, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From Biochemistry and Biophysics, School of Molecular Biosciences,
Washington State University, Pullman, Washington 99164-4660
A-175-base pair fragment containing the
Xenopus borealis somatic 5 S ribosomal RNA gene was used as
a model system to determine the effect of nucleosome assembly on
nucleotide excision repair (NER) of the major UV photoproduct
(cyclobutane pyrimidine dimer (CPD)) in DNA. Xenopus oocyte
nuclear extracts were used to carry out repair in vitro on
reconstituted, positioned 5 S rDNA nucleosomes. Nucleosome structure
strongly inhibits NER at many CPD sites in the 5 S rDNA fragment while
having little effect at a few sites. The time course of CPD removal at
35 different sites indicates that >85% of the CPDs in the naked DNA
fragment have t1/2 values <2 h, whereas <26% of
the t1/2 values in nucleosomes are <2 h, and 15%
are >8 h. Moreover, removal of histone tails from these
mononucleosomes has little effect on the repair rates. Finally,
nucleosome inhibition of repair shows no correlation with the
rotational setting of a 14-nucleotide-long pyrimidine tract located 30 base pairs from the nucleosome dyad. These results suggest that
inhibition of NER by mononucleosomes is not significantly influenced by
the rotational orientation of CPDs on the histone surface, and histone
tails play little (or no) role in this inhibition.
Nucleotide Excision Repair of the 5 S Ribosomal RNA Gene
Assembled into a Nucleosome*
and
*
This study was supported by National Institutes of Health
Grant ES02614 (NIEHS).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Dept. of Molecular and Cellular Biology, Harvard
University, 16 Divinity Ave., Cambridge, MA 02138.
§
To whom correspondence should be addressed. Tel.: 509-335-6853;
Fax: 509-335-9688; E-mail: smerdon@mail.wsu.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. M. Svedruzic, C. Wang, J. V. Kosmoski, and M. J. Smerdon Accommodation and Repair of a UV Photoproduct in DNA at Different Rotational Settings on the Nucleosome Surface J. Biol. Chem., December 2, 2005; 280(48): 40051 - 40057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Adair, Y. Kwon, G. A. Dement, M. J. Smerdon, and R. Reeves Inhibition of Nucleotide Excision Repair by High Mobility Group Protein HMGA1 J. Biol. Chem., September 16, 2005; 280(37): 32184 - 32192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Li and M. J. Smerdon Dissecting Transcription-coupled and Global Genomic Repair in the Chromatin of Yeast GAL1-10 Genes J. Biol. Chem., April 2, 2004; 279(14): 14418 - 14426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Feng, W. Hu, L. A. Chasin, and M.-s. Tang Effects of genomic context and chromatin structure on transcription-coupled and global genomic repair in mammalian cells Nucleic Acids Res., October 15, 2003; 31(20): 5897 - 5906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Gaillard, D. J. Fitzgerald, C. L. Smith, C. L. Peterson, T. J. Richmond, and F. Thoma Chromatin Remodeling Activities Act on UV-damaged Nucleosomes and Modulate DNA Damage Accessibility to Photolyase J. Biol. Chem., May 9, 2003; 278(20): 17655 - 17663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Hu, Z. Feng, L. A. Chasin, and M.-s. Tang Transcription-coupled and Transcription-independent Repair of Cyclobutane Pyrimidine Dimers in the Dihydrofolate Reductase Gene J. Biol. Chem., October 4, 2002; 277(41): 38305 - 38310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Hara and A. Sancar The SWI/SNF Chromatin-Remodeling Factor Stimulates Repair by Human Excision Nuclease in the Mononucleosome Core Particle Mol. Cell. Biol., October 1, 2002; 22(19): 6779 - 6787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Feng, W. Hu, E. Komissarova, A. Pao, M.-C. Hung, G. M. Adair, and M.-s. Tang Transcription-coupled DNA Repair Is Genomic Context-dependent J. Biol. Chem., April 5, 2002; 277(15): 12777 - 12783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. D. Martini, S. Keeney, and M. A. Osley A Role for Histone H2B During Repair of UV-Induced DNA Damage in Saccharomyces cerevisiae Genetics, April 1, 2002; 160(4): 1375 - 1387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. V. Kosmoski, E. J. Ackerman, and M. J. Smerdon DNA repair of a single UV photoproduct in a designed nucleosome PNAS, August 17, 2001; (2001) 181073398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. V. Kosmoski, E. J. Ackerman, and M. J. Smerdon DNA repair of a single UV photoproduct in a designed nucleosome PNAS, August 28, 2001; 98(18): 10113 - 10118. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
