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J. Biol. Chem., Vol. 275, Issue 31, 23861-23868, August 4, 2000
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§,
,
**,
, and

§§
From the The saliva of blood-feeding arthropods contains
an apyrase that facilitates hematophagy by inhibiting the ADP-induced
aggregation of the host platelets. We report here the isolation of a
salivary gland-specific cDNA encoding a secreted protein that
likely represents the Anopheles gambiae apyrase. We
describe also two additional members of the apyrase/5'-nucleotidase
family. The cDNA corresponding to the AgApyL1 gene
encodes a secreted protein that is closely related in sequence to the
apyrase of the yellow fever mosquito, Aedes aegypti, and
whose expression appears enriched in, but not restricted to, female
salivary glands. The AgApyL2 gene was found searching an
A. gambiae data base, and its expression is restricted to
larval stages. We isolated the gene encoding the presumed A. gambiae apyrase (AgApy) and we tested its putative
promoter for the tissue-specific expression of the LacZ
gene from Escherichia coli in transgenic Drosophila
melanogaster. All the transgenic lines analyzed showed a weak but
unambiguous staining of the adult glands, indicating that some of the
salivary gland-specific transcriptional regulatory elements are
conserved between the malaria mosquito and the fruit fly. The
availability of salivary gland-specific promoters may be useful both
for studies on vector-parasite interactions and, potentially, for the
targeted tissue-specific expression of anti-parasite genes in the mosquito.
Istituto di Parassitologia, Istituto
Pasteur-Fondazione Cenci Bolognetti, Università di Roma "La
Sapienza," 00185 Roma, Italy, ¶ Department of Biology, Imperial
College of Science, Technology, and Medicine, London SW7 2AZ, United
Kingdom,
Institute of Molecular Biology and Biotechnology,
Foundation for Research and Technology, 71110 Heraklion, Crete, Greece,
** Department of Biology, University of Crete, 71110 Heraklion, Crete,
Greece, and 
Dipartimento di Genetica, Biologia
Generale e Molecolare, Università di Napoli Federico II,
80134 Napoli, Italy
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ237704, AJ237705, and AJ237706.
§ Supported by a training fellowship of the University of Rome "La Sapienza." §§ Supported by a postdoctoral fellowship of the Istituto Pasteur-Fondazione Cenci Bolognetti and by European Union Return Grant BIO4-CT98-5020. To whom correspondence should be addressed: Istituto di Parassitologia, Università di Roma "La Sapienza," P. le Aldo Moro 5, Box 6, Roma 62, 00185 Roma, Italy. Tel.: 39-06-4991-4900; Fax: 39-06-4991-4644; E-mail: b.Arca@Caspur.it.This article has been cited by other articles:
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B. Arca, F. Lombardo, J. G. Valenzuela, I. M. B. Francischetti, O. Marinotti, M. Coluzzi, and J. M. C. Ribeiro An updated catalogue of salivary gland transcripts in the adult female mosquito, Anopheles gambiae J. Exp. Biol., October 15, 2005; 208(20): 3971 - 3986. [Abstract] [Full Text] [PDF] |
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E. Faudry, S. P. Lozzi, J. M. Santana, M. D'Souza-Ault, S. Kieffer, C. R. Felix, C. A. O. Ricart, M. V. Sousa, T. Vernet, and A. R. L. Teixeira Triatoma infestans Apyrases Belong to the 5'-Nucleotidase Family J. Biol. Chem., May 7, 2004; 279(19): 19607 - 19613. [Abstract] [Full Text] [PDF] |
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