HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 275, Issue 31, 23861-23868, August 4, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/31/23861    most recent M909547199v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lombardo, F. Articles by Arcà, B. Search for Related Content PubMed PubMed Citation Articles by Lombardo, F. Articles by Arcà, B. Social Bookmarking                      What's this?

Promoter Sequences of the Putative Anopheles gambiae Apyrase Confer Salivary Gland Expression in Drosophila melanogaster^*

Fabrizio Lombardo^§, Manlio Di Cristina^¶, Lefteris Spanos, Christos Louis^**, Mario Coluzzi, and Bruno Arcà^§§

From the  Istituto di Parassitologia, Istituto Pasteur-Fondazione Cenci Bolognetti, Università di Roma "La Sapienza," 00185 Roma, Italy, ^¶ Department of Biology, Imperial College of Science, Technology, and Medicine, London SW7 2AZ, United Kingdom,  Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, 71110 Heraklion, Crete, Greece, ^** Department of Biology, University of Crete, 71110 Heraklion, Crete, Greece, and  Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli Federico II, 80134 Napoli, Italy

The saliva of blood-feeding arthropods contains an apyrase that facilitates hematophagy by inhibiting the ADP-induced aggregation of the host platelets. We report here the isolation of a salivary gland-specific cDNA encoding a secreted protein that likely represents the Anopheles gambiae apyrase. We describe also two additional members of the apyrase/5'-nucleotidase family. The cDNA corresponding to the AgApyL1 gene encodes a secreted protein that is closely related in sequence to the apyrase of the yellow fever mosquito, Aedes aegypti, and whose expression appears enriched in, but not restricted to, female salivary glands. The AgApyL2 gene was found searching an A. gambiae data base, and its expression is restricted to larval stages. We isolated the gene encoding the presumed A. gambiae apyrase (AgApy) and we tested its putative promoter for the tissue-specific expression of the LacZ gene from Escherichia coli in transgenic Drosophila melanogaster. All the transgenic lines analyzed showed a weak but unambiguous staining of the adult glands, indicating that some of the salivary gland-specific transcriptional regulatory elements are conserved between the malaria mosquito and the fruit fly. The availability of salivary gland-specific promoters may be useful both for studies on vector-parasite interactions and, potentially, for the targeted tissue-specific expression of anti-parasite genes in the mosquito.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EMBL Data Bank with accession number(s) AJ237704, AJ237705, and AJ237706.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				X.-G. CHEN, O. MARINOTTI, L. WHITMAN, N. JASINSKIENE, and A. A. JAMES THE ANOPHELES GAMBIAE VITELLOGENIN GENE (VGT2) PROMOTER DIRECTS PERSISTENT ACCUMULATION OF A REPORTER GENE PRODUCT IN TRANSGENIC ANOPHELES STEPHENSI FOLLOWING MULTIPLE BLOODMEALS Am J Trop Med Hyg, June 1, 2007; 76(6): 1118 - 1124. [Abstract] [Full Text] [PDF]

				B. Arca, F. Lombardo, J. G. Valenzuela, I. M. B. Francischetti, O. Marinotti, M. Coluzzi, and J. M. C. Ribeiro An updated catalogue of salivary gland transcripts in the adult female mosquito, Anopheles gambiae J. Exp. Biol., October 15, 2005; 208(20): 3971 - 3986. [Abstract] [Full Text] [PDF]

				E. Faudry, S. P. Lozzi, J. M. Santana, M. D'Souza-Ault, S. Kieffer, C. R. Felix, C. A. O. Ricart, M. V. Sousa, T. Vernet, and A. R. L. Teixeira Triatoma infestans Apyrases Belong to the 5'-Nucleotidase Family J. Biol. Chem., May 7, 2004; 279(19): 19607 - 19613. [Abstract] [Full Text] [PDF]