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J. Biol. Chem., Vol. 275, Issue 31, 24146-24155, August 4, 2000

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Multiple S Gene Family Members Including Natural Antisense Transcripts Are Differentially Expressed during Development of Maize Flowers^*

Réjane Ansaldi^§, Annie Chaboud^¶, and Christian Dumas

From the Reproduction et Developpement des Plantes, UMR 5667 CNRS-INRA-ENSL-UCBLyon1, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69634 Lyon Cedex 07, France

Within the large Brassica S gene family, SLG (S locus glycoprotein) and SRK (S locus receptor kinase) participate to the control of pollen-stigma self-incompatibility. In the self-compatible species maize, S gene family members are predominantly expressed in vegetative organs but are also expressed to a lesser extent in the stigma (silk). To determine if the expression of any S gene family members correlates with female receptivity, we analyzed their expression in developing maize silks. We show that a large family of maize S transcripts is expressed in developing silks. Surprisingly, we isolated a cDNA complementary to a large portion of the antisense strand of the maize receptor kinase S domain. Rapid amplification of cDNA ends (RACE)-polymerase chain reaction, RNase protection, and Northern hybridization with single-stranded riboprobes confirmed that natural antisense S transcripts exist in leaves and seedling shoots and in all sexual tissues tested except mature pollen. These natural antisense S transcripts co-exist with several less abundant sense S transcripts. The accumulation of sense and antisense S transcripts is differentially regulated during pollen and silk development. Thus, these results support a role for S gene family members in sexual tissue development and/or compatible pollination and reveal a new level of complexity in the regulation and function of the S gene family in maize.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EMBL Data Bank with accession number(s) AJ001485 and AJ001486.

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