JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M001883200 on May 23, 2000

J. Biol. Chem., Vol. 275, Issue 32, 24375-24382, August 11, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/32/24375    most recent
M001883200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Meininghaus, M.
Right arrow Articles by Eick, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meininghaus, M.
Right arrow Articles by Eick, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Conditional Expression of RNA Polymerase II in Mammalian Cells
DELETION OF THE CARBOXYL-TERMINAL DOMAIN OF THE LARGE SUBUNIT AFFECTS EARLY STEPS IN TRANSCRIPTION*

Mark Meininghaus, Rob D. Chapman, Manuela Horndasch, and Dirk EickDagger

From the Institute for Clinical Molecular Biology and Tumor Genetics, GSF-Research Center for Environment and Health, Marchioninistrasse 25, D-81377 Munich, Germany

The carboxyl-terminal domain (CTD) of the large subunit of mammalian RNA polymerase II contains 52 repeats of a heptapeptide that is the target of a variety of kinases. The hyperphosphorylated CTD recruits important factors for mRNA capping, splicing, and 3'-processing. The role of the CTD for the transcription process in vivo, however, is not yet clear. We have conditionally expressed an alpha -amanitin-resistant large subunit with an almost entirely deleted CTD (LS*Delta 5) in B-cells. These cells have a defect in global transcription of cellular genes in the presence of alpha -amanitin. Moreover, pol II harboring LS*Delta 5 failed to transcribe up to the promoter-proximal pause sites in the hsp70A and c-fos gene promoters. The results indicate that the CTD is already required for steps that occur before promoter-proximal pausing and maturation of mRNA.

* This work was supported by the Deutsche Forschungsgemeinschaft Grant SFB 190 and Fonds der Chemischen Industrie.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 49-89-7099512; Fax: 49-89-7099500; E-mail: eick@gsf.de.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
P. Liu, A. L. Greenleaf, and J. W. Stiller
The Essential Sequence Elements Required for RNAP II Carboxyl-terminal Domain Function in Yeast and Their Evolutionary Conservation
Mol. Biol. Evol., April 1, 2008; 25(4): 719 - 727.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Chang, X. Nie, H. E. Chang, Z. Han, and J. Taylor
Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II
J. Virol., February 1, 2008; 82(3): 1118 - 1127.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
J. E. Perez-Ortin
Genomics of mRNA turnover
Brief Funct Genomic Proteomic, January 22, 2008; (2008) elm029v1.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
R. D. Chapman, M. Heidemann, T. K. Albert, R. Mailhammer, A. Flatley, M. Meisterernst, E. Kremmer, and D. Eick
Transcribing RNA Polymerase II Is Phosphorylated at CTD Residue Serine-7
Science, December 14, 2007; 318(5857): 1780 - 1782.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
N. Custodio, M. Vivo, M. Antoniou, and M. Carmo-Fonseca
Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site
J. Cell Biol., October 22, 2007; 179(2): 199 - 207.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
S. Boireau, P. Maiuri, E. Basyuk, M. de la Mata, A. Knezevich, B. Pradet-Balade, V. Backer, A. Kornblihtt, A. Marcello, and E. Bertrand
The transcriptional cycle of HIV-1 in real-time and live cells
J. Cell Biol., October 22, 2007; 179(2): 291 - 304.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Lux, H. Albiez, R. D. Chapman, M. Heidinger, M. Meininghaus, R. Brack-Werner, A. Lang, M. Ziegler, T. Cremer, and D. Eick
Transition from initiation to promoter proximal pausing requires the CTD of RNA polymerase II
Nucleic Acids Res., September 12, 2005; 33(16): 5139 - 5144.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. D. Chapman, M. Conrad, and D. Eick
Role of the Mammalian RNA Polymerase II C-Terminal Domain (CTD) Nonconsensus Repeats in CTD Stability and Cell Proliferation
Mol. Cell. Biol., September 1, 2005; 25(17): 7665 - 7674.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. Sano and M. D. Schneider
Cyclin-Dependent Kinase-9: An RNAPII Kinase at the Nexus of Cardiac Growth and Death Cascades
Circ. Res., October 29, 2004; 95(9): 867 - 876.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. Y. Jacobs, I. Ogiwara, and A. M. Weiner
Role of the C-Terminal Domain of RNA Polymerase II in U2 snRNA Transcription and 3' Processing
Mol. Cell. Biol., January 15, 2004; 24(2): 846 - 855.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. D. Chapman, B. Palancade, A. Lang, O. Bensaude, and D. Eick
The last CTD repeat of the mammalian RNA polymerase II large subunit is important for its stability
Nucleic Acids Res., January 2, 2004; 32(1): 35 - 44.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Schlosser, M. Holzel, M. Murnseer, H. Burtscher, U. H. Weidle, and D. Eick
A role for c-Myc in the regulation of ribosomal RNA processing
Nucleic Acids Res., November 1, 2003; 31(21): 6148 - 6156.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Fan, X. Yang, W. Wang, W. H. Wood III, K. G. Becker, and M. Gorospe
Global analysis of stress-regulated mRNA turnover by using cDNA arrays
PNAS, August 6, 2002; 99(16): 10611 - 10616.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Lin, R. Taube, K. Fujinaga, and B. M. Peterlin
P-TEFb Containing Cyclin K and Cdk9 Can Activate Transcription via RNA
J. Biol. Chem., May 3, 2002; 277(19): 16873 - 16878.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Chittajallu, Y. Chen, H. Wang, X. Yuan, C. A. Ghiani, T. Heckman, C. J. McBain, and V. Gallo
Regulation of Kv1 subunit expression in oligodendrocyte progenitor cells and their role in G1/S phase progression of the cell cycle
PNAS, February 19, 2002; 99(4): 2350 - 2355.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. Taube, X. Lin, D. Irwin, K. Fujinaga, and B. M. Peterlin
Interaction between P-TEFb and the C-Terminal Domain of RNA Polymerase II Activates Transcriptional Elongation from Sites Upstream or Downstream of Target Genes
Mol. Cell. Biol., January 1, 2002; 22(1): 321 - 331.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Schuhmacher, F. Kohlhuber, M. Holzel, C. Kaiser, H. Burtscher, M. Jarsch, G. W. Bornkamm, G. Laux, A. Polack, U. H. Weidle, et al.
The transcriptional program of a human B cell line in response to Myc
Nucleic Acids Res., January 15, 2001; 29(2): 397 - 406.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Ramanathan, S. M. Rajpara, S. M. Reza, E. Lees, S. Shuman, M. B. Mathews, and T. Pe'ery
Three RNA Polymerase II Carboxyl-terminal Domain Kinases Display Distinct Substrate Preferences
J. Biol. Chem., March 30, 2001; 276(14): 10913 - 10920.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.