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Originally published In Press as doi:10.1074/jbc.M905750199 on May 26, 2000

J. Biol. Chem., Vol. 275, Issue 32, 24993-24999, August 11, 2000
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A Maize Homologue of the Bacterial CMP-3-Deoxy-D-manno-2-octulosonate (KDO) Synthetases
SIMILAR PATHWAYS OPERATE IN PLANTS AND BACTERIA FOR THE ACTIVATION OF KDO PRIOR TO ITS INCORPORATION INTO OUTER CELLULAR ENVELOPES*

Joaquín Royo, Elisa Gómez, and Gregorio HuerosDagger

From the Departamento de Biología Celulare y Genética, Universidad de Alcalá, ES-28871, Alcalá de Henares, Spain

The eight-carbon acid sugar 3-deoxy-D-manno-2-octulosonate (KDO) is an essential component of Gram-negative bacterial cell walls and capsular polysaccharides. KDO is incorporated into these polymers as CMP-KDO, which is produced in an unusual activation step catalyzed by the enzyme CMP-KDO synthetase. CMP-KDO synthetase activity has traditionally been considered exclusive to Gram-negative bacteria. CMP-KDO synthetase inhibitors attract great interest owing to their potential as selective bactericides. The sugar KDO is also a component of the rhamnogalacturonan II pectin fraction of the primary cell walls of most higher plants and of the cell wall polysaccharides of some green algae. However, the metabolic pathway leading to its incorporation into the plant cell wall is unknown. This paper describes the isolation and characterization of a maize gene, which codes for a protein very similar in sequence and activity to prokaryotic CMP-KDO synthetases. Remarkably, the maize gene can complement a CMP-KDO synthetase (kdsB) Salmonella typhimurium mutant defective in cell wall synthesis. ZmCKS activity is novel in eukaryotes. The evolutionary origin of ZmCKS is discussed in relation to the high degree of conservation between the plant and bacterial genes and its atypical codon usage in maize.

* This work was supported by European Community Contract BIO4 CT-97 2158 and a grant from the University of Alcalá.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ242474 and AJ250331.

Dagger To whom correspondence should be addressed: Dpto. Biol. Cel. y Genética, Univ. Alcalá, ES-28871, Alcalá de Henares, Spain. Tel.: 34918854758; Fax: 34918854799; E-mail: gregorio.hueros@alcala.es.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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