|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 33, 25194-25201, August 18, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Molecular Endocrinology Laboratories, Departments of
Cellular and Molecular Physiology and Type II iodothyronine 5'-deiodinase catalyzes the
bioactivation of thyroid hormone in the brain. In astrocytes, this
~200-kDa, membrane-bound enzyme is composed of at least one p29
subunit, an ~60-kDa, cAMP-induced activation protein, and one or more
uniden- tified catalytic subunit(s). Recently, an artificial type
II-like selenodeiodinase was engineered by fusing two independent
cDNAs together; however, no native type II selenodeiodinase
polypeptide is translated in the brain or brown adipose tissue of rats.
These data suggest that the native type II 5'-deiodinase in rat brain is unrelated to this artificial selenoprotein. In this report, we
describe the cloning of the 29-kDa subunit (p29) of type II 5'-deiodinase from a The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF245040.
Cloning, Expression, and Functional Characterization of the
Substrate Binding Subunit of Rat Type II Iodothyronine
5'-Deiodinase*
, and Molecular Genetics
and Microbiology, University of Massachusetts Medical School,
Worcester, Massachusetts 01655
zapII cDNA library prepared from
cAMP-induced astrocytes. The 3.3-kilobase (kb) cDNA encodes an
~30-kDa, 277-amino acid long, hydrophobic protein lacking
selenocysteine. Northern blot analysis showed that a 3.5-kb p29
mRNA was present in tissues showing type II 5'-deiodinase activity
such as brain and cAMP-stimulated astrocytes. Domain-specific, anti-p29
antibodies specifically immunoprecipitated enzyme activity.
Overexpression of exogenous p29 or a green fluorescence protein
(GFP)-tagged p29 fusion protein led to a >100-fold increase in
deiodinating activity in cAMP-stimulated astrocytes, and the increased
activity was specifically immunoprecipitated by anti-GFP antibodies.
Steady-state reaction kinetics of the enzyme in GFP-tagged
p29-expressing astrocytes are identical to those of the native enzyme
in brain. Direct injection of replication-deficient Ad5-p29GFP virus particles into the cerebral
cortex of neonatal rats leads to a ~2-fold increase in brain type II
5'-deiodinating activity. These data show 1) that the 3.3-kb p29
cDNA encodes an essential subunit of rat type II iodothyronine
5'-deiodinase and 2) identify the first non-selenocysteine
containing subunit of the deiodinase family of enzymes.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Gereben, A. M. Zavacki, S. Ribich, B. W. Kim, S. A. Huang, W. S. Simonides, A. Zeold, and A. C. Bianco Cellular and Molecular Basis of Deiodinase-Regulated Thyroid Hormone Signaling Endocr. Rev., December 1, 2008; 29(7): 898 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Trentin Thyroid hormone and astrocyte morphogenesis. J. Endocrinol., May 1, 2006; 189(2): 189 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. del Barco Barrantes, A. Montero-Pedrazuela, A. Guadano-Ferraz, M.-J. Obregon, R. Martinez de Mena, V. Gailus-Durner, H. Fuchs, T. J. Franz, S. Kalaydjiev, M. Klempt, et al. Generation and Characterization of dickkopf3 Mutant Mice. Mol. Cell. Biol., March 1, 2006; 26(6): 2317 - 2326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kohrle, F. Jakob, B. Contempre, and J. E. Dumont Selenium, the Thyroid, and the Endocrine System Endocr. Rev., December 1, 2005; 26(7): 944 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Alkemade, E. C. Friesema, U. A. Unmehopa, B. O. Fabriek, G. G. Kuiper, J. L. Leonard, W. M. Wiersinga, D. F. Swaab, T. J. Visser, and E. Fliers Neuroanatomical Pathways for Thyroid Hormone Feedback in the Human Hypothalamus J. Clin. Endocrinol. Metab., July 1, 2005; 90(7): 4322 - 4334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Curcio-Morelli, B. Gereben, A. M. Zavacki, B. W. Kim, S. Huang, J. W. Harney, P. R. Larsen, and A. C. Bianco In Vivo Dimerization of Types 1, 2, and 3 Iodothyronine Selenodeiodinases Endocrinology, March 1, 2003; 144(3): 937 - 946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Montero-Pedrazuela, J. Bernal, and A. Guadano-Ferraz Divergent Expression of Type 2 Deiodinase and the Putative Thyroxine-Binding Protein p29, in Rat Brain, Suggests that They Are Functionally Unrelated Proteins Endocrinology, March 1, 2003; 144(3): 1045 - 1052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. MALIK and H. HODGSON The relationship between the thyroid gland and the liver QJM, September 1, 2002; 95(9): 559 - 569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. G. J. M. Kuiper, W. Klootwijk, and T. J. Visser Substitution of Cysteine for a Conserved Alanine Residue in the Catalytic Center of Type II Iodothyronine Deiodinase Alters Interaction with Reducing Cofactor Endocrinology, April 1, 2002; 143(4): 1190 - 1198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Bianco, D. Salvatore, B. Gereben, M. J. Berry, and P. R. Larsen Biochemistry, Cellular and Molecular Biology, and Physiological Roles of the Iodothyronine Selenodeiodinases Endocr. Rev., February 1, 2002; 23(1): 38 - 89. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Chanoine, J. Nève, S. Wu, J. Vanderpas, and P. Bourdoux Selenium Decreases Thyroglobulin Concentrations But Does Not Affect the Increased Thyroxine-to-Triiodothyronine Ratio in Children with Congenital Hypothyroidism J. Clin. Endocrinol. Metab., March 1, 2001; 86(3): 1160 - 1163. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Curcio, M. M. A. Baqui, D. Salvatore, B. H. Rihn, S. Mohr, J. W. Harney, P. R. Larsen, and A. C. Bianco The Human Type 2 Iodothyronine Deiodinase Is a Selenoprotein Highly Expressed in a Mesothelioma Cell Line J. Biol. Chem., August 3, 2001; 276(32): 30183 - 30187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Stachelek, T. F. Kowalik, A. P. Farwell, and J. L. Leonard Myosin V Plays an Essential Role in the Thyroid Hormone-dependent Endocytosis of Type II Iodothyronine 5'-Deiodinase J. Biol. Chem., October 6, 2000; 275(41): 31701 - 31707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Stachelek, R. A. Tuft, L. M. Lifschitz, D. M. Leonard, A. P. Farwell, and J. L. Leonard Real-time Visualization of Processive Myosin 5a-mediated Vesicle Movement in Living Astrocytes J. Biol. Chem., September 14, 2001; 276(38): 35652 - 35659. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |