|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 33, 25465-25470, August 18, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Pituitary gonadotropes transduce hormonal input
into cytoplasmic calcium ([Ca2+]cyt)
oscillations that drive rhythmic exocytosis of gonadotropins. Using
Calcium Green-1 and rhod-2 as optical measures of cytoplasmic and
mitochondrial free Ca2+, we show that mitochondria
sequester Ca2+ and tune the frequency of
[Ca2+]cyt oscillations in rat gonadotropes.
Mitochondria accumulated Ca2+ rapidly and in phase with
elevations of [Ca2+]cyt after GnRH
stimulation or membrane depolarization. Inhibiting mitochondrial
Ca2+ uptake by the protonophore CCCP reduced the frequency
of GnRH-induced [Ca2+]cyt oscillations or,
occasionally, stopped them. Much of the Ca2+ that entered
mitochondria is bound by intramitochondrial Ca2+ buffering
systems. The mitochondrial Ca2+ binding ratio may be
dynamic because [Ca2+]mit appeared to reach a
plateau as mitochondrial Ca2+ accumulation continued. Entry
of Ca2+ into mitochondria was associated with a small drop
in the mitochondrial membrane potential. Ca2+ was extruded
from mitochondria more slowly than it entered, and much of this efflux
could be blocked by CGP-37157, a selective inhibitor of mitochondrial
Na+-Ca2+ exchange. Plasma membrane capacitance
changes in response to depolarizing voltage trains were increased when
CCCP was added, showing that mitochondria lower the local
[Ca2+]cyt near sites that trigger exocytosis.
Thus, we demonstrate a central role for mitochondria in a significant
physiological response.
Mitochondria Shape Hormonally Induced Cytoplasmic Calcium
Oscillations and Modulate Exocytosis*
§,,
Department of Physiology and Biophysics,
University of Washington, Seattle, Washington 98195-7290 and the
¶ Department of Physiology, Emory University,
Atlanta, Georgia 30322
*
This work was supported in part by National Institutes of
Health Grant AR17803, by Cooperative Agreement U54 HD12629 (as part of
the Specialized Cooperative Centers Program in Reproductive Research),
and by funds from the the W. M. Keck Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. T. Nguyen, L. E. Garcia-Chacon, J. N. Barrett, E. F. Barrett, and G. David The {Psi}m depolarization that accompanies mitochondrial Ca2+ uptake is greater in mutant SOD1 than in wild-type mouse motor terminals PNAS, February 10, 2009; 106(6): 2007 - 2011. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. Reyes and V. Parpura Mitochondria Modulate Ca2+-Dependent Glutamate Release from Rat Cortical Astrocytes J. Neurosci., September 24, 2008; 28(39): 9682 - 9691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chalmers and J. G. McCarron The mitochondrial membrane potential and Ca2+ oscillations in smooth muscle J. Cell Sci., January 1, 2008; 121(1): 75 - 85. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Franzini-Armstrong ER-Mitochondria Communication. How Privileged? Physiology, August 1, 2007; 22(4): 261 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Camello-Almaraz, P. J. Gomez-Pinilla, M. J. Pozo, and P. J. Camello Mitochondrial reactive oxygen species and Ca2+ signaling Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1082 - C1088. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Jonas BCL-xL Regulates Synaptic Plasticity Mol. Interv., August 1, 2006; 6(4): 208 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Garcia-Chacon, K. T. Nguyen, G. David, and E. F. Barrett Extrusion of Ca2+ from mouse motor terminal mitochondria via a Na+-Ca2+ exchanger increases post-tetanic evoked release J. Physiol., August 1, 2006; 574(3): 663 - 675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zemkova, A. Balik, Y. Jiang, K. Kretschmannova, and S. S. Stojilkovic Roles of Purinergic P2X Receptors as Pacemaking Channels and Modulators of Calcium-Mobilizing Pathway in Pituitary Gonadotrophs Mol. Endocrinol., June 1, 2006; 20(6): 1423 - 1436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Lee and A. Tse Dominant Role of Mitochondria in Calcium Homeostasis of Single Rat Pituitary Corticotropes Endocrinology, November 1, 2005; 146(11): 4985 - 4993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ben-Shlomo, K. A. Wawrowsky, I. Proekt, N. M. Wolkenfeld, S.-G. Ren, J. Taylor, M. D. Culler, and S. Melmed Somatostatin Receptor Type 5 Modulates Somatostatin Receptor Type 2 Regulation of Adrenocorticotropin Secretion J. Biol. Chem., June 24, 2005; 280(25): 24011 - 24021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yang, H. Liu, Z. Hu, H. Zhu, and T. Xu PKC-induced Sensitization of Ca2+-dependent Exocytosis Is Mediated by Reducing the Ca2+ Cooperativity in Pituitary Gonadotropes J. Gen. Physiol., February 28, 2005; 125(3): 327 - 334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Filippin, P. J. Magalhaes, G. Di Benedetto, M. Colella, and T. Pozzan Stable Interactions between Mitochondria and Endoplasmic Reticulum Allow Rapid Accumulation of Calcium in a Subpopulation of Mitochondria J. Biol. Chem., October 3, 2003; 278(40): 39224 - 39234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Jonas, D. Hoit, J. A. Hickman, T. A. Brandt, B. M. Polster, Y. Fannjiang, E. McCarthy, M. K. Montanez, J. M. Hardwick, and L. K. Kaczmarek Modulation of Synaptic Transmission by the BCL-2 Family Protein BCL-xL J. Neurosci., September 10, 2003; 23(23): 8423 - 8431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Kann, R. Kovacs, and U. Heinemann Metabotropic Receptor-Mediated Ca2+ Signaling Elevates Mitochondrial Ca2+ and Stimulates Oxidative Metabolism in Hippocampal Slice Cultures J Neurophysiol, August 1, 2003; 90(2): 613 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Vila, E. F Barrett, and J. N Barrett Stimulation-induced mitochondrial [Ca2+] elevations in mouse motor terminals: comparison of wild-type with SOD1-G93A J. Physiol., June 15, 2003; 549(3): 719 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kito and H. Suzuki Pacemaker frequency is increased by sodium nitroprusside in the guinea pig gastric antrum J. Physiol., January 1, 2003; 546(1): 191 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhu, B. Hille, and T. Xu From the Cover: Sensitization of regulated exocytosis by protein kinase C PNAS, December 24, 2002; 99(26): 17055 - 17059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Parthasarathi, H. Ichimura, S. Quadri, A. Issekutz, and J. Bhattacharya Mitochondrial Reactive Oxygen Species Regulate Spatial Profile of Proinflammatory Responses in Lung Venular Capillaries J. Immunol., December 15, 2002; 169(12): 7078 - 7086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C M Loughrey, K E MacEachern, P Neary, and G L Smith The relationship between intracellular [Ca2+] and Ca2+ wave characteristics in permeabilised cardiomyocytes from the rabbit J. Physiol., September 15, 2002; 543(3): 859 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Billups and I. D. Forsythe Presynaptic Mitochondrial Calcium Sequestration Influences Transmission at Mammalian Central Synapses J. Neurosci., July 15, 2002; 22(14): 5840 - 5847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Medler and E. L. Gleason Mitochondrial Ca2+ Buffering Regulates Synaptic Transmission Between Retinal Amacrine Cells J Neurophysiol, March 1, 2002; 87(3): 1426 - 1439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Villalobos, L. Nunez, P. Chamero, M. T. Alonso, and J. Garcia-Sancho Mitochondrial [Ca2+] Oscillations Driven by Local High [Ca2+] Domains Generated by Spontaneous Electric Activity J. Biol. Chem., October 19, 2001; 276(43): 40293 - 40297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Rowland, N. K. Irby, and G. A. Spirou Specialized Synapse-Associated Structures within the Calyx of Held J. Neurosci., December 15, 2000; 20(24): 9135 - 9144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Hajnoczky, G. Csordas, M. Madesh, and P. Pacher The machinery of local Ca2+ signalling between sarco-endoplasmic reticulum and mitochondria J. Physiol., November 15, 2000; 529(1): 69 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Collins, P. Lipp, M. J. Berridge, and M. D. Bootman Mitochondrial Ca2+ Uptake Depends on the Spatial and Temporal Profile of Cytosolic Ca2+ Signals J. Biol. Chem., July 6, 2001; 276(28): 26411 - 26420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Arnaudeau, W. L. Kelley, J. V. Walsh Jr., and N. Demaurex Mitochondria Recycle Ca2+ to the Endoplasmic Reticulum and Prevent the Depletion of Neighboring Endoplasmic Reticulum Regions J. Biol. Chem., July 27, 2001; 276(31): 29430 - 29439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Van Goor, D. Zivadinovic, A. J. Martinez-Fuentes, and S. S. Stojilkovic Dependence of Pituitary Hormone Secretion on the Pattern of Spontaneus Voltage-gated Calcium Influx. CELL TYPE-SPECIFIC ACTION POTENTIAL SECRETION COUPLING J. Biol. Chem., August 31, 2001; 276(36): 33840 - 33846. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Johnson, C. J. H. Wong, W. K. Yunker, and J. P. Chang Caffeine-stimulated GTH-II release involves Ca2+ stores with novel properties Am J Physiol Cell Physiol, March 1, 2002; 282(3): C635 - C645. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |