|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 33, 25641-25651, August 18, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the In mammals, the Rh family includes the variable
Rh polypeptides and invariant RhAG glycoprotein. These polytopic
proteins are confined to the erythroid lineage and are assembled into a multisubunit complex essential for Rh antigen expression and plasma membrane integrity. Here, we report the characterization of RhCG and
Rhcg, a pair of novel Rh homologues present in human and mouse nonerythroid tissues. Despite sharing a notable similarity to the
erythroid forms, including the 12-transmembrane topological fold, the
RHCG/Rhcg pair is distinct in chromosome location, genomic organization, promoter structure, and tissue-specific expression. RHCG and Rhcg map at 15q25 of human chromosome
15 and the long arm of mouse chromosome 7, respectively, each having 11 exons and a CpG-rich promoter. Northern blots detected kidney and
testis as the major organs of RHCG or Rhcg
expression. In situ hybridization revealed strong
expression of Rhcg in the kidney collecting tubules and
testis seminiferous tubules. Confocal imaging of transiently expressed
green fluorescence protein fusion proteins localized RhCG
exclusively to the plasma membrane, a distribution confirmed by
cellular fractionation and Western blot analysis. In vitro translation and ex vivo expression showed that RhCG carries
a complex N-glycan, probably at the
48NLS50 sequon of exoloop 1. These results
pinpoint RhCG and Rhcg as novel polytopic membrane glycoproteins that
may function as epithelial transporters maintaining normal homeostatic
conditions in kidney and testis.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF193807, AF193808, AF193809, AF193810, AF183390, AF183391,
AF193811, AF193812, AF209468, AF219981 to AF219986, and AF238372 to
AF238377.
Characterization of Human RhCG and Mouse Rhcg as Novel
Nonerythroid Rh Glycoprotein Homologues Predominantly Expressed in
Kidney and Testis*
,,
Laboratory of Biochemistry and Molecular
Genetics, Lindsley F. Kimball Research Institute, New York Blood
Center, New York, New York 10021, the § Program in
Developmental Biology, The Hospital for Sick Children and Department of
Molecular and Medical Genetics, University of Toronto,
Toronto, Ontario M5G 1X8, Canada, and the ¶ Life Sciences
Division, Lawrence Berkeley National Laboratory,
Berkeley, California 94720
*
This work was supported in part by National Institutes of
Health Grant HL54459 (to C.-H. H.).The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Laboratory of
Biochemistry and Molecular Genetics, Lindsley F. Kimball Research Inst., New York Blood Center, 310 East 67th St., New York, NY 10021.
Tel: 212-570-3388; Fax: 212-570-3251; E-mail: chuang@nybc.org.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K.-H. Han, K. Mekala, V. Babida, H.-Y. Kim, M. E. Handlogten, J. W. Verlander, and I. D. Weiner Expression of the gas-transporting proteins, Rh B glycoprotein and Rh C glycoprotein, in the murine lung Am J Physiol Lung Cell Mol Physiol, July 1, 2009; 297(1): L153 - L163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Weihrauch, M. P. Wilkie, and P. J. Walsh Ammonia and urea transporters in gills of fish and aquatic crustaceans J. Exp. Biol., June 1, 2009; 212(11): 1716 - 1730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-W. Lee, J. W. Verlander, J. M. Bishop, P. Igarashi, M. E. Handlogten, and I. D. Weiner Collecting duct-specific Rh C glycoprotein deletion alters basal and acidosis-stimulated renal ammonia excretion Am J Physiol Renal Physiol, June 1, 2009; 296(6): F1364 - F1375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. N. Brown, D. Hallouane, W. J. Mawby, F. E. Karet, M. A. Saleem, A. J. Howie, and A. M. Toye RhCG is the major putative ammonia transporter expressed in the human kidney, and RhBG is not expressed at detectable levels Am J Physiol Renal Physiol, June 1, 2009; 296(6): F1279 - F1290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. K. N. Tsui, C. Y. C. Hung, C. M. Nawata, J. M. Wilson, P. A. Wright, and C. M. Wood Ammonia transport in cultured gill epithelium of freshwater rainbow trout: the importance of Rhesus glycoproteins and the presence of an apical Na+/NH4+ exchange complex J. Exp. Biol., March 15, 2009; 212(6): 878 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Kim, J. W. Verlander, J. M. Bishop, B. D. Cain, K.-H. Han, P. Igarashi, H.-W. Lee, M. E. Handlogten, and I. D. Weiner Basolateral expression of the ammonia transporter family member Rh C glycoprotein in the mouse kidney Am J Physiol Renal Physiol, March 1, 2009; 296(3): F543 - F555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yoshihara, K. Inoue, D. Schichnes, S. Ruzin, W. Inwood, and S. Kustu An Rh1-GFP Fusion Protein Is in the Cytoplasmic Membrane of a White Mutant Strain of Chlamydomonas reinhardtii Mol Plant, November 14, 2008; (2008) ssn074v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Feller, A. M. Simpson, M. Nelson, M. A. Swan, P. J. O'Connell, W. J. Hawthorne, C. Tao, and B. A. O'Brien Growth-Promoting Effect of Rh(D) Antibody on Human Pancreatic Islet Cells J. Clin. Endocrinol. Metab., September 1, 2008; 93(9): 3560 - 3567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Nawata, C. C. Y. Hung, T. K. N. Tsui, J. M. Wilson, P. A. Wright, and C. M. Wood Ammonia excretion in rainbow trout (Oncorhynchus mykiss): evidence for Rh glycoprotein and H+-ATPase involvement Physiol Genomics, November 14, 2007; 31(3): 463 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. C. Hung, K. N. T. Tsui, J. M. Wilson, C. M. Nawata, C. M. Wood, and P. A. Wright Rhesus glycoprotein gene expression in the mangrove killifish Kryptolebias marmoratus exposed to elevated environmental ammonia levels and air J. Exp. Biol., July 15, 2007; 210(14): 2419 - 2429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Khademi and R. M. Stroud The Amt/MEP/Rh Family: Structure of AmtB and the Mechanism of Ammonia Gas Conduction. Physiology, December 1, 2006; 21(6): 419 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-H. Han, B. P. Croker, W. L. Clapp, D. Werner, M. Sahni, J. Kim, H.-Y. Kim, M. E. Handlogten, and I. D. Weiner Expression of the Ammonia Transporter, Rh C Glycoprotein, in Normal and Neoplastic Human Kidney J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2670 - 2679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yang, D. Zhao, E. Solenov, and A. S. Verkman Evidence from knockout mice against physiologically significant aquaporin 8-facilitated ammonia transport Am J Physiol Cell Physiol, September 1, 2006; 291(3): C417 - C423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Ji, S. Hashmi, Z. Liu, J. Zhang, Y. Chen, and C.-H. Huang CeRh1 (rhr-1) is a dominant Rhesus gene essential for embryonic development and hypodermal function in Caenorhabditis elegans PNAS, April 11, 2006; 103(15): 5881 - 5886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-O. D. Mak, B. Dang, I. D. Weiner, J. K. Foskett, and C. M. Westhoff Characterization of ammonia transport by the kidney Rh glycoproteins RhBG and RhCG Am J Physiol Renal Physiol, February 1, 2006; 290(2): F297 - F305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Seshadri, J. D. Klein, S. Kozlowski, J. M. Sands, Y.-H. Kim, K.-H. Han, M. E. Handlogten, J. W. Verlander, and I. D. Weiner Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis Am J Physiol Renal Physiol, February 1, 2006; 290(2): F397 - F408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Huang and J. Peng Evolutionary conservation and diversification of Rh family genes and proteins PNAS, October 25, 2005; 102(43): 15512 - 15517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Handlogten, S.-P. Hong, L. Zhang, A. W. Vander, M. L. Steinbaum, M. Campbell-Thompson, and I. D. Weiner Expression of the ammonia transporter proteins Rh B glycoprotein and Rh C glycoprotein in the intestinal tract Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G1036 - G1047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lopez, S. Metral, D. Eladari, S. Drevensek, P. Gane, R. Chambrey, V. Bennett, J.-P. Cartron, C. Le Van Kim, and Y. Colin The Ammonium Transporter RhBG: REQUIREMENT OF A TYROSINE-BASED SIGNAL AND ANKYRIN-G FOR BASOLATERAL TARGETING AND MEMBRANE ANCHORAGE IN POLARIZED KIDNEY EPITHELIAL CELLS J. Biol. Chem., March 4, 2005; 280(9): 8221 - 8228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Nakhoul, H. DeJong, S. M. Abdulnour-Nakhoul, E. L. Boulpaep, K. Hering-Smith, and L. L. Hamm Characteristics of renal Rhbg as an NH4+ transporter Am J Physiol Renal Physiol, January 1, 2005; 288(1): F170 - F181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zheng, D. Kostrewa, S. Berneche, F. K. Winkler, and X.-D. Li The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli PNAS, December 7, 2004; 101(49): 17090 - 17095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Ripoche, O. Bertrand, P. Gane, C. Birkenmeier, Y. Colin, and J.-P. Cartron Human Rhesus-associated glycoprotein mediates facilitated transport of NH3 into red blood cells PNAS, December 7, 2004; 101(49): 17222 - 17227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Wagner, K. E. Finberg, S. Breton, V. Marshansky, D. Brown, and J. P. Geibel Renal Vacuolar H+-ATPase Physiol Rev, October 1, 2004; 84(4): 1263 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Handlogten, S.-P. Hong, C. M. Westhoff, and I. D. Weiner Basolateral ammonium transport by the mouse inner medullary collecting duct cell (mIMCD-3) Am J Physiol Renal Physiol, October 1, 2004; 287(4): F628 - F638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cheval, J. P. Duong Van Huyen, P. Bruneval, J.-M. Verbavatz, J.-M. Elalouf, and A. Doucet Plasticity of mouse renal collecting duct in response to potassium depletion Physiol Genomics, September 16, 2004; 19(1): 61 - 73. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Ludewig Electroneutral ammonium transport by basolateral rhesus B glycoprotein J. Physiol., September 15, 2004; 559(3): 751 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. K. Johansson, J. Brodd, C. Eklof, M. Ferletta, G. Hesselager, C.-F. Tiger, L. Uhrbom, and B. Westermark Identification of candidate cancer-causing genes in mouse brain tumors by retroviral tagging PNAS, August 3, 2004; 101(31): 11334 - 11337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Westhoff, D. L. Siegel, C. G. Burd, and J. K. Foskett Mechanism of Genetic Complementation of Ammonium Transport in Yeast by Human Erythrocyte Rh-associated Glycoprotein J. Biol. Chem., April 23, 2004; 279(17): 17443 - 17448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Bakouh, F. Benjelloun, P. Hulin, F. Brouillard, A. Edelman, B. Cherif-Zahar, and G. Planelles NH3 Is Involved in the NH4 Transport Induced by the Functional Expression of the Human Rh C Glycoprotein J. Biol. Chem., April 16, 2004; 279(16): 15975 - 15983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Quentin, D. Eladari, L. Cheval, C. Lopez, D. Goossens, Y. Colin, J.-P. Cartron, M. Paillard, and R. Chambrey RhBG and RhCG, the Putative Ammonia Transporters, Are Expressed in the Same Cells in the Distal Nephron J. Am. Soc. Nephrol., March 1, 2003; 14(3): 545 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Verlander, R. T. Miller, A. E. Frank, I. E. Royaux, Y.-H. Kim, and I. D. Weiner Localization of the ammonium transporter proteins RhBG and RhCG in mouse kidney Am J Physiol Renal Physiol, February 1, 2003; 284(2): F323 - F337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Eladari, L. Cheval, F. Quentin, O. Bertrand, I. Mouro, B. Cherif-Zahar, J.-P. Cartron, M. Paillard, A. Doucet, and R. Chambrey Expression of RhCG, a New Putative NH3/NH4+ Transporter, along the Rat Nephron J. Am. Soc. Nephrol., August 1, 2002; 13(8): 1999 - 2008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Soupene, N. King, E. Feild, P. Liu, K. K. Niyogi, C.-H. Huang, and S. Kustu Rhesus expression in a green alga is regulated by CO2 PNAS, May 28, 2002; 99(11): 7769 - 7773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Westhoff, M. Ferreri-Jacobia, D.-O. D. Mak, and J. K. Foskett Identification of the Erythrocyte Rh Blood Group Glycoprotein as a Mammalian Ammonium Transporter J. Biol. Chem., April 5, 2002; 277(15): 12499 - 12502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Liu and S. E. Wilson Characterization of Human and Mouse Angiopoietin-like Factor CDT6 Promoters Invest. Ophthalmol. Vis. Sci., November 1, 2001; 42(12): 2776 - 2783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Soupene, R. M. Ramirez, and S. Kustu Evidence that Fungal MEP Proteins Mediate Diffusion of the Uncharged Species NH3 across the Cytoplasmic Membrane Mol. Cell. Biol., September 1, 2001; 21(17): 5733 - 5741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu, J. Peng, R. Mo, C.-c. Hui, and C.-H. Huang Rh Type B Glycoprotein Is a New Member of the Rh Superfamily and a Putative Ammonia Transporter in Mammals J. Biol. Chem., January 5, 2001; 276(2): 1424 - 1433. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |