Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M910007199 on June 9, 2000

J. Biol. Chem., Vol. 275, Issue 34, 26507-26514, August 25, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/34/26507    most recent
M910007199v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Borok, Z.
Right arrow Articles by Crandall, E. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Borok, Z.
Right arrow Articles by Crandall, E. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Differential Regulation of Rat Aquaporin-5 Promoter/Enhancer Activities in Lung and Salivary Epithelial Cells*

Zea BorokDagger , Xian Li, Valentino F. J. Fernandes, Beiyun Zhou, David K. Ann§, and Edward D. Crandall

From the Division of Pulmonary and Critical Care Medicine, Department of Medicine and §  Department of Molecular Pharmacology and Toxicology, Will Rogers Institute Pulmonary Research Center and the  Schools of Medicine and Pharmacy, University of Southern California, Los Angeles, California 90033

Aquaporin-5 (AQP5) is a water channel protein that is selectively expressed in respiratory, salivary, and lacrimal tissues. In order to establish the tissue-specific transcriptional programs that underlie its lung- and salivary-specific expression, a 4.5-kilobase pair DNA fragment encompassing the 5'-flanking region of the rat AQP5 gene has been characterized in detail. A major transcription start site utilized in lung and salivary glands has been localized downstream of a TATAA-like motif. Transient transfection assays of -4.3- and -1.7-AQP5-luciferase constructs in AQP5-expressing lung (MLE-15) and salivary (Pa-4) cells and nonexpressing fibroblast (NIH3T3) and epithelial (HeLa) cells demonstrate preferential transcriptional enhancement of reporter activities in MLE-15 and Pa-4 cells. Transient transfection assays of a series of 5' right-arrow 3' deletion constructs of -4.3-AQP5-luciferase suggest that a common salivary and lung enhancer is located between nucleotides -274 and -139, and a lung-specific enhancer is located between nucleotides -894 and -710. There is one putative lung-specific repressor located in the region of nucleotides -1003/-894 and a common lung and salivary repressor located at nucleotides -503/-385. Moreover, 3' right-arrow 5' deletions up to -171 and -127 base pairs almost abolish transcriptional activation in salivary and lung cells, respectively. Together, our findings indicate that the combination of enhancer/repressor elements within the proximal 5'-flanking region of rat AQP5 gene dictates its restricted expression in both lung and salivary cells.

* This work was supported in part by the American Heart Association (to Z. B.), National Institutes of Health Research Grants DE 10742 (to D. K. A.), HL38578 and HL38621 (to E. D. C.), the Baxter Foundation (to Z. B.) and the Hastings Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Division of Pulmonary and Critical Care Medicine, University of Southern California, GNH 11-900, 2025 Zonal Ave., Los Angeles, CA 90033. Tel.: 323-442-3329; Fax: 323-442-2611; E-mail: zborok@hsc.usc.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
B. Zhou, T. A. Francis, H. Yang, W. Tseng, Q. Zhong, B. Frenkel, Edward. E. Morrisey, David. K. Ann, P. Minoo, E. D. Crandall, et al.
GATA-6 mediates transcriptional activation of aquaporin-5 through interactions with Sp1
Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1141 - C1150.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. Zhou, D. K. Ann, P. Flodby, P. Minoo, J. M. Liebler, E. D. Crandall, and Z. Borok
Rat aquaporin-5 4.3-kb 5'-flanking region differentially regulates expression in salivary gland and lung in vivo
Am J Physiol Cell Physiol, July 1, 2008; 295(1): C111 - C120.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Minoo, L. Hu, N. Zhu, Z. Borok, S. Bellusci, J. Groffen, D. Kardassis, and C. Li
SMAD3 prevents binding of NKX2.1 and FOXA1 to the SpB promoter through its MH1 and MH2 domains
Nucleic Acids Res., January 17, 2008; 36(1): 179 - 188.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. Zhou, D. K. Ann, X. Li, K.-J. Kim, H. Lin, P. Minoo, E. D. Crandall, and Z. Borok
Hypertonic induction of aquaporin-5: novel role of hypoxia-inducible factor-1{alpha}
Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1280 - C1290.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. Minoo, L. Hu, Y. Xing, N. L. Zhu, H. Chen, M. Li, Z. Borok, and C. Li
Physical and Functional Interactions between Homeodomain NKX2.1 and Winged Helix/Forkhead FOXA1 in Lung Epithelial Cells
Mol. Cell. Biol., March 15, 2007; 27(6): 2155 - 2165.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
C. Muller, M. Sendler, and J.-P. Hildebrandt
Downregulation of aquaporins 1 and 5 in nasal gland by osmotic stress in ducklings, Anas platyrhynchos: implications for the production of hypertonic fluid
J. Exp. Biol., October 15, 2006; 209(20): 4067 - 4076.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. P. Mendez, S. B. Morris, S. Wilcoxen, E. Greeson, B. Moore, and R. Paine III
Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury
Am J Physiol Lung Cell Mol Physiol, May 1, 2006; 290(5): L962 - L970.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Wang, F. Gigliotti, S. Maggirwar, C. Johnston, J. N. Finkelstein, and T. W. Wright
Pneumocystis carinii Activates the NF-{kappa}B Signaling Pathway in Alveolar Epithelial Cells
Infect. Immun., May 1, 2005; 73(5): 2766 - 2777.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. Brochiero, A. Dagenais, A. Prive, Y. Berthiaume, and R. Grygorczyk
Evidence of a functional CFTR Cl- channel in adult alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L382 - L392.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K.-J. Kim and A. B. Malik
Protein transport across the lung epithelial barrier
Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L247 - L259.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Z. Borok and A. S. Verkman
Lung Edema Clearance: 20 Years of Progress: Invited Review: Role of aquaporin water channels in fluid transport in lung and airways
J Appl Physiol, December 1, 2002; 93(6): 2199 - 2206.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
Y. Song, N. Sonawane, and A S Verkman
Localization of aquaporin-5 in sweat glands and functional analysis using knockout mice
J. Physiol., June 1, 2002; 541(2): 561 - 568.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Z. Borok, J. M. Liebler, R. L. Lubman, M. J. Foster, B. Zhou, X. Li, S. M. Zabski, K.-J. Kim, and E. D. Crandall
Alveolar Epithelial Ion and Fluid Transport: Na transport proteins are expressed by rat alveolar epithelial type I cells
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L599 - L608.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement