JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M002770200 on June 14, 2000

J. Biol. Chem., Vol. 275, Issue 35, 26792-26798, September 1, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/35/26792    most recent
M002770200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Humphreys, B. D.
Right arrow Articles by Dubyak, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Humphreys, B. D.
Right arrow Articles by Dubyak, G. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Stress-activated Protein Kinase/JNK Activation and Apoptotic Induction by the Macrophage P2X7 Nucleotide Receptor*

Benjamin D. HumphreysDagger , Janet Rice, Sylvia B. Kertesy, and George R. Dubyak§

From the Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106

In human and rodent macrophages, activation of the P2X7 nucleotide receptor stimulates interleukin-1beta processing and release, apoptosis, and killing of intracellular Mycobacterium tuberculosis. Signaling pathways downstream of this ionotropic ATP receptor are poorly understood. Here we describe the rapid activation of the stress-activated protein kinase (SAPK)/JNK pathway in BAC1 murine macrophages stimulated by extracellular ATP. Brief exposure of the cells to ATP (10-30 min) was sufficient to trigger a rapid accumulation of activated SAPK that was then sustained for >120 min. Several observations indicated that the P2X7 receptor mediated this effect. 1) ATP and 3'-O-(4-benzoyl)benzoyl-ATP were the only agonistic nucleotides. 2) The effect was inhibited by oxidized ATP and the isoquinoline KN-62, two known P2X7 receptor antagonists. 3) ATP-induced SAPK activation could be recapitulated in P2X7 receptor-transfected HEK293 cells, but not in wild-type HEK293 cells. Because P2X7 receptor stimulation can rapidly activate caspase family proteases that have been implicated in the induction of the SAPK pathway, we investigated whether ATP-dependent SAPK activation involved such proteases. Brief exposure of BAC1 macrophages to extracellular ATP induced DNA fragmentation, alpha -fodrin breakdown, and elevated levels of caspase-3-type activity. Asp-Glu-Val-Asp-cho, a caspase-3 inhibitor, inhibited ATP-induced DNA fragmentation and alpha -fodrin proteolysis, but had no effect on ATP-induced SAPK activation. Tyr-Val-Ala-Asp-chloromethyl ketone, a caspase-1 inhibitor, prevented ATP-induced release of processed interleukin-1beta , but not ATP-dependent SAPK activity. We conclude that activation of ionotropic P2X7 nucleotide receptors triggers a strong activation of SAPK via a pathway independent of caspase-1- or caspase-3-like proteases.

* This work was supported in part by National Institutes of Health Grant GM36387.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Supported by Medical Scientist Training Program Grant GM07250-24 from the National Institutes of Health.

§ To whom correspondence should be addressed: Dept. Physiology and Biophysics, School of Medicine E565, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106-4970. Tel.: 216-368-5523; Fax: 216-368-3952; E-mail: gxd3@po.cwru.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
T. Noguchi, K. Ishii, H. Fukutomi, I. Naguro, A. Matsuzawa, K. Takeda, and H. Ichijo
Requirement of Reactive Oxygen Species-dependent Activation of ASK1-p38 MAPK Pathway for Extracellular ATP-induced Apoptosis in Macrophage
J. Biol. Chem., March 21, 2008; 283(12): 7657 - 7665.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
C. Guo, M. Masin, O. S. Qureshi, and R. D. Murrell-Lagnado
Evidence for Functional P2X4/P2X7 Heteromeric Receptors
Mol. Pharmacol., December 1, 2007; 72(6): 1447 - 1456.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. B. Choi, J. K. Ryu, S. U. Kim, and J. G. McLarnon
Modulation of the Purinergic P2X7 Receptor Attenuates Lipopolysaccharide-Mediated Microglial Activation and Neuronal Damage in Inflamed Brain
J. Neurosci., May 2, 2007; 27(18): 4957 - 4968.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. L. Fernando, B. M. Saunders, R. Sluyter, K. K. Skarratt, H. Goldberg, G. B. Marks, J. S. Wiley, and W. J. Britton
A Polymorphism in the P2X7 Gene Increases Susceptibility to Extrapulmonary Tuberculosis
Am. J. Respir. Crit. Care Med., February 15, 2007; 175(4): 360 - 366.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. D. Douillet, W. P. Robinson III, P. M. Milano, R. C. Boucher, and P. B. Rich
Nucleotides induce IL-6 release from human airway epithelia via P2Y2 and p38 MAPK-dependent pathways
Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L734 - L746.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Chen and C. F. Brosnan
Exacerbation of Experimental Autoimmune Encephalomyelitis in P2X7R-/- Mice: Evidence for Loss of Apoptotic Activity in Lymphocytes.
J. Immunol., March 1, 2006; 176(5): 3115 - 3126.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. N. Shemon, R. Sluyter, S. L. Fernando, A. L. Clarke, L.-P. Dao-Ung, K. K. Skarratt, B. M. Saunders, K. S. Tan, B. J. Gu, S. J. Fuller, et al.
A Thr357 to Ser Polymorphism in Homozygous and Compound Heterozygous Subjects Causes Absent or Reduced P2X7 Function and Impairs ATP-induced Mycobacterial Killing by Macrophages
J. Biol. Chem., January 27, 2006; 281(4): 2079 - 2086.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Kahlenberg, K. C. Lundberg, S. B. Kertesy, Y. Qu, and G. R. Dubyak
Potentiation of Caspase-1 Activation by the P2X7 Receptor Is Dependent on TLR Signals and Requires NF-{kappa}B-Driven Protein Synthesis
J. Immunol., December 1, 2005; 175(11): 7611 - 7622.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Tomioka, C. Sano, K. Sato, K. Ogasawara, T. Akaki, K. Sano, S. S. Cai, and T. Shimizu
Combined Effects of ATP on the Therapeutic Efficacy of Antimicrobial Drug Regimens against Mycobacterium avium Complex Infection in Mice and Roles of Cytosolic Phospholipase A2-Dependent Mechanisms in the ATP-Mediated Potentiation of Antimycobacterial Host Resistance
J. Immunol., November 15, 2005; 175(10): 6741 - 6749.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. B. Mackenzie, M. T. Young, E. Adinolfi, and A. Surprenant
Pseudoapoptosis Induced by Brief Activation of ATP-gated P2X7 Receptors
J. Biol. Chem., October 7, 2005; 280(40): 33968 - 33976.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Auger, I. Motta, K. Benihoud, D. M. Ojcius, and J. M. Kanellopoulos
A Role for Mitogen-activated Protein KinaseErk1/2 Activation and Non-selective Pore Formation in P2X7 Receptor-mediated Thymocyte Death
J. Biol. Chem., July 29, 2005; 280(30): 28142 - 28151.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. J. Sylte, C. J. Kuckleburg, T. J. Inzana, P. J. Bertics, and C. J. Czuprynski
Stimulation of P2X receptors enhances lipooligosaccharide-mediated apoptosis of endothelial cells
J. Leukoc. Biol., June 1, 2005; 77(6): 958 - 965.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y.-H. Feng, L. Wang, Q. Wang, X. Li, R. Zeng, and G. I. Gorodeski
ATP stimulates GRK-3 phosphorylation and {beta}-arrestin-2-dependent internalization of P2X7 receptor
Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1342 - C1356.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Bulanova, V. Budagian, Z. Orinska, M. Hein, F. Petersen, L. Thon, D. Adam, and S. Bulfone-Paus
Extracellular ATP Induces Cytokine Expression and Apoptosis through P2X7 Receptor in Murine Mast Cells
J. Immunol., April 1, 2005; 174(7): 3880 - 3890.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. C. Denlinger, G. Angelini, K. Schell, D. N. Green, A. G. Guadarrama, U. Prabhu, D. B. Coursin, P. J. Bertics, and K. Hogan
Detection of Human P2X7 Nucleotide Receptor Polymorphisms by a Novel Monocyte Pore Assay Predictive of Alterations in Lipopolysaccharide-Induced Cytokine Production
J. Immunol., April 1, 2005; 174(7): 4424 - 4431.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. Kuniyasu, S. Yano, T. Sasaki, T. Sasahira, S. Sone, and H. Ohmori
Colon Cancer Cell-Derived High Mobility Group 1/Amphoterin Induces Growth Inhibition and Apoptosis in Macrophages
Am. J. Pathol., March 1, 2005; 166(3): 751 - 760.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. X. Faria, F. P. DeFarias, and L. A. Alves
Are second messengers crucial for opening the pore associated with P2X7 receptor?
Am J Physiol Cell Physiol, February 1, 2005; 288(2): C260 - C271.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. A. Menze, M. J. Clavenna, and S. C. Hand
Depression of cell metabolism and proliferation by membrane-permeable and -impermeable modulators: role for AMP-to-ATP ratio
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2005; 288(2): R501 - R510.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Wang, Y.-H. Feng, and G. I. Gorodeski
Epidermal Growth Factor Facilitates Epinephrine Inhibition of P2X7-Receptor-Mediated Pore Formation and Apoptosis: A Novel Signaling Network
Endocrinology, January 1, 2005; 146(1): 164 - 174.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. D. Douillet, W. P. Robinson III, B. L. Zarzaur, E. R. Lazarowski, R. C. Boucher, and P. B. Rich
Mechanical Ventilation Alters Airway Nucleotides and Purinoceptors in Lung and Extrapulmonary Organs
Am. J. Respir. Cell Mol. Biol., January 1, 2005; 32(1): 52 - 58.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Q. Wang, L. Wang, Y.-H. Feng, X. Li, R. Zeng, and G. I. Gorodeski
P2X7 receptor-mediated apoptosis of human cervical epithelial cells
Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1349 - C1358.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Sluyter, A. N. Shemon, J. A. Barden, and J. S. Wiley
Extracellular ATP Increases Cation Fluxes in Human Erythrocytes by Activation of the P2X7 Receptor
J. Biol. Chem., October 22, 2004; 279(43): 44749 - 44755.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. M. Kahlenberg and G. R. Dubyak
Differing caspase-1 activation states in monocyte versus macrophage models of IL-1{beta} processing and release
J. Leukoc. Biol., September 1, 2004; 76(3): 676 - 684.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. J. Gu, R. Sluyter, K. K. Skarratt, A. N. Shemon, L.-P. Dao-Ung, S. J. Fuller, J. A. Barden, A. L. Clarke, S. Petrou, and J. S. Wiley
An Arg307 to Gln Polymorphism within the ATP-binding Site Causes Loss of Function of the Human P2X7 Receptor
J. Biol. Chem., July 23, 2004; 279(30): 31287 - 31295.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. M. Kahlenberg and G. R. Dubyak
Mechanisms of caspase-1 activation by P2X7 receptor-mediated K+ release
Am J Physiol Cell Physiol, May 1, 2004; 286(5): C1100 - C1108.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Le Stunff, R. Auger, J. Kanellopoulos, and M.-N. Raymond
The Pro-451 to Leu Polymorphism within the C-terminal Tail of P2X7 Receptor Impairs Cell Death but Not Phospholipase D Activation in Murine Thymocytes
J. Biol. Chem., April 23, 2004; 279(17): 16918 - 16926.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Aga, J. J. Watters, Z. A. Pfeiffer, G. J. Wiepz, J. A. Sommer, and P. J. Bertics
Evidence for nucleotide receptor modulation of cross talk between MAP kinase and NF-{kappa}B signaling pathways in murine RAW 264.7 macrophages
Am J Physiol Cell Physiol, April 1, 2004; 286(4): C923 - C930.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Sluyter, A. N. Shemon, and J. S. Wiley
Glu496 to Ala Polymorphism in the P2X7 Receptor Impairs ATP-Induced IL-1{beta} Release from Human Monocytes
J. Immunol., March 15, 2004; 172(6): 3399 - 3405.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
D. L. Donnelly-Roberts, M. T. Namovic, C. R. Faltynek, and M. F. Jarvis
Mitogen-Activated Protein Kinase and Caspase Signaling Pathways Are Required for P2X7 Receptor (P2X7R)-Induced Pore Formation in Human THP-1 Cells
J. Pharmacol. Exp. Ther., March 1, 2004; 308(3): 1053 - 1061.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. Suzuki, I. Hide, K. Ido, S. Kohsaka, K. Inoue, and Y. Nakata
Production and Release of Neuroprotective Tumor Necrosis Factor by P2X7 Receptor-Activated Microglia
J. Neurosci., January 7, 2004; 24(1): 1 - 7.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. M. Saunders, S. L. Fernando, R. Sluyter, W. J. Britton, and J. S. Wiley
A Loss-of-Function Polymorphism in the Human P2X7 Receptor Abolishes ATP-Mediated Killing of Mycobacteria
J. Immunol., November 15, 2003; 171(10): 5442 - 5446.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Adinolfi, M. Kim, M. T. Young, F. Di Virgilio, and A. Surprenant
Tyrosine Phosphorylation of HSP90 within the P2X7 Receptor Complex Negatively Regulates P2X7 Receptors
J. Biol. Chem., September 26, 2003; 278(39): 37344 - 37351.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. C. Denlinger, J. A. Sommer, K. Parker, L. Gudipaty, P. L. Fisette, J. W. Watters, R. A. Proctor, G. R. Dubyak, and P. J. Bertics
Mutation of a Dibasic Amino Acid Motif Within the C Terminus of the P2X7 Nucleotide Receptor Results in Trafficking Defects and Impaired Function
J. Immunol., August 1, 2003; 171(3): 1304 - 1311.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Gudipaty, J. Munetz, P. A. Verhoef, and G. R. Dubyak
Essential role for Ca2+ in regulation of IL-1{beta} secretion by P2X7 nucleotide receptor in monocytes, macrophages, and HEK-293 cells
Am J Physiol Cell Physiol, August 1, 2003; 285(2): C286 - C299.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
H. Z. Ke, H. Qi, A. F. Weidema, Q. Zhang, N. Panupinthu, D. T. Crawford, W. A. Grasser, V. M. Paralkar, M. Li, L. P. Audoly, et al.
Deletion of the P2X7 Nucleotide Receptor Reveals Its Regulatory Roles in Bone Formation and Resorption
Mol. Endocrinol., July 1, 2003; 17(7): 1356 - 1367.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. A. Verhoef, M. Estacion, W. Schilling, and G. R. Dubyak
P2X7 Receptor-Dependent Blebbing and the Activation of Rho-Effector Kinases, Caspases, and IL-1{beta} Release
J. Immunol., June 1, 2003; 170(11): 5728 - 5738.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. E. Laliberte, D. G. Perregaux, L. R. Hoth, P. J. Rosner, C. K. Jordan, K. M. Peese, James. F. Eggler, M. A. Dombroski, K. F. Geoghegan, and C. A. Gabel
Glutathione S-Transferase Omega 1-1 Is a Target of Cytokine Release Inhibitory Drugs and May Be Responsible for Their Effect on Interleukin-1beta Posttranslational Processing
J. Biol. Chem., May 2, 2003; 278(19): 16567 - 16578.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. S. Wiley, L.-P. Dao-Ung, C. Li, A. N. Shemon, B. J. Gu, M. L. Smart, S. J. Fuller, J. A. Barden, S. Petrou, and R. Sluyter
An Ile-568 to Asn Polymorphism Prevents Normal Trafficking and Function of the Human P2X7 Receptor
J. Biol. Chem., May 2, 2003; 278(19): 17108 - 17113.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
L. T. Wen, C. C. Caldwell, and A. F. Knowles
Poly(ADP-ribose) Polymerase Activation and Changes in Bax Protein Expression Associated with Extracellular ATP-Mediated Apoptosis in Human Embryonic Kidney 293-P2X7 Cells
Mol. Pharmacol., March 1, 2003; 63(3): 706 - 713.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
F.-P. Gendron, J. T. Neary, P. M. Theiss, G. Y. Sun, F. A. Gonzalez, and G. A. Weisman
Mechanisms of P2X7 receptor-mediated ERK1/2 phosphorylation in human astrocytoma cells
Am J Physiol Cell Physiol, February 1, 2003; 284(2): C571 - C581.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Budagian, E. Bulanova, L. Brovko, Z. Orinska, R. Fayad, R. Paus, and S. Bulfone-Paus
Signaling through P2X7 Receptor in Human T Cells Involves p56lck, MAP Kinases, and Transcription Factors AP-1 and NF-kappa B
J. Biol. Chem., January 10, 2003; 278(3): 1549 - 1560.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. L. Wilson, S. A. Wilson, A. Surprenant, and R. A. North
Epithelial Membrane Proteins Induce Membrane Blebbing and Interact with the P2X7 Receptor C Terminus
J. Biol. Chem., September 6, 2002; 277(37): 34017 - 34023.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
M. Ryten, P. M. Dunn, J. T. Neary, and G. Burnstock
ATP regulates the differentiation of mammalian skeletal muscle by activation of a P2X5 receptor on satellite cells
J. Cell Biol., July 22, 2002; 158(2): 345 - 355.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. Aga, C. J. Johnson, A. P. Hart, A. G. Guadarrama, M. Suresh, J. Svaren, P. J. Bertics, and B. J. Darien
Modulation of monocyte signaling and pore formation in response to agonists of the nucleotide receptor P2X7
J. Leukoc. Biol., July 1, 2002; 72(1): 222 - 232.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Labasi, N. Petrushova, C. Donovan, S. McCurdy, P. Lira, M. M. Payette, W. Brissette, J. R. Wicks, L. Audoly, and C. A. Gabel
Absence of the P2X7 Receptor Alters Leukocyte Function and Attenuates an Inflammatory Response
J. Immunol., June 15, 2002; 168(12): 6436 - 6445.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. Chakfe, R. Seguin, J. P. Antel, C. Morissette, D. Malo, D. Henderson, and P. Seguela
ADP and AMP Induce Interleukin-1beta Release from Microglial Cells through Activation of ATP-Primed P2X7 Receptor Channels
J. Neurosci., April 15, 2002; 22(8): 3061 - 3069.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. G. Perregaux, K. Bhavsar, L. Contillo, J. Shi, and C. A. Gabel
Antimicrobial Peptides Initiate IL-1{beta} Posttranslational Processing: A Novel Role Beyond Innate Immunity
J. Immunol., March 15, 2002; 168(6): 3024 - 3032.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. A. Le Feuvre, D. Brough, Y. Iwakura, K. Takeda, and N. J. Rothwell
Priming of Macrophages with Lipopolysaccharide Potentiates P2X7-mediated Cell Death via a Caspase-1-dependent Mechanism, Independently of Cytokine Production
J. Biol. Chem., January 25, 2002; 277(5): 3210 - 3218.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. I. Gorodeski
Expression, regulation, and function of P2X4 purinergic receptor in human cervical epithelial cells
Am J Physiol Cell Physiol, January 1, 2002; 282(1): C84 - C93.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. C. Denlinger, P. L. Fisette, J. A. Sommer, J. J. Watters, U. Prabhu, G. R. Dubyak, R. A. Proctor, and P. J. Bertics
Cutting Edge: The Nucleotide Receptor P2X7 Contains Multiple Protein- and Lipid-Interaction Motifs Including a Potential Binding Site for Bacterial Lipopolysaccharide
J. Immunol., August 15, 2001; 167(4): 1871 - 1876.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. R. John, J. E. Simpson, M. N. Woodroofe, S. C. Lee, and C. F. Brosnan
Extracellular Nucleotides Differentially Regulate Interleukin-1{beta} Signaling in Primary Human Astrocytes: Implications for Inflammatory Gene Expression
J. Neurosci., June 15, 2001; 21(12): 4134 - 4142.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B.-C. Suh, J.-S. Kim, U. Namgung, H. Ha, and K.-T. Kim
P2X7 Nucleotide Receptor Mediation of Membrane Pore Formation and Superoxide Generation in Human Promyelocytes and Neutrophils
J. Immunol., June 1, 2001; 166(11): 6754 - 6763.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. D. Beigi and G. R. Dubyak
Endotoxin Activation of Macrophages Does Not Induce ATP Release and Autocrine Stimulation of P2 Nucleotide Receptors
J. Immunol., December 15, 2000; 165(12): 7189 - 7198.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. A. Sellers, J. Simon, T. S. Lundahl, D. J. Cousens, P. P. A. Humphrey, and E. A. Barnard
Adenosine Nucleotides Acting at the Human P2Y1 Receptor Stimulate Mitogen-activated Protein Kinases and Induce Apoptosis
J. Biol. Chem., May 4, 2001; 276(19): 16379 - 16390.
[Abstract] [Full Text] [PDF]