JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M002957200 on June 23, 2000

J. Biol. Chem., Vol. 275, Issue 35, 27316-27323, September 1, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/35/27316    most recent
M002957200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kirschnek, S.
Right arrow Articles by Gulbins, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kirschnek, S.
Right arrow Articles by Gulbins, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

CD95-mediated Apoptosis in Vivo Involves Acid Sphingomyelinase*

Susanne Kirschnek, Francois ParisDagger , Michael Weller§, Heike Grassmé, Klaus Ferlinz, Andrea Riehle, Zvi FuksDagger , Richard Kolesnick, and Erich Gulbins||

From the Department of Physiology, University of Tuebingen, Gmelinstrasse 5 and § Department of Neurology, University of Tuebingen, Hoppe-Seyler-Strasse 3, 72076 Tuebingen, Germany and the  Laboratory of Signal Transduction and Dagger  Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021

Acid sphingomyelinase (ASM) is reported to have an essential function in stress-induced apoptosis although the physiological function of ASM in receptor-triggered apoptosis is unknown. Here, we delineate a pivotal role for ASM in CD95-triggered apoptosis of peripheral lymphocytes or hepatocytes in vivo. We employed intravenous injection of anti-CD4 antibodies or phytohemagglutinin that was previously shown to result in apoptosis of peripheral blood lymphocytes or hepatocytes via the endogenous CD95/CD95 ligand system. Our results demonstrate a high susceptibility in normal mice whereas ASM knock-out mice fail to immunodeplete T cells or develop autoimmune-like hepatitis. Likewise, ASM-deficient mice or hepatocytes and splenocytes ex vivo manifest resistance to anti-CD95 treatment. These results provide in vivo evidence for an important physiological function of ASM in CD95-induced apoptosis.

* The study was supported in part by Deutsche Forschungsgemeinschaft Grant GU 335/2-3, grants from the Association International Cancer Research, the Interdisciplinary Center for Clinical Research (to E. G.) and the Mildred Scheel Stiftung Grant 1502/5-2 (to M. W. and E. G.), and by National Institutes of Health Grants CA52462 (to Z. F.) and CA42385 (to R. K.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed. Tel.: 49-7071-2972196; Fax: 49-7071-293073; E-mail: erich.gulbins@uni- tuebingen.de.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
R. Reinehr, A. Sommerfeld, V. Keitel, S. Grether-Beck, and D. Haussinger
Amplification of CD95 Activation by Caspase 8-induced Endosomal Acidification in Rat Hepatocytes
J. Biol. Chem., January 25, 2008; 283(4): 2211 - 2222.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Chentouf, S. Ghannam, C. Bes, S. Troadec, M. Cerutti, and T. Chardes
Recombinant Anti-CD4 Antibody 13B8.2 Blocks Membrane-Proximal Events by Excluding the Zap70 Molecule and Downstream Targets SLP-76, PLC{gamma}1, and Vav-1 from the CD4-Segregated Brij 98 Detergent-Resistant Raft Domains
J. Immunol., July 1, 2007; 179(1): 409 - 420.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Reinehr, S. Becker, J. Braun, A. Eberle, S. Grether-Beck, and D. Haussinger
Endosomal Acidification and Activation of NADPH Oxidase Isoforms Are Upstream Events in Hyperosmolarity-induced Hepatocyte Apoptosis
J. Biol. Chem., August 11, 2006; 281(32): 23150 - 23166.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
E. Gulbins and P. L. Li
Physiological and pathophysiological aspects of ceramide
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R11 - R26.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
J. Wu, Y. Cheng, B. A. G. Jonsson, A. Nilsson, and R.-D. Duan
Acid sphingomyelinase is induced by butyrate but does not initiate the anticancer effect of butyrate in HT29 and HepG2 cells
J. Lipid Res., September 1, 2005; 46(9): 1944 - 1952.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Reinehr, S. Becker, A. Eberle, S. Grether-Beck, and D. Haussinger
Involvement of NADPH Oxidase Isoforms and Src Family Kinases in CD95-dependent Hepatocyte Apoptosis
J. Biol. Chem., July 22, 2005; 280(29): 27179 - 27194.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Grassme, A. Riehle, B. Wilker, and E. Gulbins
Rhinoviruses Infect Human Epithelial Cells via Ceramide-enriched Membrane Platforms
J. Biol. Chem., July 15, 2005; 280(28): 26256 - 26262.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. A. Rotolo, J. Zhang, M. Donepudi, H. Lee, Z. Fuks, and R. Kolesnick
Caspase-dependent and -independent Activation of Acid Sphingomyelinase Signaling
J. Biol. Chem., July 15, 2005; 280(28): 26425 - 26434.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Falcone, C. Perrotta, C. De Palma, A. Pisconti, C. Sciorati, A. Capobianco, P. Rovere-Querini, A. A. Manfredi, and E. Clementi
Activation of Acid Sphingomyelinase and Its Inhibition by the Nitric Oxide/Cyclic Guanosine 3',5'-Monophosphate Pathway: Key Events in Escherichia coli-Elicited Apoptosis of Dendritic Cells
J. Immunol., October 1, 2004; 173(7): 4452 - 4463.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Gupta, R. Natarajan, S. G. Payne, E. J. Studer, S. Spiegel, P. Dent, and P. B. Hylemon
Deoxycholic Acid Activates the c-Jun N-terminal Kinase Pathway via FAS Receptor Activation in Primary Hepatocytes: ROLE OF ACIDIC SPHINGOMYELINASE-MEDIATED CERAMIDE GENERATION IN FAS RECEPTOR ACTIVATION
J. Biol. Chem., February 13, 2004; 279(7): 5821 - 5828.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
I. Silins, M. Nordstrand, J. Hogberg, and U. Stenius
Sphingolipids suppress preneoplastic rat hepatocytes in vitro and in vivo
Carcinogenesis, June 1, 2003; 24(6): 1077 - 1083.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Marchesini, C. Luberto, and Y. A. Hannun
Biochemical Properties of Mammalian Neutral Sphingomyelinase2 and Its Role in Sphingolipid Metabolism
J. Biol. Chem., April 11, 2003; 278(16): 13775 - 13783.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. K. Pru, I. R. Hendry, J. S. Davis, and B. R. Rueda
Soluble Fas Ligand Activates the Sphingomyelin Pathway and Induces Apoptosis in Luteal Steroidogenic Cells Independently of Stress-Activated p38MAPK
Endocrinology, November 1, 2002; 143(11): 4350 - 4357.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Luberto, D. F. Hassler, P. Signorelli, Y. Okamoto, H. Sawai, E. Boros, D. J. Hazen-Martin, L. M. Obeid, Y. A. Hannun, and G. K. Smith
Inhibition of Tumor Necrosis Factor-induced Cell Death in MCF7 by a Novel Inhibitor of Neutral Sphingomyelinase
J. Biol. Chem., October 18, 2002; 277(43): 41128 - 41139.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
Y.-W. Hsueh, R. Giles, N. Kitson, and J. Thewalt
The Effect of Ceramide on Phosphatidylcholine Membranes: A Deuterium NMR Study
Biophys. J., June 1, 2002; 82(6): 3089 - 3095.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. Loidl, R. Claus, H. P. Deigner, and A. Hermetter
High-precision fluorescence assay for sphingomyelinase activity of isolated enzymes and cell lysates
J. Lipid Res., May 1, 2002; 43(5): 815 - 823.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Paris, H. Grassme, A. Cremesti, J. Zager, Y. Fong, A. Haimovitz-Friedman, Z. Fuks, E. Gulbins, and R. Kolesnick
Natural Ceramide Reverses Fas Resistance of Acid Sphingomyelinase-/- Hepatocytes
J. Biol. Chem., March 9, 2001; 276(11): 8297 - 8305.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Cremesti, F. Paris, H. Grassme, N. Holler, J. Tschopp, Z. Fuks, E. Gulbins, and R. Kolesnick
Ceramide Enables Fas to Cap and Kill
J. Biol. Chem., June 22, 2001; 276(26): 23954 - 23961.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. P. de Chaves, M. Bussiere, B. MacInnis, D. E. Vance, R. B. Campenot, and J. E. Vance
Ceramide Inhibits Axonal Growth and Nerve Growth Factor Uptake without Compromising the Viability of Sympathetic Neurons
J. Biol. Chem., September 21, 2001; 276(39): 36207 - 36214.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.