HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 275, Issue 35, 27366-27376, September 1, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/35/27366    most recent M000405200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, Y.-H. Articles by Ramos, K. S. Search for Related Content PubMed PubMed Citation Articles by Chen, Y.-H. Articles by Ramos, K. S. Social Bookmarking                      What's this?

A CCAAT/Enhancer-binding Protein Site within Antioxidant/Electrophile Response Element Along with CREB-binding Protein Participate in the Negative Regulation of Rat GST-Ya Gene in Vascular Smooth Muscle Cells^*

Yun-Houng Chen and Kenneth S. Ramos

From the Department of Physiology and Pharmacology, College of Veterinary Medicine, and Center for Environmental and Rural Health, Texas A & M University, College Station, Texas 77843 4466

Studies were conducted to evaluate the negative regulatory function of rat (r)GST-Ya antioxidant/electrophile response element (ARE/EpRE) in vascular smooth muscle cells (vSMCs). We report that CCAAT/enhancer-binding protein (C/EBP)- interacts with ARE/EpRE in the rGST-Ya promoter and that aryl hydrocarbon receptor (AhR) is present within the protein complex binding to the C/EBP site. Overexpression of C/EBP- or C/EBP- repressed, whereas AhR enhanced, 1.6CAT reporter activity in cells treated with benzo(a)pyrene (BaP). Overexpression of CREB-binding protein (CBP) nullified repression of rGST-Ya transcription. Human adenovirus E1A protein abrogated cotransactivation by CBP but an E1A mutant did not. Overexpression of C/EBPs abrogated stimulation of 1.6CAT by CBP or AhR alone, or in combination, regardless of BaP treatment. Similar profiles were observed using an AhRECAT construct. The C/EBP site within the ARE/EpRE inhibited chemical inducibility of the AhRE. The pattern of mouse GST-Ya regulation by BaP was similar to that of rGST-Ya. We conclude that multiple mechanisms mediate negative regulation of GST-Ya gene in vSMCs, most significant of which are that C/EBP- inhibits AhRE or ARE/EpRE inducibility of GST-Ya, limiting CBP levels compromise gene induction, functional interference exists between AhRE and ARE/EpRE, and AhR alone, or in combination with C/EBP-, functions as a repressor of the ARE/EpRE.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				K. P. Lu and K. S. Ramos Redox Regulation of a Novel L1Md-A2 Retrotransposon in Vascular Smooth Muscle Cells J. Biol. Chem., July 18, 2003; 278(30): 28201 - 28209. [Abstract] [Full Text] [PDF]

				C. D. Johnson, Y. Balagurunathan, K. P. Lu, M. Tadesse, M. H. Falahatpisheh, R. J. Carroll, E. R. Dougherty, C. A. Afshari, and K. S. Ramos Genomic profiles and predictive biological networks in oxidant-induced atherogenesis Physiol Genomics, May 13, 2003; 13(3): 263 - 275. [Abstract] [Full Text] [PDF]

				J.-M. Lee, M. J. Calkins, K. Chan, Y. W. Kan, and J. A. Johnson Identification of the NF-E2-related Factor-2-dependent Genes Conferring Protection against Oxidative Stress in Primary Cortical Astrocytes Using Oligonucleotide Microarray Analysis J. Biol. Chem., March 28, 2003; 278(14): 12029 - 12038. [Abstract] [Full Text] [PDF]

				S. Yang, Z. Fang, T. Suzuki, H. Sasano, J. Zhou, B. Gurates, M. Tamura, K. Ferrer, and S. Bulun Regulation of Aromatase P450 Expression in Endometriotic and Endometrial Stromal Cells by CCAAT/Enhancer Binding Proteins (C/EBPs): Decreased C/EBP{beta} in Endometriosis Is Associated with Overexpression of Aromatase J. Clin. Endocrinol. Metab., May 1, 2002; 87(5): 2336 - 2345. [Abstract] [Full Text] [PDF]

				R. N. Hines, Z. Luo, T. Cresteil, X. Ding, R. A. Prough, J. L. Fitzpatrick, S. L. Ripp, K. C. Falkner, N.-L. Ge, A. Levine, et al. Molecular Regulation of Genes Encoding Xenobiotic-Metabolizing Enzymes: Mechanisms Involving Endogenous Factors Drug Metab. Dispos., April 13, 2001; 29(5): 623 - 633. [Abstract] [Full Text]

				J. K. Kerzee and K. S. Ramos Constitutive and Inducible Expression of Cyp1a1 and Cyp1b1 in Vascular Smooth Muscle Cells: Role of the Ahr bHLH/PAS Transcription Factor Circ. Res., September 28, 2001; 89(7): 573 - 582. [Abstract] [Full Text] [PDF]