JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M004810200 on June 12, 2000

J. Biol. Chem., Vol. 275, Issue 35, 27414-27420, September 1, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/35/27414    most recent
M004810200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adams, A.
Right arrow Articles by O'Bryan, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adams, A.
Right arrow Articles by O'Bryan, J. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Intersectin, an Adaptor Protein Involved in Clathrin-mediated Endocytosis, Activates Mitogenic Signaling Pathways*

Anthony AdamsDagger , Judith M. ThornDagger , Montarop Yamabhai§, Brian K. Kay§, and John P. O'BryanDagger ||

From the Dagger  Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709 and the § Department of Pharmacology, University of Wisconsin, Madison, Wisconsin 53706

Intersectin is a member of a growing family of adaptor proteins that possess conserved Eps15 homology (EH) domains as well as additional protein recognition motifs. In general, EH domain-containing proteins play an integral role in clathrin-mediated endocytosis. Indeed, intersectin functions in the intermediate stages of clathrin-coated vesicle assembly. However, recent evidence suggests that components of the endocytic machinery also regulate mitogenic signaling pathways. In this report, we provide several lines of evidence that intersectin has the capacity to activate mitogenic signaling pathways. First, intersectin overexpression activated the Elk-1 transcription factor in an MAPK-independent manner. This ability resides within the EH domains, as expression of the tandem EH domains was sufficient to activate Elk-1. Second, intersectin cooperated with epidermal growth factor to potentiate Elk-1 activation; however, a similar level of Elk-1 activation was obtained by expression of the tandem EH domains suggesting that the coiled-coil region and SH3 domains act to regulate the EH domains. Third, intersectin expression was sufficient to induce oncogenic transformation of rodent fibroblasts. And finally, intersectin cooperated with progesterone to accelerate maturation of Xenopus laevis oocytes. Together, these data suggest that intersectin links endocytosis with regulation of pathways important for cell growth and differentiation.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Dept. of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235.

|| To whom correspondence should be addressed: National Institute of Environmental Health Sciences, National Institutes of Health, Bldg. 101, Rm. F336, MD F3-06, P.O. Box 12233, Research Triangle Park, NC 27709. Tel.: 919-541-3619; Fax: 919-541-1898; E-mail: obryan@niehs.nih.gov.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
Y. Yu, P.-Y. Chu, D. N. Bowser, D. J. Keating, D. Dubach, I. Harper, J. Tkalcevic, D. I. Finkelstein, and M. A. Pritchard
Mice deficient for the chromosome 21 ortholog Itsn1 exhibit vesicle-trafficking abnormalities
Hum. Mol. Genet., November 1, 2008; 17(21): 3281 - 3290.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Chabu and C. Q. Doe
Dap160/intersectin binds and activates aPKC to regulate cell polarity and cell cycle progression
Development, August 15, 2008; 135(16): 2739 - 2746.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Das, E. Scappini, N. P. Martin, K. A. Wong, S. Dunn, Y.-J. Chen, S. L. H. Miller, J. Domin, and J. P. O'Bryan
Regulation of Neuron Survival through an Intersectin-Phosphoinositide 3'-Kinase C2{beta}-AKT Pathway
Mol. Cell. Biol., November 15, 2007; 27(22): 7906 - 7917.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
E. Scappini, T.-W. Koh, N. P. Martin, and J. P. O'Bryan
Intersectin enhances huntingtin aggregation and neurodegeneration through activation of c-Jun-NH2-terminal kinase
Hum. Mol. Genet., August 1, 2007; 16(15): 1862 - 1871.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. A. Predescu, D. N. Predescu, I. Knezevic, I. K. Klein, and A. B. Malik
Intersectin-1s Regulates the Mitochondrial Apoptotic Pathway in Endothelial Cells
J. Biol. Chem., June 8, 2007; 282(23): 17166 - 17178.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
N. P. Martin, R. P. Mohney, S. Dunn, M. Das, E. Scappini, and J. P. O'Bryan
Intersectin Regulates Epidermal Growth Factor Receptor Endocytosis, Ubiquitylation, and Signaling
Mol. Pharmacol., November 1, 2006; 70(5): 1643 - 1653.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Naslavsky and S. Caplan
C-terminal EH-domain-containing proteins: consensus for a role in endocytic trafficking, EH?
J. Cell Sci., September 15, 2005; 118(18): 4093 - 4101.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-B. Wang, W. J. Wu, and R. A. Cerione
Cdc42 and Ras Cooperate to Mediate Cellular Transformation by Intersectin-L
J. Biol. Chem., June 17, 2005; 280(24): 22883 - 22891.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Kolli, C. I. Zito, M. H. Mossink, E. A. C. Wiemer, and A. M. Bennett
The Major Vault Protein Is a Novel Substrate for the Tyrosine Phosphatase SHP-2 and Scaffold Protein in Epidermal Growth Factor Signaling
J. Biol. Chem., July 9, 2004; 279(28): 29374 - 29385.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. W. P. Yam, D.-Y. Jin, C. W. So, and L. C. Chan
Identification and characterization of EBP, a novel EEN binding protein that inhibits Ras signaling and is recruited into the nucleus by the MLL-EEN fusion protein
Blood, February 15, 2004; 103(4): 1445 - 1453.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
S. Polo, S. Confalonieri, A. E. Salcini, and P. P. Di Fiore
EH and UIM: Endocytosis and More
Sci. Signal., December 16, 2003; 2003(213): re17 - re17.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. A. Predescu, D. N. Predescu, B. K. Timblin, R. V. Stan, and A. B. Malik
Intersectin Regulates Fission and Internalization of Caveolae in Endothelial Cells
Mol. Biol. Cell, December 1, 2003; 14(12): 4997 - 5010.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. P. Mohney, M. Das, T. G. Bivona, R. Hanes, A. G. Adams, M. R. Philips, and J. P. O'Bryan
Intersectin Activates Ras but Stimulates Transcription through an Independent Pathway Involving JNK
J. Biol. Chem., November 21, 2003; 278(47): 47038 - 47045.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Jenna, N. K. Hussain, E. I. Danek, I. Triki, S. Wasiak, P. S. McPherson, and N. Lamarche-Vane
The Activity of the GTPase-activating Protein CdGAP Is Regulated by the Endocytic Protein Intersectin
J. Biol. Chem., February 15, 2002; 277(8): 6366 - 6373.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
M. K.H. McGavin, K. Badour, L. A. Hardy, T. J. Kubiseski, J. Zhang, and K. A. Siminovitch
The Intersectin 2 Adaptor Links Wiskott Aldrich Syndrome Protein (WASp)-mediated Actin Polymerization to T Cell Antigen Receptor Endocytosis
J. Exp. Med., December 17, 2001; 194(12): 1777 - 1787.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-K. Tong, N. K. Hussain, A. G. Adams, J. P. O'Bryan, and P. S. McPherson
Intersectin Can Regulate the Ras/MAP Kinase Pathway Independent of Its Role in Endocytosis
J. Biol. Chem., September 15, 2000; 275(38): 29894 - 29899.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Rotem-Yehudar, E. Galperin, and M. Horowitz
Association of Insulin-like Growth Factor 1 Receptor with EHD1 and SNAP29
J. Biol. Chem., August 24, 2001; 276(35): 33054 - 33060.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. R. Ramjaun, A. Angers, V. Legendre-Guillemin, X.-K. Tong, and P. S. McPherson
Endophilin Regulates JNK Activation through Its Interaction with the Germinal Center Kinase-like Kinase
J. Biol. Chem., July 27, 2001; 276(31): 28913 - 28919.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Ringstad, Y. Nemoto, and P. De Camilli
Differential Expression of Endophilin 1 and 2 Dimers at Central Nervous System Synapses
J. Biol. Chem., October 26, 2001; 276(44): 40424 - 40430.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.