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J. Biol. Chem., Vol. 275, Issue 36, 27689-27693, September 8, 2000
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From the Aggregation of proteins is a problem with serious
medical implications and economic importance. To develop strategies for preventing aggregation, the mechanism(s) and pathways by which proteins
aggregate must be characterized. In this study, the thermally induced
aggregation processes of three
Entrapping Intermediates of Thermal Aggregation in
-Helical
Proteins with Low Concentration of Guanidine Hydrochloride*
§,
Department of Chemistry and Biochemistry,
University of Northern Colorado, Greeley, Colorado 80639, the
¶ Department of Chemical Engineering, University of Colorado,
Boulder, Colorado 80309, and the Department of Pharmaceutical
Sciences, School of Pharmacy, University of Colorado Health Sciences
Center, Denver, Colorado 80262
-helix proteins (myoglobin, cytochrome c, and lysozyme) in the presence and absence of
1.0 M guanidine hydrochloride (GdnHCl) were investigated by
means of infrared spectroscopy. In the absence of GdnHCl, intensities of the -helix bands (~1656 cm
1) decrease as a
function of temperature at above 50 °C. With myoglobin and
cytochrome c, the loss of helix bands was accompanied by
the appearance of two new bands at 1694 and 1623 cm1,
indicative of the formation of intermolecular 
-sheet aggregates. For
lysozyme, bands indicative of intermolecular -sheet aggregates did
not appear in any significant intensity. In the presence of 1.0 M GdnHCl, two major intermediate states rich in
310-helix (represented by the band at 1663 cm1) and -turn structure (represented by the band at
1667 cm1), respectively, were observed. These findings
demonstrated that IR spectroscopic studies of protein aggregation using
a combination of thermal and chemical denaturing factors could provide
a means to populate and characterize aggregation intermediates.
*
This work was supported in part by National Institutes of
Health Grant 1R15GM5588901 (to A. D.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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