|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 36, 27838-27844, September 8, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Departments of We have shown previously that partial inhibition
of the cardiac myocyte Na+/K+-ATPase
activates signal pathways that regulate myocyte growth and
growth-related genes and that increases in intracellular
Ca2+ concentration ([Ca2+]i) and
reactive oxygen species (ROS) are two essential second messengers
within these pathways. The aim of this work was to explore the relation
between [Ca2+]i and ROS. When myocytes were in a
Ca2+-free medium, ouabain caused no change in
[Ca2+]i, but it increased ROS as it did when the
cells were in a Ca2+-containing medium. Ouabain-induced
increase in ROS also occurred under conditions where there was little
or no change in [Na+]i. Exposure of myocytes in
Ca2+-free medium to monensin did not increase ROS. Increase
in protein tyrosine phosphorylation, an early event induced by ouabain,
was also independent of changes in [Ca2+]i and
[Na+]i. Ouabain-induced generation of ROS in
myocytes was antagonized by genistein, a dominant negative Ras, and
myxothiazol/diphenyleneiodonium, indicating a mitochondrial origin for
the Ras-dependent ROS generation. These findings, along
with our previous data, indicate that increases in
[Ca2+]i and ROS in cardiac myocytes are induced
by two parallel pathways initiated at the plasma membrane: One being
the ouabain-altered transient interactions of a fraction of the
Na+/K+-ATPase with neighboring proteins (Src,
growth factor receptors, adaptor proteins, and Ras) leading to ROS
generation, and the other, inhibition of the transport function of
another fraction of the Na+/K+-ATPase leading
to rise in [Ca2+]i. Evidently, the gene
regulatory effects of ouabain in cardiac myocytes require the
downstream collaborations of ROS and
[Ca2+]i.
Ouabain Interaction with Cardiac
Na+/K+-ATPase Initiates Signal Cascades
Independent of Changes in Intracellular Na+ and
Ca2+ Concentrations*
,,,, and¶
Pharmacology and
§ Medicine, Medical College of Ohio, Toledo, Ohio
43614
*
This work was supported by Grants HL-36573 and HL-63238
awarded by the NHLBI, National Institutes of Health, United
States Public Health Service, Department of Health and Human Services.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Wang, A. T. Dennis, P. Trieu, F. Charron, N. Ethier, T. E. Hebert, X. Wan, and E. Ficker Intracellular Potassium Stabilizes Human Ether-a-go-go-Related Gene Channels for Export from Endoplasmic Reticulum Mol. Pharmacol., April 1, 2009; 75(4): 927 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y. Bagrov, J. I. Shapiro, and O. V. Fedorova Endogenous Cardiotonic Steroids: Physiology, Pharmacology, and Novel Therapeutic Targets Pharmacol. Rev., March 1, 2009; 61(1): 9 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Stoilov, C.-H. Lin, R. Damoiseaux, J. Nikolic, and D. L. Black A high-throughput screening strategy identifies cardiotonic steroids as alternative splicing modulators PNAS, August 12, 2008; 105(32): 11218 - 11223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. V. Pierre, Y. Sottejeau, J.-M. Gourbeau, G. Sanchez, A. Shidyak, and G. Blanco Isoform specificity of Na-K-ATPase-mediated ouabain signaling Am J Physiol Renal Physiol, April 1, 2008; 294(4): F859 - F866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Newman, P. Yang, A. D. Pawlus, and K. I. Block Cardiac Glycosides as Novel Cancer Therapeutic Agents Mol. Interv., February 1, 2008; 8(1): 36 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Cai, L. Wu, W. Qu, D. Malhotra, Z. Xie, J. I. Shapiro, and J. Liu Regulation of apical NHE3 trafficking by ouabain-induced activation of the basolateral Na+-K+-ATPase receptor complex Am J Physiol Cell Physiol, February 1, 2008; 294(2): C555 - C563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu, X. Zhao, S. V. Pierre, and A. Askari Association of PI3K-Akt signaling pathway with digitalis-induced hypertrophy of cardiac myocytes Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1489 - C1497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Khundmiri, V. Amin, J. Henson, J. Lewis, M. Ameen, M. J. Rane, and N. A. Delamere Ouabain stimulates protein kinase B (Akt) phosphorylation in opossum kidney proximal tubule cells through an ERK-dependent pathway Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1171 - C1180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Schoner and G. Scheiner-Bobis Endogenous and exogenous cardiac glycosides: their roles in hypertension, salt metabolism, and cell growth Am J Physiol Cell Physiol, August 1, 2007; 293(2): C509 - C536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Kaunitz Reply to Blaustein et al. Am J Physiol Renal Physiol, July 1, 2007; 293(1): F439 - F439. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Liang, J. Tian, L. Liu, S. Pierre, J. Liu, J. Shapiro, and Z.-J. Xie Identification of a Pool of Non-pumping Na/K-ATPase J. Biol. Chem., April 6, 2007; 282(14): 10585 - 10593. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Pasdois, C. L. Quinlan, A. Rissa, L. Tariosse, B. Vinassa, A. D. T. Costa, S. V. Pierre, P. Dos Santos, and K. D. Garlid Ouabain protects rat hearts against ischemia-reperfusion injury via pathway involving src kinase, mitoKATP, and ROS Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1470 - H1478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. V. Pierre, C. Yang, Z. Yuan, J. Seminerio, C. Mouas, K. D. Garlid, P. Dos-Santos, and Z. Xie Ouabain triggers preconditioning through activation of the Na+,K+-ATPase signaling cascade in rat hearts Cardiovasc Res, February 1, 2007; 73(3): 488 - 496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Elkareh, D. J. Kennedy, B. Yashaswi, S. Vetteth, A. Shidyak, E. G. R. Kim, S. Smaili, S. M. Periyasamy, I. M. Hariri, L. Fedorova, et al. Marinobufagenin Stimulates Fibroblast Collagen Production and Causes Fibrosis in Experimental Uremic Cardiomyopathy Hypertension, January 1, 2007; 49(1): 215 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Khundmiri, M. A. Metzler, M. Ameen, V. Amin, M. J. Rane, and N. A. Delamere Ouabain induces cell proliferation through calcium-dependent phosphorylation of Akt (protein kinase B) in opossum kidney proximal tubule cells Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1247 - C1257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Kennedy, S. Vetteth, M. Xie, S. M. Periyasamy, Z. Xie, C. Han, V. Basrur, K. Mutgi, V. Fedorov, D. Malhotra, et al. Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H3003 - H3011. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Thundathil, M. Anzar, and M. M. Buhr Na+/K+ATPase as a Signaling Molecule During Bovine Sperm Capacitation Biol Reprod, September 1, 2006; 75(3): 308 - 317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liang, T. Cai, J. Tian, W. Qu, and Z.-J. Xie Functional Characterization of Src-interacting Na/K-ATPase Using RNA Interference Assay J. Biol. Chem., July 14, 2006; 281(28): 19709 - 19719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Frese, M. Frese-Schaper, A.-C. Andres, D. Miescher, B. Zumkehr, and R. A. Schmid Cardiac Glycosides Initiate Apo2L/TRAIL-Induced Apoptosis in Non-Small Cell Lung Cancer Cells by Up-regulation of Death Receptors 4 and 5 Cancer Res., June 1, 2006; 66(11): 5867 - 5874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Kaunitz Membrane transport proteins: not just for transport anymore Am J Physiol Renal Physiol, May 1, 2006; 290(5): F995 - F996. [Full Text] [PDF]
|
|
|
|
|
|
S. Oweis, L. Wu, P. R. Kiela, H. Zhao, D. Malhotra, F. K. Ghishan, Z. Xie, J. I. Shapiro, and J. Liu Cardiac glycoside downregulates NHE3 activity and expression in LLC-PK1 cells Am J Physiol Renal Physiol, May 1, 2006; 290(5): F997 - F1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Ferrari, M. Ferrandi, G. Valentini, and G. Bianchi Rostafuroxin: an ouabain antagonist that corrects renal and vascular Na+-K+- ATPase alterations in ouabain and adducin-dependent hypertension Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2006; 290(3): R529 - R535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Plourde and S. P. Soltoff Ouabain potentiates the activation of ERK1/2 by carbachol in parotid gland epithelial cells; inhibition of ERK1/2 reduces Na+-K+-ATPase activity Am J Physiol Cell Physiol, March 1, 2006; 290(3): C702 - C710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Kennedy, S. Vetteth, S. M. Periyasamy, M. Kanj, L. Fedorova, S. Khouri, M. B. Kahaleh, Z. Xie, D. Malhotra, N. I. Kolodkin, et al. Central Role for the Cardiotonic Steroid Marinobufagenin in the Pathogenesis of Experimental Uremic Cardiomyopathy Hypertension, March 1, 2006; 47(3): 488 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Cantin, G. Bilodeau, C. Ouellet, J. Liao, and J. W. Hanrahan Oxidant stress suppresses CFTR expression Am J Physiol Cell Physiol, January 1, 2006; 290(1): C262 - C270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Tian, T. Cai, Z. Yuan, H. Wang, L. Liu, M. Haas, E. Maksimova, X.-Y. Huang, and Z.-J. Xie Binding of Src to Na+/K+-ATPase Forms a Functional Signaling Complex Mol. Biol. Cell, January 1, 2006; 17(1): 317 - 326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Wasserstrom and G. L. Aistrup Digitalis: new actions for an old drug Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H1781 - H1793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yuan, T. Cai, J. Tian, A. V. Ivanov, D. R. Giovannucci, and Z. Xie Na/K-ATPase Tethers Phospholipase C and IP3 Receptor into a Calcium-regulatory Complex Mol. Biol. Cell, September 1, 2005; 16(9): 4034 - 4045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Kaplan A Moving New Role for the Sodium Pump in Epithelial Cells and Carcinomas Sci. Signal., June 21, 2005; 2005(289): pe31 - pe31. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. K. Scheidenhelm, J. Cresswell, C. A. Haipek, T. P. Fleming, R. W. Mercer, and D. H. Gutmann Akt-Dependent Cell Size Regulation by the Adhesion Molecule on Glia Occurs Independently of Phosphatidylinositol 3-Kinase and Rheb Signaling Mol. Cell. Biol., April 15, 2005; 25(8): 3151 - 3162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kometiani, L. Liu, and A. Askari Digitalis-Induced Signaling by Na+/K+-ATPase in Human Breast Cancer Cells Mol. Pharmacol., March 1, 2005; 67(3): 929 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Dong, Q. Li, S.-c. Lyu, A. M. Krensky, and C. Clayberger A novel apoptosis pathway activated by the carboxyl terminus of p21 Blood, February 1, 2005; 105(3): 1187 - 1194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. Akimova, A. Y. Bagrov, O. D. Lopina, A. V. Kamernitsky, J. Tremblay, P. Hamet, and S. N. Orlov Cardiotonic Steroids Differentially Affect Intracellular Na+ and [Na+]i/[K+]i-independent Signaling in C7-MDCK Cells J. Biol. Chem., January 7, 2005; 280(1): 832 - 839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Y. Xu, E. Takimoto, G. J. Juang, Q. Zhang, H. Rohde, and A. C. Myers Evidence that the H1-H2 domain of {alpha}1 subunit of (Na++K+)-ATPase participates in the regulation of cardiac contraction FASEB J, January 1, 2005; 19(1): 53 - 61. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dworschak, D. Breukelmann, and J. D. Hannon The Impact of Isoflurane During Simulated Ischemia/Reoxygenation on Intracellular Calcium, Contractile Function, and Arrhythmia in Ventricular Myocytes Anesth. Analg., November 1, 2004; 99(5): 1302 - 1307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Barbato, S. Rashid, P. J. Mulrow, J. I. Shapiro, and R. Franco-Saenz Mechanisms for Aldosterone and Spironolactone-Induced Positive Inotropic Actions in the Rat Heart Hypertension, November 1, 2004; 44(5): 751 - 757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ferrandi, I. Molinari, P. Barassi, E. Minotti, G. Bianchi, and P. Ferrari Organ Hypertrophic Signaling within Caveolae Membrane Subdomains Triggered by Ouabain and Antagonized by PST 2238 J. Biol. Chem., August 6, 2004; 279(32): 33306 - 33314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, M. Haas, M. Liang, T. Cai, J. Tian, S. Li, and Z. Xie Ouabain Assembles Signaling Cascades through the Caveolar Na+/K+-ATPase J. Biol. Chem., April 23, 2004; 279(17): 17250 - 17259. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Reich, S. E. Mason, and B. E. Alger Novel Form of LTD Induced by Transient, Partial Inhibition of the Na,K-Pump in Rat Hippocampal CA1 Cells J Neurophysiol, January 1, 2004; 91(1): 239 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Abramowitz, C. Dai, K. K. Hirschi, R. I. Dmitrieva, P. A. Doris, L. Liu, and J. C. Allen Ouabain- and Marinobufagenin-Induced Proliferation of Human Umbilical Vein Smooth Muscle Cells and a Rat Vascular Smooth Muscle Cell Line, A7r5 Circulation, December 16, 2003; 108(24): 3048 - 3053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Liu and J. I. Shapiro Endocytosis and Signal Transduction: Basic Science Update Biol Res Nurs, October 1, 2003; 5(2): 117 - 128. [Abstract] [PDF]
|
|
|
|
|
|
Z. Xie and T. Cai Na+-K+-ATPase-Mediated Signal Transduction: From Protein Interaction to Cellular Function Mol. Interv., May 1, 2003; 3(3): 157 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Baudouin-Legros, F. Brouillard, D. Tondelier, A. Hinzpeter, and A. Edelman Effect of ouabain on CFTR gene expression in human Calu-3 cells Am J Physiol Cell Physiol, March 1, 2003; 284(3): C620 - C626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Kennedy, E. Omran, S. M. Periyasamy, J. Nadoor, A. Priyadarshi, J. C. Willey, D. Malhotra, Z. Xie, and J. I. Shapiro Effect of Chronic Renal Failure on Cardiac Contractile Function, Calcium Cycling, and Gene Expression of Proteins Important for Calcium Homeostasis in the Rat J. Am. Soc. Nephrol., January 1, 2003; 14(1): 90 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nishio, S. W. Ruch, and J. A. Wasserstrom Positive inotropic effects of ouabain in isolated cat ventricular myocytes in sodium-free conditions Am J Physiol Heart Circ Physiol, November 1, 2002; 283(5): H2045 - H2053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Taurin, N. O Dulin, D. Pchejetski, R. Grygorczyk, J. Tremblay, P. Hamet, and S. N Orlov c-Fos expression in ouabain-treated vascular smooth muscle cells from rat aorta: evidence for an intracellular-sodium-mediated, calcium-independent mechanism J. Physiol., September 15, 2002; 543(3): 835 - 847. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. I. Dmitrieva and P. A. Doris Cardiotonic Steroids: Potential Endogenous Sodium Pump Ligands with Diverse Function Experimental Biology and Medicine, September 1, 2002; 227(8): 561 - 569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kajikawa, S. Fujimoto, Y. Tsuura, E. Mukai, T. Takeda, Y. Hamamoto, M. Takehiro, J. Fujita, Y. Yamada, and Y. Seino Ouabain Suppresses Glucose-Induced Mitochondrial ATP Production and Insulin Release by Generating Reactive Oxygen Species in Pancreatic Islets Diabetes, August 1, 2002; 51(8): 2522 - 2529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Haas, H. Wang, J. Tian, and Z. Xie Src-mediated Inter-receptor Cross-talk between the Na+/K+-ATPase and the Epidermal Growth Factor Receptor Relays the Signal from Ouabain to Mitogen-activated Protein Kinases J. Biol. Chem., May 17, 2002; 277(21): 18694 - 18702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Rajasekaran, L. G. Palmer, S. Y. Moon, A. Peralta Soler, G. L. Apodaca, J. F. Harper, Y. Zheng, and A. K. Rajasekaran Na,K-ATPase Activity Is Required for Formation of Tight Junctions, Desmosomes, and Induction of Polarity in Epithelial Cells Mol. Biol. Cell, December 1, 2001; 12(12): 3717 - 3732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Aydemir-Koksoy, J. Abramowitz, and J. C. Allen Ouabain-induced Signaling and Vascular Smooth Muscle Cell Proliferation J. Biol. Chem., November 30, 2001; 276(49): 46605 - 46611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mohammadi, P. Kometiani, Z. Xie, and A. Askari Role of Protein Kinase C in the Signal Pathways That Link Na+/K+-ATPase to ERK1/2 J. Biol. Chem., November 2, 2001; 276(45): 42050 - 42056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tian, X. Gong, and Z. Xie Signal-transducing function of Na+-K+-ATPase is essential for ouabain's effect on [Ca2+]i in rat cardiac myocytes Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H1899 - H1907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Manzerra, M. M. Behrens, L. M. T. Canzoniero, X. Q. Wang, V. Heidinger, T. Ichinose, S. P. Yu, and D. W. Choi Zinc induces a Src family kinase-mediated up-regulation of NMDA receptor activity and excitotoxicity PNAS, September 25, 2001; 98(20): 11055 - 11061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Haas, A. Askari, and Z. Xie Involvement of Src and Epidermal Growth Factor Receptor in the Signal-transducing Function of Na+/K+-ATPase J. Biol. Chem., September 1, 2000; 275(36): 27832 - 27837. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |