Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M001280200 on May 19, 2000

J. Biol. Chem., Vol. 275, Issue 36, 28110-28119, September 8, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/36/28110    most recent
M001280200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Warner, G. J.
Right arrow Articles by Faust, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Warner, G. J.
Right arrow Articles by Faust, J. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Inhibition of Selenoprotein Synthesis by Selenocysteine tRNA[Ser]Sec Lacking Isopentenyladenosine*

Gregory J. WarnerDagger §, Marla J. Berry||, Mohamed E. Moustafa**, Bradley A. Carlson**, Dolph L. Hatfield**, and Jerry R. FaustDagger §§¶¶

From the Dagger  Tufts University School of Medicine, Department of Physiology, Boston, Massachusetts 02111, || Harvard Medical School, Thyroid Division, Department of Medicine, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115, and the ** Basic Research Laboratory, Division of Basic Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892

A common posttranscriptional modification of tRNA is the isopentenylation of adenosine at position 37, creating isopentenyladenosine (i6A). The role of this modified nucleoside in protein synthesis of higher eukaryotes is not well understood. Selenocysteyl (Sec) tRNA (tRNA[Ser]Sec) decodes specific UGA codons and contains i6A. To address the role of the modified nucleoside in this tRNA, we constructed a site-specific mutation, which eliminates the site of isopentenylation, in the Xenopus tRNA[Ser]Sec gene. Transfection of the mutant tRNA[Ser]Sec gene resulted in 80% and 95% reduction in the expression of co-transfected selenoprotein genes encoding type I and II iodothyronine deiodinases, respectively. A similar decrease in type I deiodinase synthesis was observed when transfected cells were treated with lovastatin, an inhibitor of the biosynthesis of the isopentenyl moiety. Neither co-transfection with the mutant tRNA gene nor lovastatin treatment reduced type I deiodinase mRNA levels. Also, mutant tRNA expression did not alter initiation of translation or degradation of the type I deiodinase protein. Furthermore, isopentenylation of tRNA[Ser]Sec was not required for synthesis of Sec on the tRNA. We conclude that isopentenylation of tRNA[Ser]Sec is required for efficient translational decoding of UGA and synthesis of selenoproteins.

* This work was supported by Grant MCB-9316131 from the National Science Foundation (to J. R. F.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Present address: Enanta Pharmaceuticals, 750 Main St., Cambridge, MA, 02139.

Supported by National Institutes of Health Training Grant T32 DK07542.

§§ To whom correspondence should be addressed: Tufts University School of Medicine, Dept. of Physiology, 136 Harrison Ave., Boston, MA 02111. Tel.: 617-636-2405; Fax: 617-636-0445; E-mail: jerry.faust@ tufts.edu.

¶¶ Established Investigator of the American Heart Association.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
A. Kromer and B. Moosmann
Statin-Induced Liver Injury Involves Cross-Talk between Cholesterol and Selenoprotein Biosynthetic Pathways
Mol. Pharmacol., June 1, 2009; 75(6): 1421 - 1429.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Suzuki, V. P. Kelly, H. Motohashi, O. Nakajima, S. Takahashi, S. Nishimura, and M. Yamamoto
Deletion of the Selenocysteine tRNA Gene in Macrophages and Liver Results in Compensatory Gene Induction of Cytoprotective Enzymes by Nrf2
J. Biol. Chem., January 25, 2008; 283(4): 2021 - 2030.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
P. van der Harst, A. A. Voors, W. H. van Gilst, M. Bohm, and D. J. van Veldhuisen
Statins in the treatment of chronic heart failure: Biological and clinical considerations
Cardiovasc Res, August 1, 2006; 71(3): 443 - 454.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
J. Kohrle, F. Jakob, B. Contempre, and J. E. Dumont
Selenium, the Thyroid, and the Endocrine System
Endocr. Rev., December 1, 2005; 26(7): 944 - 984.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
R. R. JAMESON and A. M. DIAMOND
A regulatory role for Sec tRNA[Ser]Sec in selenoprotein synthesis
RNA, July 1, 2004; 10(7): 1142 - 1152.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
U. Schweizer, L. Schomburg, and N. E. Savaskan
The Neurobiology of Selenium: Lessons from Transgenic Mice
J. Nutr., April 1, 2004; 134(4): 707 - 710.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
A. K. Hopper and E. M. Phizicky
tRNA transfers to the limelight
Genes & Dev., January 15, 2003; 17(2): 162 - 180.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement