HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 275, Issue 36, 28276-28284, September 8, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/36/28276    most recent M003734200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nakamura, N. Articles by Hirose, S. Search for Related Content PubMed PubMed Citation Articles by Nakamura, N. Articles by Hirose, S.

Complex Structure and Regulation of Expression of the Rat Gene for Inward Rectifier Potassium Channel Kir7.1^*

Nobuhiro Nakamura, Yoshiro Suzuki, Yugo Ikeda, Michitaka Notoya, and Shigehisa Hirose

From the Department of Biological Sciences, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan

Genomic organization of the rat inward rectifier K⁺ channel Kir7.1 was determined in an attempt to clarify how multiple species of its mRNA are generated in a tissue-specific manner and how its expression is regulated. The rat Kir7.1 gene spans >40 kilobases (kb) and consists of eight exons; the first four exons encode the 5'-untranslated region that is unusually long (~3 kb). The coding region is located in exons 5 and 6. In the testis, exon 4 is processed as four exons (4a-4d), whereas it is recognized as a single exon in the small intestine. The three major species of rat Kir7.1 mRNA (1.4, 2.2, and 3.2 kb) were found to arise from alternative usage of the two promoters and polyadenylation signals and by alternative splicing of the 5'-noncoding exons. The splicing pattern of the 5'-noncoding exons is quite complex and highly tissue-specific, suggesting that complex mechanisms may operate to regulate the Kir7.1 expression. Deletion and mutational analysis of the promoter activity indicated that the rat Kir7.1 gene is regulated by cAMP through a CCAAT element. The cAMP induction was also demonstrated using the rat follicular cell line FRTL-5 endogenously expressing Kir7.1.

The nucleotide sequences reported in this paper have been submitted to the DDBJ/GenBank^TM/EBI Data Bank with accession numbers AB034241 and AB034242.

This article has been cited by other articles:

				N. Nakamura, H. Fukuda, A. Kato, and S. Hirose MARCH-II Is a Syntaxin-6-binding Protein Involved in Endosomal Trafficking Mol. Biol. Cell, April 1, 2005; 16(4): 1696 - 1710. [Abstract] [Full Text] [PDF]

				D. Yang, A. Pan, A. Swaminathan, G. Kumar, and B. A. Hughes Expression and Localization of the Inwardly Rectifying Potassium Channel Kir7.1 in Native Bovine Retinal Pigment Epithelium Invest. Ophthalmol. Vis. Sci., July 1, 2003; 44(7): 3178 - 3185. [Abstract] [Full Text] [PDF]