JBC Ideal method for primary cell transfection

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M004323200 on July 5, 2000

J. Biol. Chem., Vol. 275, Issue 37, 28507-28512, September 15, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/37/28507    most recent
M004323200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Puig-Kröger, A.
Right arrow Articles by Corbí, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Puig-Kröger, A.
Right arrow Articles by Corbí, A. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Polyomavirus Enhancer-binding Protein 2/Core Binding Factor/Acute Myeloid Leukemia Factors Contribute to the Cell Type-specific Activity of the CD11a Integrin Gene Promoter*

Amaya Puig-KrögerDagger §, Cristina López-RodríguezDagger §, Miguel RellosoDagger ||, Tilman Sánchez-ElsnerDagger ||, Arsenio NuedaDagger **, Eduardo MuñozDagger Dagger , Carmelo BernabéuDagger , and Angel L. CorbíDagger §§

From the Dagger  Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid 28006, Spain and the Dagger Dagger  Departamento de Inmunología, Facultad de Medicina, Universidad de Córdoba, Córdoba, 14071 Spain

The CD11a/CD18 leukocyte integrin (LFA-1; also known as alpha L/beta 2) mediates leukocyte transendothelial migration during immune and inflammatory responses and participates in lymphoma metastasis. CD11a/CD18 leukocyte-restricted expression is controlled by the CD11a gene promoter, which confers tissue-specific expression to reporter genes in vitro and in vivo. DNase I protection analysis of the CD11a proximal gene promoter revealed DNA-protein interactions centered at position -110 (CD11a-110). Disruption of CD11a-110 reduced CD11a promoter activity in a cell type-specific manner, as it reduced its activity by 70% in Jurkat lymphoid cells, whereas the effect was considerably lower in K562 and HepG2 cells. Electrophoretic mobility shift assays showed evidence of cell type-specific differences in CD11a-110 binding and indicated its specific recognition by members of the polyomavirus enhancer-binding protein 2/core binding factor (CBF)/acute myeloid leukemia (AML) family of transcription factors. AML1B/CBFbeta transactivated the CD11a promoter, with AML1B/CBFbeta -mediated transactivation being completely dependent on the integrity of the CD11a-110 element. Therefore, CBF/AML factors play a role in the cell type-restricted transcription of the CD11a integrin gene through recognition of CD11a-110. The involvement of CBF/AML factors in CD11a expression raises the possibility that CD11a/CD18 expression might be deregulated in acute myeloid and B-lineage acute lymphoblastic leukemias, thus contributing to their altered adhesion and metastatic potential.

* This work was supported by Comision Interministerial de Cienca Y Tecnologia (CICYT) Grant SAF98/0068 and Comunidad Autónoma de Madrid Grant 08.2/0035.1/99 (to A. L. C.) and by CICYT Grant SAF97/0034 (to C. B.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ These authors contributed equally to this work and the order of authorship is arbitrary.

Current address: Center for Blood Research, Harvard Medical School, 200 Longwood Ave., Boston MA 02115.

|| Recipients of predoctoral and postdoctoral fellowships from Comunidad Autónoma de Madrid.

** Current address: Centro de Investigacion, Almirall Prodesfarma, S.A., Cardener 68-74, Barcelona 08024, Spain.

§§ To whom correspondence should be addressed. Tel.: 34-91-5644562, ext. 4312; Fax: 34-91-5627518; E-mail: acorbi@cib.csic.es.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
H.-I. Trompeter, N. Gomez-Lozano, S. Santourlidis, B. Eisermann, P. Wernet, C. Vilches, and M. Uhrberg
Three Structurally and Functionally Divergent Kinds of Promoters Regulate Expression of Clonally Distributed Killer Cell Ig-Like Receptors (KIR), of KIR2DL4, and of KIR3DL3
J. Immunol., April 1, 2005; 174(7): 4135 - 4143.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Planaguma, M. Diaz-Fuertes, A. Gil-Moreno, M. Abal, M. Monge, A. Garcia, T. Baro, T. M. Thomson, J. Xercavins, F. Alameda, et al.
A Differential Gene Expression Profile Reveals Overexpression of RUNX1/AML1 in Invasive Endometrioid Carcinoma
Cancer Res., December 15, 2004; 64(24): 8846 - 8853.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. W. Lee, H. Y. Chung, L. A. Ehrlich, D. F. Jelinek, N. S. Callander, G. D. Roodman, and S. J. Choi
IL-3 expression by myeloma cells increases both osteoclast formation and growth of myeloma cells
Blood, March 15, 2004; 103(6): 2308 - 2315.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Puig-Kroger, T. Sanchez-Elsner, N. Ruiz, E. J. Andreu, F. Prosper, U. B. Jensen, J. Gil, P. Erickson, H. Drabkin, Y. Groner, et al.
RUNX/AML and C/EBP factors regulate CD11a integrin expression in myeloid cells through overlapping regulatory elements
Blood, November 1, 2003; 102(9): 3252 - 3261.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Puig-Kroger, O. Muniz-Pello, R. Selgas, G. Criado, M-A. Bajo, J. A. Sanchez-Tomero, V. Alvarez, G. del Peso, P. Sanchez-Mateos, C. Holmes, et al.
Peritoneal dialysis solutions inhibit the differentiation and maturation of human monocyte-derived dendritic cells: effect of lactate and glucose-degradation products
J. Leukoc. Biol., April 1, 2003; 73(4): 482 - 492.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. G. de Guzman, A. J. Warren, Z. Zhang, L. Gartland, P. Erickson, H. Drabkin, S. W. Hiebert, and C. A. Klug
Hematopoietic Stem Cell Expansion and Distinct Myeloid Developmental Abnormalities in a Murine Model of the AML1-ETO Translocation
Mol. Cell. Biol., August 1, 2002; 22(15): 5506 - 5517.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.