|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 37, 28569-28574, September 15, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Friedrich-Miescher Institut, CH-4058 Basel,
Switzerland
Properdin is the positive regulator of the
alternative pathway of complement activation. The 53-kDa protein is
essentially composed of six thrombospondin type 1 repeats, all
of which contain the WXXW motif, the recognition
sequence for C-mannosylation. C-Mannosylation
is a post-translational modification of tryptophan residues in which,
in contrast to the well known N- and
O-glycosylation, the carbohydrate is attached via a C-C
bond to C-2 of the indole moiety of tryptophan.
C-Mannosylation was first found in human RNase 2 and
interleukin-12. The terminal complement proteins C6-C9 also carry this
modification as part of their thrombospondin type 1 repeats. We studied
the C-mannosylation pattern of human properdin by mass
spectrometry and Edman degradation. Properdin contains 20 tryptophans
of which 17 are part of a WXXW motif. Fourteen tryptophans
were found to be modified 100%. This is the first example of a protein
in which the majority of tryptophan residues occurs in the
C-mannosylated form. These results show that
C-mannosylated proteins occur at several steps along the
complement activation cascade. Therefore, this system would be ideal to
investigate the function of C-mannosylation.
Properdin, the Positive Regulator of Complement, Is Highly
C-Mannosylated*
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Friedrich-Miescher
Institut, Maulbeerstr. 66, CH-4058 Basel, Switzerland. Tel.: 41-61-697-4531; Fax: 41-61-697-3976; E-mail:
Jan.Hofsteenge@fmi.ch.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Hess, J. J. Keusch, S. A. L. Oberstein, R. C. M. Hennekam, and J. Hofsteenge Peters Plus Syndrome Is a New Congenital Disorder of Glycosylation and Involves Defective O-Glycosylation of Thrombospondin Type 1 Repeats J. Biol. Chem., March 21, 2008; 283(12): 7354 - 7360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Muroi, S. Manabe, M. Ikezaki, Y. Urata, S. Sato, T. Kondo, Y. Ito, and Y. Ihara C-Mannosylated peptides derived from the thrombospondin type 1 repeat enhance lipopolysaccharide-induced signaling in macrophage-like RAW264.7 cells Glycobiology, September 1, 2007; 17(9): 1015 - 1028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Julenius NetCGlyc 1.0: prediction of mammalian C-mannosylation sites Glycobiology, August 1, 2007; 17(8): 868 - 876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Li, L. Cui, D. L. Rock, and J. Li Novel Glycosidic Linkage in Aedes aegypti Chorion Peroxidase: N-MANNOSYL TRYPTOPHAN J. Biol. Chem., November 18, 2005; 280(46): 38513 - 38521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ihara, S. Manabe, M. Kanda, H. Kawano, T. Nakayama, I. Sekine, T. Kondo, and Y. Ito Increased expression of protein C-mannosylation in the aortic vessels of diabetic Zucker rats Glycobiology, April 1, 2005; 15(4): 383 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tan, M. Duquette, J.-h. Liu, Y. Dong, R. Zhang, A. Joachimiak, J. Lawler, and J.-h. Wang Crystal structure of the TSP-1 type 1 repeats: a novel layered fold and its biological implication J. Cell Biol., October 28, 2002; 159(2): 373 - 382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Spiro Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds Glycobiology, April 1, 2002; 12(4): 43R - 56R. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gonzalez de Peredo, D. Klein, B. Macek, D. Hess, J. Peter-Katalinic, and J. Hofsteenge C-Mannosylation and O-Fucosylation of Thrombospondin Type 1 Repeats Mol. Cell. Proteomics, January 1, 2002; 1(1): 11 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Anand, J. S. Rush, S. Ray, M.-A. Doucey, J. Weik, F. E. Ware, J. Hofsteenge, C. J. Waechter, and M. A. Lehrman Requirement of the Lec35 Gene for All Known Classes of Monosaccharide-P-Dolichol-dependent Glycosyltransferase Reactions in Mammals Mol. Biol. Cell, February 1, 2001; 12(2): 487 - 501. [Abstract] [Full Text]
|
|
|
|
|
|
J. Hofsteenge, K. G. Huwiler, B. Macek, D. Hess, J. Lawler, D. F. Mosher, and J. Peter-Katalinic C-Mannosylation and O-Fucosylation of the Thrombospondin Type 1 Module J. Biol. Chem., February 23, 2001; 276(9): 6485 - 6498. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |