|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 37, 28834-28842, September 15, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Estrogen actions in target organs are normally
mediated via activation of nuclear estrogen receptors (ERs). By using
mRNA differential display technique, we show, herein, that
estradiol-17
Estrogen Targets Genes Involved in Protein Processing, Calcium
Homeostasis, and Wnt Signaling in the Mouse Uterus Independent of
Estrogen Receptor-
and -
*
§¶,,,
, and Department of Obstetrics & Gynecology, the
§ Department of Molecular & Integrative Physiology, and
the Department of Pediatrics, Ralph L. Smith Research Center,
University of Kansas Medical Center, Kansas City, Kansas 66160
(E2) and its catechol metabolite
4-hydroxy-E2 (4OHE2) can modulate uterine gene
expression in ER
(
/) mice. Whereas administration of
E2 or 4OHE2 rapidly up-regulated (4-8-fold)
the expression of immunoglobulin heavy chain binding protein
(Bip), calpactin I (CalP),
calmodulin (CalM), and Sik similar protein
(Sik-SP) genes in ovariectomized wild-type or ER(/)
mice, the expression of secreted frizzled related protein-2
(SFRP-2) gene was down-regulated (4-fold). Bip, CalP, and
CalM are calcium-binding proteins and implicated in calcium
homeostasis, whereas SFRP-2 is a negative regulator of Wnt signaling.
Bip and Sik-SP also possess chaperone-like functions. Administration of
ICI-182,780 or cycloheximide failed to influence these estrogenic
responses, demonstrating that these effects occur independent of ER,
ER, or protein synthesis. In situ hybridization showed
differential cell-specific expression of these genes in wild-type and
ER(/) uteri. Although progesterone can antagonize or synergize
estrogen actions, it had minimal effects on these estrogenic responses.
Collectively, the results demonstrate that estrogens have a unique
ability to influence specific genes in the uterus not involving
classical nuclear ERs.
*
This work was supported in part by National Institutes of
Health Grants ES-07814 (to S. K. Das), HD-12304, and HD-29968 (to S. K. Dey).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Otto, I. Fuchs, G. Kauselmann, H. Kern, B. Zevnik, P. Andreasen, G. Schwarz, H. Altmann, M. Klewer, M. Schoor, et al. GPR30 Does Not Mediate Estrogenic Responses in Reproductive Organs in Mice Biol Reprod, January 1, 2009; 80(1): 34 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ray, F. Xu, H. Wang, and S. K. Das Cooperative Control via Lymphoid Enhancer Factor 1/T Cell Factor 3 and Estrogen Receptor-{alpha} for Uterine Gene Regulation by Estrogen Mol. Endocrinol., May 1, 2008; 22(5): 1125 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Roepke, A. Malyala, M. A. Bosch, M. J. Kelly, and O. K. Ronnekleiv Estrogen Regulation of Genes Important for K+ Channel Signaling in the Arcuate Nucleus Endocrinology, October 1, 2007; 148(10): 4937 - 4951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ray, F. Xu, P. Li, N. S. Sanchez, H. Wang, and S. K. Das Increased Level of Cellular Bip Critically Determines Estrogenic Potency for a Xenoestrogen Kepone in the Mouse Uterus Endocrinology, October 1, 2007; 148(10): 4774 - 4785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ray, X. Hou, H.-E. Zhou, H. Wang, and S. K. Das Bip Is a Molecular Link between the Phase I and Phase II Estrogenic Responses in Uterus Mol. Endocrinol., August 1, 2006; 20(8): 1825 - 1837. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Han, J. S. Heo, and Y. J. Lee Estradiol-17beta stimulates proliferation of mouse embryonic stem cells: involvement of MAPKs and CDKs as well as protooncogenes Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1067 - C1075. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Li, Z. Li, P. M. Howley, and D. B. Sacks E6AP and Calmodulin Reciprocally Regulate Estrogen Receptor Stability J. Biol. Chem., January 27, 2006; 281(4): 1978 - 1985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, S. Tranguch, H. Xie, G. Hanley, S. K. Das, and S. K. Dey Variation in commercial rodent diets induces disparate molecular and physiological changes in the mouse uterus PNAS, July 12, 2005; 102(28): 9960 - 9965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Carson Uterine sensing of the embryo PNAS, June 14, 2005; 102(24): 8397 - 8398. [Full Text] [PDF]
|
|
|
|
|
|
X. Hou, Y. Tan, M. Li, S. K. Dey, and S. K. Das Canonical Wnt Signaling Is Critical to Estrogen-Mediated Uterine Growth Mol. Endocrinol., December 1, 2004; 18(12): 3035 - 3049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. Mohamed, D. Dufort, and H. J. Clarke Expression and Estradiol Regulation of Wnt Genes in the Mouse Blastocyst Identify a Candidate Pathway for Embryo-Maternal Signaling at Implantation Biol Reprod, August 1, 2004; 71(2): 417 - 424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Dey, H. Lim, S. K. Das, J. Reese, B. C. Paria, T. Daikoku, and H. Wang Molecular Cues to Implantation Endocr. Rev., June 1, 2004; 25(3): 341 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Lee, C.-T. Lin, L.-L. Chueh, and C.-J. Chang Autocrine/Paracrine Secreted Frizzled-related Protein 2 Induces Cellular Resistance to Apoptosis: A POSSIBLE MECHANISM OF MAMMARY TUMORIGENESIS J. Biol. Chem., April 9, 2004; 279(15): 14602 - 14609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Politi, M. Szabolcs, P. Fisher, A. Kljuic, T. Ludwig, and A. Efstratiadis A Mouse Model of Uterine Leiomyosarcoma Am. J. Pathol., January 1, 2004; 164(1): 325 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Bigsby, A. Caperell-Grant, N. Berry, K. Nephew, and D. Lubahn Estrogen Induces a Systemic Growth Factor Through an Estrogen Receptor-Alpha-Dependent Mechanism Biol Reprod, January 1, 2004; 70(1): 178 - 183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Rehman, S. Yin, B. A. Mayhew, R.A. Word, and W. E. Rainey Human myometrial adaptation to pregnancy: cDNA microarray gene expression profiling of myometrium from non-pregnant and pregnant women Mol. Hum. Reprod., November 1, 2003; 9(11): 681 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Fertuck, J. E. Eckel, C. Gennings, and T. R. Zacharewski Identification of temporal patterns of gene expression in the uteri of immature, ovariectomized mice following exposure to ethynylestradiol Physiol Genomics, October 17, 2003; 15(2): 127 - 141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wu, S.-T. Pang, L. Sahlin, A. Blanck, G. Norstedt, and A. Flores-Morales Gene Expression Profiling of the Effects of Castration and Estrogen Treatment in the Rat Uterus Biol Reprod, October 1, 2003; 69(4): 1308 - 1317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Daikoku, H. Matsumoto, R. A. Gupta, S. K. Das, M. Gassmann, R. N. DuBois, and S. K. Dey Expression of Hypoxia-inducible Factors in the Peri-implantation Mouse Uterus Is Regulated in a Cell-specific and Ovarian Steroid Hormone-dependent Manner. EVIDENCE FOR DIFFERENTIAL FUNCTION OF HIFs DURING EARLY PREGNANCY J. Biol. Chem., February 21, 2003; 278(9): 7683 - 7691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Carson, E. Lagow, A. Thathiah, R. Al-Shami, M. C. Farach-Carson, M. Vernon, L. Yuan, M. A. Fritz, and B. Lessey Changes in gene expression during the early to mid-luteal (receptive phase) transition in human endometrium detected by high-density microarray screening Mol. Hum. Reprod., September 1, 2002; 8(9): 871 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Corey, J. E. Quinn, M. J. Emond, K. R. Buhler, L. G. Brown, and R. L. Vessella Inhibition of Androgen-independent Growth of Prostate Cancer Xenografts by 17{beta}-Estradiol Clin. Cancer Res., April 1, 2002; 8(4): 1003 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reese, S. K. Das, B. C. Paria, H. Lim, H. Song, H. Matsumoto, K. L. Knudtson, R. N. DuBois, and S. K. Dey Global Gene Expression Analysis to Identify Molecular Markers of Uterine Receptivity and Embryo Implantation J. Biol. Chem., November 16, 2001; 276(47): 44137 - 44145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.'i Nakamoto, A. Ito, K. Watabe, Y.-i. Koma, H. Asada, K. Yoshikawa, Y. Shinomura, Y. Matsuzawa, H. Nojima, and Y. Kitamura Increased Expression of a Nucleolar Nop5/Sik Family Member in Metastatic Melanoma Cells : Evidence for Its Role in Nucleolar Sizing and Function Am. J. Pathol., October 1, 2001; 159(4): 1363 - 1374. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |