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Originally published In Press as doi:10.1074/jbc.M002175200 on June 23, 2000

J. Biol. Chem., Vol. 275, Issue 37, 28882-28887, September 15, 2000
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The POU Domain Transcription Factor Tst-1 Activates Somatostatin Receptor 1 Gene Expression in Pancreatic beta -Cells*

Hans Baumeister and Wolfgang MeyerhofDagger

From the Abteilung Molekulare Genetik, Deutsches Institut für Ernährungsforschung und Universität Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Potsdam-Rehbrücke, Germany

The peptide hormone somatostatin inhibits the release of insulin. The gene encoding somatostatin receptor 1 is expressed in pancreatic beta -cells and insulinoma RIN 1046-38 cells. In the present study the mechanisms underlying the regulation of the somatostatin receptor 1 gene in pancreatic beta -cells were investigated. Transient transfections of RIN 1046-38 cells with promoter/reporter gene constructs and footprint analysis revealed two regions, fp1 and fp2, that were necessary for the observed promoter activity. Mutagenesis of the fp2 region delineated the cis-acting element to the motif 5'-TTAATCATT-3'. The POU domain transcription factor Tst-1 was identified as trans-activator mediating the 5'-TTAATCATT-3' motif-dependent transcription in RIN 1046-38 cells and heterologous CV1 cells. Tst-1, known as a transcriptional regulator in keratinocytes, glial cells, and neurons, has been detected by immunohistochemistry in pancreatic islets. Altogether, we demonstrate Tst-1 as transcriptional regulator in pancreatic neuroendocrine cells.

* This work was supported by the Fonds der Chemischen Industrie (to W. M.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Abteilung Molekulare Genetik, Deutsches Institut für Ernährungsforschung, Arthur-Scheunert-Allee 114-116, D-14558 Potsdam-Rehbrücke, Germany. Tel.: 49 33200 88 282; Fax: 49 33200 88 444; E-mail: meyerhof@www.dife.de.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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This article has been cited by other articles:


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W. W. Woodmansee, R. L. Mouser, D. F. Gordon, J. M. Dowding, W. M. Wood, and E. C. Ridgway
Mutational Analysis of the Mouse Somatostatin Receptor Type 5 Gene Promoter
Endocrinology, June 1, 2002; 143(6): 2268 - 2276.
[Abstract] [Full Text] [PDF]


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