|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 37, 28893-28901, September 15, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the The ability of Shigella to mediate
actin-based motility within the host cell is a prominent pathogenic
feature of bacillary dysentery. The ability is dependent on the
interaction of VirG with neural Wiskott-Aldrich syndrome protein
(N-WASP), which in turn mediates recruitment of Arp2/3 complex and
several actin-related proteins. In the present study, we show that
profilin I is essential to the rapid movement of Shigella
in epithelial cells, for which the capacity of profilin to interact
with G-actin and N-WASP is critical. In COS-7 cells overexpressing
either mutated profilin H119E, which failed to bind G-actin, or H133S,
which is unable to interact with poly-L-proline,
Shigella motility was significantly inhibited. Similarly,
depletion of profilin from Xenopus egg extracts resulted in
a decrease in bacterial motility that was completely rescued by adding
back profilin I but not H119E or H133S. In COS-7 cells overexpressing a
N-WASP mutant lacking the proline-rich domain (
Profilin Is Required for Sustaining Efficient Intra- and
Intercellular Spreading of Shigella flexneri*
,,¶
Division of Bacterial Infection, Department
of Microbiology and Immunology, § Department of
Biochemistry, Institute of Medical Science, University of Tokyo,
Minato-ku, Tokyo 108-8639 and the ¶ Department of Bacterial
Toxicology, Research Institute for Microbial Diseases, Osaka
University, Suita, Osaka 565-0871, Japan
p) unable to interact
with profilin, the actin tail formation of intracellular
Shigella was inhibited. In N-WASP-depleted extracts, addition of p but not full-length N-WASP was unable to restore the
bacterial motility. Furthermore, in a plaque formation assay with
Madin-Darby canine kidney cell monolayers infected by
Shigella, Madin-Darby canine kidney cells stably expressing
H119E, H133S, or p reduced the bacterial cell-to-cell spreading.
These results indicate that profilin I associated with N-WASP is an
essential host factor for sustaining efficient intra- and intercellular spreading of Shigella.
*
This work was supported by the "Research for the Future"
Program of the Japan Society for the Promotion of Science, and a grant-in-aid for Scientific Research from the Japanese Ministry of
Education, Science, Sports and Culture.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan. Tel.: 81-3-5449-5252; Fax:
81-3-5449-5405; E-mail: sasakawa@ims.u-tokyo.ac.jp.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  O. C. Ezezika, N. S. Younger, J. Lu, D. A. Kaiser, Z. A. Corbin, B. J. Nolen, D. R. Kovar, and T. D. Pollard Incompatibility with Formin Cdc12p Prevents Human Profilin from Substituting for Fission Yeast Profilin: INSIGHTS FROM CRYSTAL STRUCTURES OF FISSION YEAST PROFILIN J. Biol. Chem., January 23, 2009; 284(4): 2088 - 2097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Syriani, A. Gomez-Cabrero, M. Bosch, A. Moya, E. Abad, A. Gual, X. Gasull, and M. Morales Profilin induces lamellipodia by growth factor-independent mechanism FASEB J, May 1, 2008; 22(5): 1581 - 1596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hanajima-Ozawa, T. Matsuzawa, A. Fukui, S. Kamitani, H. Ohnishi, A. Abe, Y. Horiguchi, and M. Miyake Enteropathogenic Escherichia coli, Shigella flexneri, and Listeria monocytogenes Recruit a Junctional Protein, Zonula Occludens-1, to Actin Tails and Pedestals Infect. Immun., February 1, 2007; 75(2): 565 - 573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Ding, A. Lambrechts, M. Parepally, and P. Roy Silencing profilin-1 inhibits endothelial cell proliferation, migration and cord morphogenesis J. Cell Sci., October 1, 2006; 119(19): 4127 - 4137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Burton, T. N. Oliver, and A. M. Pendergast Abl Kinases Regulate Actin Comet Tail Elongation via an N-WASP-Dependent Pathway Mol. Cell. Biol., October 15, 2005; 25(20): 8834 - 8843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Oda, H. Miki, I. Wada, H. Yamaguchi, D. Yamazaki, S. Suetsugu, M. Nakajima, A. Nakayama, K. Okawa, H. Miyazaki, et al. WAVE/Scars in platelets Blood, April 15, 2005; 105(8): 3141 - 3148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Harlander, M. Way, Q. Ren, D. Howe, S. S. Grieshaber, and R. A. Heinzen Effects of Ectopically Expressed Neuronal Wiskott-Aldrich Syndrome Protein Domains on Rickettsia rickettsii Actin-Based Motility Infect. Immun., March 1, 2003; 71(3): 1551 - 1556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Yarar, J. A. D'Alessio, R. L. Jeng, and M. D. Welch Motility Determinants in WASP Family Proteins Mol. Biol. Cell, November 1, 2002; 13(11): 4045 - 4059. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-Z. Idrissi, B. L. Wolf, and M. I. Geli Cofilin, But Not Profilin, Is Required for Myosin-I-Induced Actin Polymerization and the Endocytic Uptake in Yeast Mol. Biol. Cell, November 1, 2002; 13(11): 4074 - 4087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Benesch, S. Lommel, A. Steffen, T. E. B. Stradal, N. Scaplehorn, M. Way, J. Wehland, and K. Rottner Phosphatidylinositol 4,5-Biphosphate (PIP2)-induced Vesicle Movement Depends on N-WASP and Involves Nck, WIP, and Grb2 J. Biol. Chem., September 27, 2002; 277(40): 37771 - 37776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Goldberg Actin-Based Motility of Intracellular Microbial Pathogens Microbiol. Mol. Biol. Rev., December 1, 2001; 65(4): 595 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Suzuki and C. Sasakawa Molecular Basis of the Intracellular Spreading of Shigella Infect. Immun., October 1, 2001; 69(10): 5959 - 5966. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |