HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 275, Issue 37, 29076-29081, September 15, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/37/29076    most recent M003980200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hamada, M. Articles by Maraia, R. J. Search for Related Content PubMed PubMed Citation Articles by Hamada, M. Articles by Maraia, R. J. Social Bookmarking                      What's this?

Transcription Termination by RNA Polymerase III in Fission Yeast
A GENETIC AND BIOCHEMICALLY TRACTABLE MODEL SYSTEM^*

Mitsuhiro Hamada, Amy L. Sakulich, Shashi B. Koduru, and Richard J. Maraia^§

From the Laboratory of Molecular Growth Regulation, NICHHD, National Institutes of Health, Bethesda, Maryland 20892-2753

In order for RNA polymerase (pol) III to produce a sufficient quantity of RNAs of appropriate structure, initiation, termination, and reinitiation must be accurate and efficient. Termination-associated factors have been shown to facilitate reinitiation and regulate transcription in some species. Suppressor tRNA genes that differ in the dT(n) termination signal were examined for function in Schizosaccharomyces pombe. We also developed an S. pombe extract that is active for tRNA transcription that is described here for the first time. The ability of this tRNA gene to be transcribed in extracts from different species allowed us to compare termination in three model systems. Although human pol III terminates efficiently at 4 dTs and S. pombe at 5 dTs, Saccharomyces cerevisiae pol III requires 6 dTs to direct comparable but lower termination efficiency and also appears qualitatively distinct. Interestingly, this pattern of sensitivity to a minimal dT(n) termination signal was found to correlate with the sensitivity to -amanitin, as S. pombe was intermediate between human and S. cerevisiae pols III. The results establish that the pols III of S. cerevisiae, S. pombe, and human exhibit distinctive properties and that termination occurs in S. pombe in a manner that is functionally more similar to human than is S. cerevisiae.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				A. Soma, A. Onodera, J. Sugahara, A. Kanai, N. Yachie, M. Tomita, F. Kawamura, and Y. Sekine Permuted tRNA Genes Expressed via a Circular RNA Intermediate in Cyanidioschyzon merolae Science, October 19, 2007; 318(5849): 450 - 453. [Abstract] [Full Text] [PDF]

				M. A. Bayfield, T. E. Kaiser, R. V. Intine, and R. J. Maraia Conservation of a Masked Nuclear Export Activity of La Proteins and Its Effects on tRNA Maturation Mol. Cell. Biol., May 1, 2007; 27(9): 3303 - 3312. [Abstract] [Full Text] [PDF]

				C. A. S. Banks, S. E. Kong, H. Spahr, L. Florens, S. Martin-Brown, M. P. Washburn, J. W. Conaway, A. Mushegian, and R. C. Conaway Identification and Characterization of a Schizosaccharomyces pombe RNA Polymerase II Elongation Factor with Similarity to the Metazoan Transcription Factor ELL J. Biol. Chem., February 23, 2007; 282(8): 5761 - 5769. [Abstract] [Full Text] [PDF]

				H. Kato, D. B. Goto, R. A. Martienssen, T. Urano, K. Furukawa, and Y. Murakami RNA Polymerase II Is Required for RNAi-Dependent Heterochromatin Assembly Science, July 15, 2005; 309(5733): 467 - 469. [Abstract] [Full Text] [PDF]

				P. Braglia, R. Percudani, and G. Dieci Sequence Context Effects on Oligo(dT) Termination Signal Recognition by Saccharomyces cerevisiae RNA Polymerase III J. Biol. Chem., May 20, 2005; 280(20): 19551 - 19562. [Abstract] [Full Text] [PDF]

				Y. Huang, R. V. Intine, A. Mozlin, S. Hasson, and R. J. Maraia Mutations in the RNA Polymerase III Subunit Rpc11p That Decrease RNA 3' Cleavage Activity Increase 3'-Terminal Oligo(U) Length and La-Dependent tRNA Processing Mol. Cell. Biol., January 15, 2005; 25(2): 621 - 636. [Abstract] [Full Text] [PDF]

				C. Kohrer, E. L. Sullivan, and U. L. RajBhandary Complete set of orthogonal 21st aminoacyl-tRNA synthetase-amber, ochre and opal suppressor tRNA pairs: concomitant suppression of three different termination codons in an mRNA in mammalian cells Nucleic Acids Res., December 1, 2004; 32(21): 6200 - 6211. [Abstract] [Full Text] [PDF]

				Y. Huang, E. McGillicuddy, M. Weindel, S. Dong, and R. J. Maraia The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitment Nucleic Acids Res., April 15, 2003; 31(8): 2108 - 2116. [Abstract] [Full Text] [PDF]

				D. B. Schulman and D. R. Setzer Identification and characterization of transcription factor IIIA from Schizosaccharomyces pombe Nucleic Acids Res., July 1, 2002; 30(13): 2772 - 2781. [Abstract] [Full Text] [PDF]

				M. Hamada, Y. Huang, T. M. Lowe, and R. J. Maraia Widespread Use of TATA Elements in the Core Promoters for RNA Polymerases III, II, and I in Fission Yeast Mol. Cell. Biol., October 15, 2001; 21(20): 6870 - 6881. [Abstract] [Full Text] [PDF]

				Y. Huang and R. J. Maraia Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human Nucleic Acids Res., July 1, 2001; 29(13): 2675 - 2690. [Abstract] [Full Text] [PDF]

				Y. Huang, M. Hamada, and R. J. Maraia Isolation and Cloning of Four Subunits of a Fission Yeast TFIIIC Complex That Includes an Ortholog of the Human Regulatory Protein TFIIICbeta J. Biol. Chem., September 29, 2000; 275(40): 31480 - 31487. [Abstract] [Full Text] [PDF]