JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M003630200 on June 22, 2000

J. Biol. Chem., Vol. 275, Issue 37, 29162-29169, September 15, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/37/29162    most recent
M003630200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gomez, M.
Right arrow Articles by Perez, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gomez, M.
Right arrow Articles by Perez, F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Membrane Recruitment of Coatomer and Binding to Dilysine Signals Are Separate Events*

Marie GomezDagger , Suzie J. Scales§, Thomas E. Kreisdagger , and Franck Perez

From the Department of Cell Biology, University of Geneva, Sciences III, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland

It has previously been shown that transport of newly synthesized proteins and the structure of the Golgi complex are affected in the Chinese hamster ovary cell line ldlF, which bears a temperature-sensitive mutation in the Coat protein I (COPI) subunit epsilon -COP (Guo, Q., Vasile, E., and Krieger, M. (1994) J. Cell Biol. 125, 1213-1224; Hobbie, L., Fisher, A. S., Lee, S., Flint, A., and Krieger, M. (1994) J. Biol. Chem. 269, 20958-20970). Here, we pinpoint the site of the secretory block to an intermediate compartment between the endoplasmic reticulum (ER) and the Golgi complex and show that the distributions of ER-Golgi recycling proteins, such as KDEL receptor and p23, as well as resident Golgi proteins, such as mannosidase II, are accordingly affected. At the nonpermissive temperature, neither the stability of the COPI complex nor its recruitment to donor Golgi membranes is affected. However, the binding of coatomer to the dilysine-based ER-retrieval motif is impaired in the absence of epsilon -COP, suggesting that dilysine signal binding is not the major means of COPI recruitment. Because expression of the exogenous chimera of epsilon -COP and green fluorescent protein in ldlF cells at nonpermissive temperature rapidly restores the wild type properties, epsilon -COP is likely to play an important role in the cargo selection events mediated by COPI.

* This work was supported by grants from the Fonds National Suisse, the Canton de Genève, and the International Human Frontier Science Program (to T. E. K.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

This paper is dedicated to the memory of Thomas Kreis.

dagger Deceased.

Dagger Current address: Dept. of Molecular Neurobiology, Preclinical CNS Research, F. Hoffmann-La Roche Ltd., CH-4070 Basel, Switzerland.

§ Current address: Howard Hughes Medical Institute, Dept. of Molecular and Cellular Physiology, Beckman Center, Stanford University Medical School, Stanford, California 94305-5345.

To whom correspondence should be addressed: CNRS-UMR144, Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France. E-mail: franck.perez@curie.fr.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
V. Reiterer, S. Maier, H. H. Sitte, A. Kriz, M. A. Ruegg, H.-P. Hauri, M. Freissmuth, and H. Farhan
Sec24- and ARFGAP1-Dependent Trafficking of GABA Transporter-1 Is a Prerequisite for Correct Axonal Targeting
J. Neurosci., November 19, 2008; 28(47): 12453 - 12464.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Farhan, V. Reiterer, A. Kriz, H.-P. Hauri, M. Pavelka, H. H. Sitte, and M. Freissmuth
Signal-dependent export of GABA transporter 1 from the ER-Golgi intermediate compartment is specified by a C-terminal motif
J. Cell Sci., March 15, 2008; 121(6): 753 - 761.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. E. McBride, J. Li, and C. E. Machamer
The Cytoplasmic Tail of the Severe Acute Respiratory Syndrome Coronavirus Spike Protein Contains a Novel Endoplasmic Reticulum Retrieval Signal That Binds COPI and Promotes Interaction with Membrane Protein
J. Virol., March 1, 2007; 81(5): 2418 - 2428.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. D. Chandrasekhar, T. Bas, and W. R. Kobertz
KCNE1 Subunits Require Co-assembly with K+ Channels for Efficient Trafficking and Cell Surface Expression
J. Biol. Chem., December 29, 2006; 281(52): 40015 - 40023.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Oka, D. Ungar, F. M. Hughson, and M. Krieger
The COG and COPI Complexes Interact to Control the Abundance of GEARs, a Subset of Golgi Integral Membrane Proteins
Mol. Biol. Cell, May 1, 2004; 15(5): 2423 - 2435.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Llorente, S. U. Lauvrak, B. van Deurs, and K. Sandvig
Induction of Direct Endosome to Endoplasmic Reticulum Transport in Chinese Hamster Ovary (CHO) Cells (LdlF) with a Temperature-sensitive Defect in {epsilon}-Coatomer Protein ({epsilon}-COP)
J. Biol. Chem., September 12, 2003; 278(37): 35850 - 35855.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.