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J. Biol. Chem., Vol. 275, Issue 38, 29556-29561, September 22, 2000
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From the Division of Biological Sciences, Institute of Scientific
and Industrial Research, Osaka University, Core Research for
Evolutional Science and Technology (CREST) of the Japan Science and
Technology Corporation, Osaka 567-0047, Japan
Immunofluorescence analysis indicated that
VHA-11, the C subunit of Caenorhabditis elegans
V-ATPase, was localized in dot-like structures around the nuclei of
early embryonic cells and was also detected in embryonic intestinal
cells after comma stage. Vital staining with acridine orange
showed that the intestinal cells had acidic compartments generated by
V-ATPase, consistent with the intracellular localization of VHA-11. RNA
interference could efficiently silence vha-11 gene
expression: introduction of vha-11 double strand RNA led to
embryonic lethality. Worms injected with the vha-11 double
strand RNA produced embryos that became lethal. The development of
embryos was arrested at various stages. However, their numbers
gradually decreased, and the worms eventually became sterile due to the
failure of ovulation. Similar results were obtained for RNA
interference of the V-ATPase proteolipid genes. These results
suggest that V-ATPases, and thus inside-acidic organelles, are required for ovulation and embryogenesis.
Requirement of V-ATPase for Ovulation and Embryogenesis in
Caenorhabditis elegans*
*
This work was supported by a grant from the Ministry of
Education, Science and Culture of Japan.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Tel.: 81-6-6879-8480;
Fax: 81-6-6875-5724; E-mail: m-futai@sanken.osaka-u.ac.jp.
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