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J. Biol. Chem., Vol. 275, Issue 38, 29623-29627, September 22, 2000
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From the Loss of function of S-II makes yeast sensitive to
6-azauracil. Here, we identified a multi-copy suppressor gene of this
phenotype, termed SSM1 (suppressor of 6-azauracil
sensitivity of the S-II null mutant 1), that encodes a novel protein
consisting of 280 amino acid residues. Although both the
SSM1 null mutant and the S-II/SSM1 double null
mutant were viable under normal growth conditions, they resembled the
S-II null mutant in being sensitive to 6-azauracil. Expression of the SSM1 gene was found to be repressed in the
S-II null mutant but was restored by overexpression of
chimeric S-II molecules that were able to stimulate transcription
elongation by RNA polymerase II in vitro. Furthermore, we
identified two transcription arrest sites within the transcription unit
of the SSM1 gene in vitro that could be relieved by S-II.
These results indicate that S-II confers yeast resistance to
6-azauracil by stimulating transcription elongation of the SSM1 gene.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) D26043.
Transcription Elongation Factor S-II Confers Yeast Resistance to
6-Azauracil by Enhancing Expression of the SSM1 Gene*
§,§,, and
Graduate School of Pharmaceutical Sciences,
University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan and ¶ Natori
Special Laboratory, The Institute of Physical and Chemical Research,
Hirosawa 2-1, Wako-shi, Saitama 351-0198, Japan
*
This work was supported by a grant-in-aid from the Ministry
of Education, Science, Sports and Culture of Japan and by Core Research
for Evolutional Science and Technology of the Japan Science and
Technology Cooperation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
81-48-467-9437; Fax: 81-48-462-4693; E-mail:
natori@postman.riken.go.ap.
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