Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M004393200 on July 10, 2000

J. Biol. Chem., Vol. 275, Issue 38, 29654-29659, September 22, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/38/29654    most recent
M004393200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Küssel-Andermann, P.
Right arrow Articles by Petit, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Küssel-Andermann, P.
Right arrow Articles by Petit, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Unconventional Myosin VIIA Is a Novel A-kinase-anchoring Protein*

Polonca Küssel-AndermannDagger , Aziz El-AmraouiDagger , Saaid SafieddineDagger , Jean-Pierre HardelinDagger , Sylvie NouailleDagger , Jacques Camonis§, and Christine PetitDagger

From the Dagger  Unité de Génétique des Déficits Sensoriels, CNRS URA 1968, 25 rue du Dr. Roux, Institut Pasteur, 75724 Paris cedex 15, France and § INSERM U248, Institut Curie, 75248 Paris cedex 05, France

To gain an insight into the cellular function of the unconventional myosin VIIA, we sought proteins interacting with its tail region, using the yeast two-hybrid system. Here we report on one of the five candidate interactors we identified, namely the type Ialpha regulatory subunit (RIalpha ) of protein kinase A. The interaction of RIalpha with myosin VIIA tail was demonstrated by coimmunoprecipitation from transfected HEK293 cells. Analysis of deleted constructs in the yeast two-hybrid system showed that the interaction of myosin VIIA with RIalpha involves the dimerization domain of RIalpha . In vitro binding assays identified the C-terminal "4.1, ezrin, radixin, moesin" (FERM)-like domain of myosin VIIA as the interacting domain. In humans and mice, mutations in the myosin VIIA gene underlie hereditary hearing loss, which may or may not be associated with visual deficiency. Immunohistofluorescence revealed that myosin VIIA and RIalpha are coexpressed in the outer hair cells of the cochlea and rod photoreceptor cells of the retina. Our results strongly suggest that myosin VIIA is a novel protein kinase A-anchoring protein that targets protein kinase A to definite subcellular sites of these sensory cells.

* This work was supported by European Community Grant QLG2-CT-1999-00988; grants from Retina-France, A. and M. Suchert, and Forschung contra Blindheit-Initiative Usher Syndrom; and a donation from C. and J-P. Bernais.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 33 1 45 68 88 50 or 33 1 45 68 88 90; Fax: 33 1 45 67 69 78; E-mail: cpetit@pasteur.fr.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
O. M. Faruque, D. Le-Nguyen, A.-D. Lajoix, E. Vives, P. Petit, D. Bataille, and E.-H. Hani
Cell-permeable peptide-based disruption of endogenous PKA-AKAP complexes: a tool for studying the molecular roles of AKAP-mediated PKA subcellular anchoring
Am J Physiol Cell Physiol, February 1, 2009; 296(2): C306 - C316.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Jarnaess, A. Ruppelt, A. J. Stokka, B. Lygren, J. D. Scott, and K. Tasken
Dual Specificity A-kinase Anchoring Proteins (AKAPs) Contain an Additional Binding Region That Enhances Targeting of Protein Kinase A Type I
J. Biol. Chem., November 28, 2008; 283(48): 33708 - 33718.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. Legendre, S. Safieddine, P. Kussel-Andermann, C. Petit, and A. El-Amraoui
{alpha}II-{beta}V spectrin bridges the plasma membrane and cortical lattice in the lateral wall of the auditory outer hair cells
J. Cell Sci., October 15, 2008; 121(20): 3347 - 3356.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
N. Michalski, V. Michel, A. Bahloul, G. Lefevre, J. Barral, H. Yagi, S. Chardenoux, D. Weil, P. Martin, J.-P. Hardelin, et al.
Molecular Characterization of the Ankle-Link Complex in Cochlear Hair Cells and Its Role in the Hair Bundle Functioning
J. Neurosci., June 13, 2007; 27(24): 6478 - 6488.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
H. Kremer, E. van Wijk, T. Marker, U. Wolfrum, and R. Roepman
Usher syndrome: molecular links of pathogenesis, proteins and pathways
Hum. Mol. Genet., October 15, 2006; 15(suppl_2): R262 - R270.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. I. Tuxworth, S. Stephens, Z. C. Ryan, and M. A. Titus
Identification of a Myosin VII-Talin Complex
J. Biol. Chem., July 15, 2005; 280(28): 26557 - 26564.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
I. Bossis and C. A. Stratakis
Minireview: PRKAR1A: Normal and Abnormal Functions
Endocrinology, December 1, 2004; 145(12): 5452 - 5458.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
D. D. Mruk and C. Y. Cheng
Sertoli-Sertoli and Sertoli-Germ Cell Interactions and Their Significance in Germ Cell Movement in the Seminiferous Epithelium during Spermatogenesis
Endocr. Rev., October 1, 2004; 25(5): 747 - 806.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Veugelers, D. Wilkes, K. Burton, D. A. McDermott, Y. Song, M. M. Goldstein, K. La Perle, C. J. Vaughan, A. O'Hagan, K. R. Bennett, et al.
Comparative PRKAR1A genotype-phenotype analyses in humans with Carney complex and prkar1a haploinsufficient mice
PNAS, September 28, 2004; 101(39): 14222 - 14227.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
K. TASKEN and E. M. AANDAHL
Localized Effects of cAMP Mediated by Distinct Routes of Protein Kinase A
Physiol Rev, January 1, 2004; 84(1): 137 - 167.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
G. K. Carnegie and J. D. Scott
A-kinase anchoring proteins and neuronal signaling mechanisms
Genes & Dev., July 1, 2003; 17(13): 1557 - 1568.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. M. Alto, S. H. Soderling, N. Hoshi, L. K. Langeberg, R. Fayos, P. A. Jennings, and J. D. Scott
Bioinformatic design of A-kinase anchoring protein-in silico: A potent and selective peptide antagonist of type II protein kinase A anchoring
PNAS, April 15, 2003; 100(8): 4445 - 4450.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Inoue and M. Ikebe
Characterization of the Motor Activity of Mammalian Myosin VIIA
J. Biol. Chem., February 7, 2003; 278(7): 5478 - 5487.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. B. Shah and R. L. Lieber
Simultaneous Imaging and Functional Assessment of Cytoskeletal Protein Connections in Passively Loaded Single Muscle Cells
J. Histochem. Cytochem., January 1, 2003; 51(1): 19 - 29.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. L. Cyr, R. A. Dumont, and P. G. Gillespie
Myosin-1c Interacts with Hair-Cell Receptors through Its Calmodulin-Binding IQ Domains
J. Neurosci., April 1, 2002; 22(7): 2487 - 2495.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A.-S. Lebre, L. Jamot, J. Takahashi, N. Spassky, C. Leprince, N. Ravise, C. Zander, H. Fujigasaki, P. Kussel-Andermann, C. Duyckaerts, et al.
Ataxin-7 interacts with a Cbl-associated protein that it recruits into neuronal intranuclear inclusions
Hum. Mol. Genet., May 1, 2001; 10(11): 1201 - 1213.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Barradeau, T. Imaizumi-Scherrer, M. C. Weiss, and D. M. Faust
Muscle-regulated expression and determinants for neuromuscular junctional localization of the mouse RIalpha regulatory subunit of cAMP- dependent protein kinase
PNAS, April 5, 2001; (2001) 81393598.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
D Diviani and J. Scott
AKAP signaling complexes at the cytoskeleton
J. Cell Sci., January 4, 2001; 114(8): 1431 - 1437.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Barradeau, T. Imaizumi-Scherrer, M. C. Weiss, and D. M. Faust
Muscle-regulated expression and determinants for neuromuscular junctional localization of the mouse RIalpha regulatory subunit of cAMP- dependent protein kinase
PNAS, April 24, 2001; 98(9): 5037 - 5042.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement