JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M000571200 on July 14, 2000

J. Biol. Chem., Vol. 275, Issue 38, 29694-29700, September 22, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/38/29694    most recent
M000571200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schweizer, A.
Right arrow Articles by Rohrer, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schweizer, A.
Right arrow Articles by Rohrer, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

A Di-aromatic Motif in the Cytosolic Tail of the Mannose Receptor Mediates Endosomal Sorting*

Anja SchweizerDagger , Philip D. Stahl§, and Jack RohrerDagger ||

From the Dagger  Friedrich Miescher Institut, Maulbeerstrasse 66, 4058 Basel, Switzerland and the § Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110

The mannose receptor (MR), the prototype of a new family of multilectin receptor proteins important in innate immunity, undergoes rapid internalization and recycling from the endosomal system back to the cell surface. Sorting of the MR in endosomes prevents the receptor from entering lysosomes where it would be degraded. Here, we focused on a diaromatic sequence (Tyr18-Phe19) in the MR cytoplasmic tail as an endosomal sorting signal. The subcellular distribution of chimeric constructs between the MR and the cation-dependent mannose 6-phosphate receptor was assessed by Percoll density gradients and cell surface assays. Unlike the wild type constructs, mutant receptors with alanine substitutions of Tyr18-Phe19 were highly missorted to lysosomes, indicating that the di-aromatic motif of the MR cytoplasmic tail mediates sorting in endosomes. Within this sequence Tyr18 is the key residue with Phe19 contributing to this function. Moreover, Tyr18 was also found to be essential for internalization, consistent with the presence of overlapping signals for internalization and endosomal sorting in the cytosolic tail of the MR.

A di-aromatic amino acid sequence in the cytosolic tail has now been shown to function in two receptors known to be internalized from the plasma membrane, the MR and the cation-dependent mannose 6-phosphate receptor. This feature therefore appears to be a general determinant for endosomal sorting.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Supported by National Institutes of Health Grant 2R01AI20015-16.

|| Supported by a Prof. Max Cloëtta fellowship. To whom correspondence should be addressed. Tel.: 41 61 697 7609; Fax: 41 61 697 3976; E-mail: Rohrer@fmi.ch.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
A. K. Azad, J. B. Torrelles, and L. S. Schlesinger
Mutation in the DC-SIGN cytoplasmic triacidic cluster motif markedly attenuates receptor activity for phagocytosis and endocytosis of mannose-containing ligands by human myeloid cells
J. Leukoc. Biol., December 1, 2008; 84(6): 1594 - 1603.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. M. Dan, R. M. Kelly, C. K. Lee, and S. M. Levitz
Role of the Mannose Receptor in a Murine Model of Cryptococcus neoformans Infection
Infect. Immun., June 1, 2008; 76(6): 2362 - 2367.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. Mercanti, C. Blanc, Y. Lefkir, P. Cosson, and F. Letourneur
Acidic clusters target transmembrane proteins to the contractile vacuole in Dictyostelium cells
J. Cell Sci., March 1, 2006; 119(5): 837 - 845.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. K. Mansour, E. Latz, and S. M. Levitz
Cryptococcus neoformans Glycoantigens Are Captured by Multiple Lectin Receptors and Presented by Dendritic Cells.
J. Immunol., March 1, 2006; 176(5): 3053 - 3061.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
P. B. Kang, A. K. Azad, J. B. Torrelles, T. M. Kaufman, A. Beharka, E. Tibesar, L. E. DesJardin, and L. S. Schlesinger
The human macrophage mannose receptor directs Mycobacterium tuberculosis lipoarabinomannan-mediated phagosome biogenesis
J. Exp. Med., October 3, 2005; 202(7): 987 - 999.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. C. Berzat, J. E. Buss, E. J. Chenette, C. A. Weinbaum, A. Shutes, C. J. Der, A. Minden, and A. D. Cox
Transforming Activity of the Rho Family GTPase, Wrch-1, a Wnt-regulated Cdc42 Homolog, Is Dependent on a Novel Carboxyl-terminal Palmitoylation Motif
J. Biol. Chem., September 23, 2005; 280(38): 33055 - 33065.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
V. Le Cabec, L. J. Emorine, I. Toesca, C. Cougoule, and I. Maridonneau-Parini
The human macrophage mannose receptor is not a professional phagocytic receptor
J. Leukoc. Biol., June 1, 2005; 77(6): 934 - 943.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
D. J. Vigerust, B. S. Egan, and V. L. Shepherd
HIV-1 Nef mediates post-translational down-regulation and redistribution of the mannose receptor
J. Leukoc. Biol., April 1, 2005; 77(4): 522 - 534.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Stockli and J. Rohrer
The Palmitoyltransferase of the Cation-dependent Mannose 6-Phosphate Receptor Cycles between the Plasma Membrane and Endosomes
Mol. Biol. Cell, June 1, 2004; 15(6): 2617 - 2626.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Kato, S. Khan, N. Gonzalez, B. P. O'Neill, K. J. McDonald, B. J. Cooper, N. Z. Angel, and D. N. J. Hart
Hodgkin's Lymphoma Cell Lines Express a Fusion Protein Encoded by Intergenically Spliced mRNA for the Multilectin Receptor DEC-205 (CD205) and a Novel C-type Lectin Receptor DCL-1
J. Biol. Chem., September 5, 2003; 278(36): 34035 - 34041.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
I. Porcaro, M. Vidal, S. Jouvert, P. D. Stahl, and J. Giaimis
Mannose receptor contribution to Candida albicans phagocytosis by murine E-clone J774 macrophages
J. Leukoc. Biol., August 1, 2003; 74(2): 206 - 215.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. East, S. Rushton, M. E. Taylor, and C. M. Isacke
Characterization of Sugar Binding by the Mannose Receptor Family Member, Endo180
J. Biol. Chem., December 20, 2002; 277(52): 50469 - 50475.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Howard and C. M. Isacke
The C-type Lectin Receptor Endo180 Displays Internalization and Recycling Properties Distinct from Other Members of the Mannose Receptor Family
J. Biol. Chem., August 23, 2002; 277(35): 32320 - 32331.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.