|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 38, 29754-29760, September 22, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Biomedical Sciences, Tufts University School
of Veterinary Medicine, North Grafton, Massachusetts 01536
Cell swelling stimulates phosphoinositide
3-kinase (PI3K) and mitogen-activated protein kinase (MAPK) in
hepatocytes, and the PI3K signaling pathway is involved in
cAMP-mediated translocation of sinusoidal
Na+/taurocholate (TC) cotransporter (Ntcp) to the
plasma membrane. We determined whether cell swelling also stimulates TC
uptake and Ntcp translocation via the PI3K and/or MAPK signaling
pathway. All studies were conducted in isolated rat hepatocytes.
Hepatocyte swelling induced by hypotonic media resulted in: 1)
time- and medium osmolarity-dependent increases in TC
uptake, 2) an increase in the Vmax of
Na+/TC cotransport, and 3) wortmannin-sensitive increases
in TC uptake and plasma membrane Ntcp mass. Hepatocyte swelling also
induced wortmannin-sensitive activation of PI3K, protein kinase
B, and p70S6K. Rapamycin, an inhibitor of
p70S6K, inhibited cell swelling-induced activation of
p70S6K but failed to inhibit cell swelling-induced
stimulation of TC uptake. Because PD98095, an inhibitor of MAPK, did
not inhibit cell swelling-induced increases in TC uptake, it is
unlikely that the effect of cell swelling on TC uptake is mediated via
the MAPK signaling pathway. Taken together, these results indicate that 1) cell swelling stimulates TC uptake by translocating Ntcp to the
plasma membrane, 2) this effect is mediated via the PI3K, but not MAPK,
signaling pathway, and 3) protein kinase B, but not p70S6K,
is a likely downstream effector of PI3K.
Cell Swelling-induced Translocation of Rat Liver
Na+/Taurocholate Cotransport Polypeptide Is Mediated via
the Phosphoinositide 3-Kinase Signaling Pathway*
*
This work was supported in part by Grant DK-33436 from the
National Institutes of Health.The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Biomedical
Sciences, Tufts University School of Veterinary Medicine, 200 Westboro
Rd., North Grafton, MA 01536. Tel.: 508-839-8788; Fax: 508-839-8787;
E-mail: SANWER@INFONET.TUFTS.EDU.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. T. Barfod, A. L. Moore, M. W. Roe, and S. D. Lidofsky Ca2+-activated IK1 Channels Associate with Lipid Rafts upon Cell Swelling and Mediate Volume Recovery J. Biol. Chem., March 23, 2007; 282(12): 8984 - 8993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-Z. Lan, P. Y. T. Wang, and C. E. Hill Modulation of hepatocellular swelling-activated K+ currents by phosphoinositide pathway-dependent protein kinase C Am J Physiol Cell Physiol, July 1, 2006; 291(1): C93 - C103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Sabnis, D. Jelovac, B. Long, and A. Brodie The Role of Growth Factor Receptor Pathways in Human Breast Cancer Cells Adapted to Long-term Estrogen Deprivation Cancer Res., May 1, 2005; 65(9): 3903 - 3910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. McConkey, H. Gillin, C. R. L. Webster, and M. S. Anwer Cross-talk between Protein Kinases C{zeta} and B in Cyclic AMP-mediated Sodium Taurocholate Co-transporting Polypeptide Translocation in Hepatocytes J. Biol. Chem., May 14, 2004; 279(20): 20882 - 20888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Schliess, R. Reissmann, R. Reinehr, S. vom Dahl, and D. Haussinger Involvement of Integrins and Src in Insulin Signaling toward Autophagic Proteolysis in Rat Liver J. Biol. Chem., May 14, 2004; 279(20): 21294 - 21301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. v. Dahl, F. Schliess, R. Reissmann, B. Gorg, O. Weiergraber, M. Kocalkova, F. Dombrowski, and D. Haussinger Involvement of Integrins in Osmosensing and Signaling toward Autophagic Proteolysis in Rat Liver J. Biol. Chem., July 11, 2003; 278(29): 27088 - 27095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Beuers, G. U. Denk, C. J. Soroka, R. Wimmer, C. Rust, G. Paumgartner, and J. L. Boyer Taurolithocholic Acid Exerts Cholestatic Effects via Phosphatidylinositol 3-Kinase-dependent Mechanisms in Perfused Rat Livers and Rat Hepatocyte Couplets J. Biol. Chem., May 9, 2003; 278(20): 17810 - 17818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. L. Webster, U. Srinivasulu, M. Ananthanarayanan, F. J. Suchy, and M. S. Anwer Protein Kinase B/Akt Mediates cAMP- and Cell Swelling-stimulated Na+/Taurocholate Cotransport and Ntcp Translocation J. Biol. Chem., August 2, 2002; 277(32): 28578 - 28583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. L. Webster, C. Blanch, and M. S. Anwer Role of PP2B in cAMP-induced dephosphorylation and translocation of NTCP Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G44 - G50. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Shi, S. Barnes, M. Coca-Prados, and M. E. M. Kelly Protein Tyrosine Kinase and Protein Phosphatase Signaling Pathways Regulate Volume-Sensitive Chloride Currents in a Nonpigmented Ciliary Epithelial Cell Line Invest. Ophthalmol. Vis. Sci., May 1, 2002; 43(5): 1525 - 1532. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |