|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 39, 30220-30225, September 29, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Activation of protein kinase C (PKC) can result
from stimulation of the receptor-G protein-phospholipase C (PLC
Molecular Mechanism of the Inhibition of Phospholipase C
3 by Protein Kinase C*
,,
Department of Biochemistry and Molecular
Biology, University of Texas Medical School, Houston, Texas 77225, the § Department of Medical Biochemistry and Genetics,
Center for Cancer Biology and Nutrition, Institute of Biosciences and
Technology, Texas A&M University Health Science Center,
Houston, Texas 77030, and the ¶ Department of Biology,
California Institute of Technology, Pasadena, California 91125
)
pathway. In turn, phosphorylation of PLC by PKC may play a role in
the regulation of receptor-mediated phosphatidylinositide (PI) turnover and intracellular Ca2+ release. Activation of
endogenous PKC by phorbol 12-myristate 13-acetate inhibited both
G
q-coupled (oxytocin and M1 muscarinic) and
Gi-coupled (formyl-Met-Leu-Phe) receptor-stimulated PI
turnover by 50-100% in PHM1, HeLa, COSM6, and RBL-2H3 cells
expressing PLC3. Activation of conventional PKCs with
thymeleatoxin similarly inhibited oxytocin or formyl-Met-Leu-Phe
receptor-stimulated PI turnover. The PKC inhibitory effect was also
observed when PLC3 was stimulated directly by
Gq or G
in overexpression assays. PKC
phosphorylated PLC3 at the same predominant site
in vivo and in vitro. Peptide sequencing of
in vitro phosphorylated recombinant PLC3 and
site-directed mutagenesis identified Ser1105 as the
predominant phosphorylation site. Ser1105 is also
phosphorylated by protein kinase A (PKA; Yue, C., Dodge, K. L.,
Weber, G., and Sanborn, B. M. (1998) J. Biol.
Chem. 273, 18023-18027). Similar to PKA, the inhibition by PKC
of Gq-stimulated PLC3 activity was
completely abolished by mutation of Ser1105 to Ala. In
contrast, mutation of Ser1105 or
Ser26, another putative phosphorylation target, to Ala had
no effect on inhibition of G-stimulated PLC3
activity by PKC or PKA. These data indicate that PKC and PKA act
similarly in that they inhibit Gq-stimulated
PLC3 as a result of phosphorylation of Ser1105. Moreover, PKC and PKA both inhibit
G-stimulated activity by mechanisms that do not involve
Ser1105.
*
This work was supported in part by National
Institutes of Health Grant HD09618 (to B. M. S.).The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Biochemistry and Molecular Biology, University of Texas Medical School, P. O. Box 20708, Houston, TX 77225. Tel.: 713-500-6064; Fax:
713-500-0652; E-mail: Barbara.M.Sanborn@uth.tmc.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. I. A. Roach, R. A. Rebres, I. D. C. Fraser, D. L. DeCamp, K.-M. Lin, P. C. Sternweis, M. I. Simon, and W. E. Seaman Signaling and Cross-talk by C5a and UDP in Macrophages Selectively Use PLC{beta}3 to Regulate Intracellular Free Calcium J. Biol. Chem., June 20, 2008; 283(25): 17351 - 17361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zhong, D. A. Murtazina, J. Phillips, C.-Y. Ku, and B. M. Sanborn Multiple Signals Regulate Phospholipase CBeta3 in Human Myometrial Cells Biol Reprod, June 1, 2008; 78(6): 1007 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ito, T. Shikano, S. Oda, T. Horiguchi, S. Tanimoto, T. Awaji, H. Mitani, and S. Miyazaki Difference in Ca2+ Oscillation-Inducing Activity and Nuclear Translocation Ability of PLCZ1, an Egg-Activating Sperm Factor Candidate, Between Mouse, Rat, Human, and Medaka Fish Biol Reprod, June 1, 2008; 78(6): 1081 - 1090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Violin, S. M. DeWire, W. G. Barnes, and R. J. Lefkowitz G Protein-coupled Receptor Kinase and beta-Arrestin-mediated Desensitization of the Angiotensin II Type 1A Receptor Elucidated by Diacylglycerol Dynamics J. Biol. Chem., November 24, 2006; 281(47): 36411 - 36419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ball, J. W. Wang, S. Wong, B. Zielnik, J. Mitchell, N. Wang, M. B. Stemerman, and B. F. Mitchell Phorbol ester treatment of human myometrial cells suppresses expression of oxytocin receptor through a mechanism that does not involve activator protein-1 Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E922 - E928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Frecker, S. Munk, H. Wang, and C. Whiteside Mesangial cell-reduced Ca2+ signaling in high glucose is due to inactivation of phospholipase C-{beta}3 by protein kinase C Am J Physiol Renal Physiol, November 1, 2005; 289(5): F1078 - F1087. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. Sanborn, C.-Y. Ku, S. Shlykov, and L. Babich Molecular Signaling Through G-Protein-Coupled Receptors and the Control of Intracellular Calcium in Myometrium Reproductive Sciences, October 1, 2005; 12(7): 479 - 487. [Abstract] [PDF]
|
|
|
|
 |
|
 M. H. Zhu, M. Chae, H. J. Kim, Y. M. Lee, M. J. Kim, N. G. Jin, D. K. Yang, I. So, and K. W. Kim Desensitization of canonical transient receptor potential channel 5 by protein kinase C Am J Physiol Cell Physiol, September 1, 2005; 289(3): C591 - C600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hisatsune, K. Nakamura, Y. Kuroda, T. Nakamura, and K. Mikoshiba Amplification of Ca2+ Signaling by Diacylglycerol-mediated Inositol 1,4,5-Trisphosphate Production J. Biol. Chem., March 25, 2005; 280(12): 11723 - 11730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Morgan, A. Skippen, B. Segui, A. Ball, V. Allen-Baume, B. Larijani, J. Murray-Rust, N. McDonald, G. Sapkota, N. Morrice, et al. Phosphorylation of a Distinct Structural Form of Phosphatidylinositol Transfer Protein {alpha} at Ser166 by Protein Kinase C Disrupts Receptor-mediated Phospholipase C Signaling by Inhibiting Delivery of Phosphatidylinositol to Membranes J. Biol. Chem., November 5, 2004; 279(45): 47159 - 47171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Schaefer, H. Mischak, S. Schnell, A. Griese, R. Iakubov, G. Riepenhausen, and C. Schofl Mechanisms of Arginine-Vasopressin-Induced Ca2+ Oscillations in {beta}-Cells (HIT-T15): A Role for Oscillating Protein Kinase C Endocrinology, October 1, 2004; 145(10): 4635 - 4644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Kerrigan, M. Gaur, R. P. Murphy, S. J. Shattil, and A. D. Leavitt Caspase-12: a developmental link between G-protein-coupled receptors and integrin {alpha}IIb{beta}3 activation Blood, September 1, 2004; 104(5): 1327 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chen, E. J. Dell, F. Lin, J. Sai, and H. E. Hamm RACK1 Regulates Specific Functions of G{beta}{gamma} J. Biol. Chem., April 23, 2004; 279(17): 17861 - 17868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chakrabarti, N.-J. Liu, and A. R. Gintzler Reciprocal modulation of phospholipase C{beta} isoforms: Adaptation to chronic morphine PNAS, November 11, 2003; 100(23): 13686 - 13691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Venkatachalam, F. Zheng, and D. L. Gill Regulation of Canonical Transient Receptor Potential (TRPC) Channel Function by Diacylglycerol and Protein Kinase C J. Biol. Chem., August 1, 2003; 278(31): 29031 - 29040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Babwah, L. B. Dale, and S. S. G. Ferguson Protein Kinase C Isoform-specific Differences in the Spatial-Temporal Regulation and Decoding of Metabotropic Glutamate Receptor1a-stimulated Second Messenger Responses J. Biol. Chem., February 7, 2003; 278(7): 5419 - 5426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Dell, J. Connor, S. Chen, E. G. Stebbins, N. P. Skiba, D. Mochly-Rosen, and H. E. Hamm The beta gamma Subunit of Heterotrimeric G Proteins Interacts with RACK1 and Two Other WD Repeat Proteins J. Biol. Chem., December 13, 2002; 277(51): 49888 - 49895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Young, S. V. Wu, and E. Rozengurt Ca2+-stimulated Ca2+ Oscillations Produced by the Ca2+-sensing Receptor Require Negative Feedback by Protein Kinase C J. Biol. Chem., November 27, 2002; 277(49): 46871 - 46876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Castro, F. Dicker, J.-P. Vilardaga, C. Krasel, M. Bernhardt, and M. J. Lohse Dual Regulation of the Parathyroid Hormone (PTH)/PTH-Related Peptide Receptor Signaling by Protein Kinase C and {beta}-Arrestins Endocrinology, October 1, 2002; 143(10): 3854 - 3865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Nash, M. J. Schell, P. J. Atkinson, N. R. Johnston, S. R. Nahorski, and R. A. J. Challiss Determinants of Metabotropic Glutamate Receptor-5-mediated Ca2+ and Inositol 1,4,5-Trisphosphate Oscillation Frequency. RECEPTOR DENSITY VERSUS AGONIST CONCENTRATION J. Biol. Chem., September 20, 2002; 277(39): 35947 - 35960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Barhoumi, Y. Mouneimne, I. Awooda, S. H. Safe, K. C. Donnelly, and R. C. Burghardt Characterization of Calcium Oscillations in Normal and Benzo[a]pyrene-Treated Clone 9 Cells Toxicol. Sci., August 1, 2002; 68(2): 444 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Nasuhoglu, S. Feng, Y. Mao, I. Shammat, M. Yamamato, S. Earnest, M. Lemmon, and D. W. Hilgemann Modulation of cardiac PIP2 by cardioactive hormones and other physiologically relevant interventions Am J Physiol Cell Physiol, July 1, 2002; 283(1): C223 - C234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Xu, P.-G. Suh, N. Marmy-Conus, R. B. Pearson, O. Y. Seok, L. Cocco, and R. S. Gilmour Phosphorylation of Nuclear Phospholipase C {beta}1 by Extracellular Signal-Regulated Kinase Mediates the Mitogenic Action of Insulin-Like Growth Factor I Mol. Cell. Biol., May 1, 2001; 21(9): 2981 - 2990. [Abstract] [Full Text]
|
|
|
|
|
|
A. Xu, Y. Wang, L. Y. Xu, and R. S. Gilmour Protein Kinase C alpha -mediated Negative Feedback Regulation Is Responsible for the Termination of Insulin-like Growth Factor I-induced Activation of Nuclear Phospholipase C beta 1 in Swiss 3T3 Cells J. Biol. Chem., April 27, 2001; 276(18): 14980 - 14986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Arthur, S. J. Matkovich, C. J. Mitchell, T. J. Biden, and E. A. Woodcock Evidence for Selective Coupling of alpha 1-Adrenergic Receptors to Phospholipase C-beta 1 in Rat Neonatal Cardiomyocytes J. Biol. Chem., September 28, 2001; 276(40): 37341 - 37346. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |