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J Biol Chem, Vol. 275, Issue 4, 2259-2264, January 28, 2000

The trans-Spliceosomal U4 RNA from the Monogenetic Trypanosomatid Leptomonas collosoma
CLONING AND IDENTIFICATION OF A TRANSCRIBED tRNA-LIKE ELEMENT THAT CONTROLS ITS EXPRESSION*

Liu LiDagger , Leo R. Otake§, Yu-xin Xu, and Shulamit MichaeliDagger

From the Dagger  Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel the § Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06536-0812, and the  Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel

U4 small nuclear RNA is essential for trans-splicing. Here we report the cloning of U4 snRNA gene from Leptomonas collosoma and analysis of elements controlling its expression. The trypanosome U4 RNA is the smallest known, it carries an Sm-like site, and has the potential for extensive intermolecular base pairing with the U6 RNA. Sequence analysis of the U4 locus indicates the presence of a tRNA-like element 86 base pairs upstream of the gene that is divergently transcribed to yield a stable small tRNA-like RNA. Two additional tRNA genes, tRNAPro and tRNAGly, were found upstream of this element. By stable expression of a tagged U4 RNA, we demonstrate that the tRNA-like gene, but not the upstream tRNA genes, is essential for U4 expression and that the B box but not the A Box of the tRNA-like gene is crucial for expression in vivo. Mapping the 2'-O-methyl groups on U4 and U6 small nuclear RNAs suggests the presence of modifications in canonical positions. However, the number of modified nucleotides is fewer than in mammalian homologues. The U4 genomic organization including both tRNA-like and tRNA genes may represent a relic whereby trypanosomatids "hired" tRNA genes to provide extragenic promoter elements. The close proximity of tRNA genes to the tRNA-like molecule in the U4 locus further suggests that the tRNA-like gene may have evolved from a tRNA member of this cluster.

* This work was supported by German-Israeli Foundation Grant I-353-078.03/94 and by National Institutes of Health Grant GM26154 (to Joan A. Steitz).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF204671.

To correspondence should be addressed. Tel.: 972-3-531-8068; Fax: 972-3-535-1824; E-mail: michaes@biu.mail.ac.il.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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