HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sidobre, S. Articles by Rivière, M. Search for Related Content PubMed PubMed Citation Articles by Sidobre, S. Articles by Rivière, M. Social Bookmarking                      What's this?

J Biol Chem, Vol. 275, Issue 4, 2415-2422, January 28, 2000

Lipoglycans Are Putative Ligands for the Human Pulmonary Surfactant Protein A Attachment to Mycobacteria
CRITICAL ROLE OF THE LIPIDS FOR LECTIN-CARBOHYDRATE RECOGNITION^*

Stéphane Sidobre, Jérome Nigou, Germain Puzo, and Michel Rivière

From the Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, 31077 Toulouse Cedex, France

The human pulmonary surfactant protein A (hSP-A) has been implicated in the early capture and phagocytosis of the pathogenic Mycobacterium tuberculosis by alveolar macrophages. In this report, we examined the interaction of alveolar proteinosis patient hSP-A with Mycobacterium bovis BCG, the vaccinating strain, as a model of pathogenic mycobacteria, and Mycobacterium smegmatis, a nonpathogenic strain. We found that hSP-A binds to the surface of M. bovis BCG, but also to a slightly lesser extent, to M. smegmatis, indicating that hSP-A does not discriminate between virulent and nonpathogenic strains. Among the various glycoconjugates isolated from the mycobacterial envelope, we found that the best ligands are the two major lipoglycans: the mannosylated lipoarabinomannan (ManLAM) and the lipomannan. In contrast, the mannose-capped arabinomannan, structurally close to the ManLAM, as well as the LAMs from the non pathogenic M. smegmatis are poorly recognized by hSP-A. These results clearly show that the presence of both the terminal mannose residues and the phophatidyl-myo-inositol anchor are necessary to achieve the highest binding affinity. Selective removal of either the terminal mannose or the acyl residues esterifying the glycerol moiety of the ManLAM abrogates the interaction with hSP-A, further supporting the notion that the hSP-A recognition of the carbohydrate epitopes of the lipoglycans is dependent of the presence of the fatty acids.

---

^* This work was supported by grants from the Mission Scientifique et Technique du Ministère de l'Education Nationale, de l'Enseignement Supérieur et de la Recherche (ACC SV6 9506005 and ACC SV14 1411587) and from the Région Midi-Pyrénées (RECH/9702343).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: IPBS CNRS, 205 route de Narbonne, 31077 Toulouse cedex, France. Tel.: 33-5-61-17-55-58; Fax: 33-5-61-17-59-94; E-mail: riviere@ipbs.fr.

---

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				H. Sahly, Y. Keisari, E. Crouch, N. Sharon, and I. Ofek Recognition of Bacterial Surface Polysaccharides by Lectins of the Innate Immune System and Its Contribution to Defense against Infection: the Case of Pulmonary Pathogens Infect. Immun., April 1, 2008; 76(4): 1322 - 1332. [Full Text] [PDF]

				R. Deb, F. Shakib, K. Reid, and H. Clark Major House Dust Mite Allergens Dermatophagoides pteronyssinus 1 and Dermatophagoides farinae 1 Degrade and Inactivate Lung Surfactant Proteins A and D J. Biol. Chem., December 21, 2007; 282(51): 36808 - 36819. [Abstract] [Full Text] [PDF]

				E. Doz, S. Rose, J. Nigou, M. Gilleron, G. Puzo, F. Erard, B. Ryffel, and V. F. J. Quesniaux Acylation Determines the Toll-like receptor (TLR)-dependent Positive Versus TLR2-, Mannose Receptor-, and SIGNR1-independent Negative Regulation of Pro-inflammatory Cytokines by Mycobacterial Lipomannan J. Biol. Chem., September 7, 2007; 282(36): 26014 - 26025. [Abstract] [Full Text] [PDF]

				A. Ragas, L. Roussel, G. Puzo, and M. Riviere The Mycobacterium tuberculosis Cell-surface Glycoprotein Apa as a Potential Adhesin to Colonize Target Cells via the Innate Immune System Pulmonary C-type Lectin Surfactant Protein A J. Biol. Chem., February 23, 2007; 282(8): 5133 - 5142. [Abstract] [Full Text] [PDF]

				L. Hall-Stoodley, G. Watts, J. E. Crowther, A. Balagopal, J. B. Torrelles, J. Robison-Cox, R. F. Bargatze, A. G. Harmsen, E. C. Crouch, and L. S. Schlesinger Mycobacterium tuberculosis Binding to Human Surfactant Proteins A and D, Fibronectin, and Small Airway Epithelial Cells under Shear Conditions. Infect. Immun., June 1, 2006; 74(6): 3587 - 3596. [Abstract] [Full Text] [PDF]

				K. J. C. Gibson, M. Gilleron, P. Constant, B. Sichi, G. Puzo, G. S. Besra, and J. Nigou A Lipomannan Variant with Strong TLR-2-dependent Pro-inflammatory Activity in Saccharothrix aerocolonigenes J. Biol. Chem., August 5, 2005; 280(31): 28347 - 28356. [Abstract] [Full Text] [PDF]

				J. B. Torrelles, K.-H. Khoo, P. A. Sieling, R. L. Modlin, N. Zhang, A. M. Marques, A. Treumann, C. D. Rithner, P. J. Brennan, and D. Chatterjee Truncated Structural Variants of Lipoarabinomannan in Mycobacterium leprae and an Ethambutol-resistant Strain of Mycobacterium tuberculosis J. Biol. Chem., September 24, 2004; 279(39): 41227 - 41239. [Abstract] [Full Text] [PDF]

				K. Kudo, H. Sano#, H. Takahashi, K. Kuronuma, S.-i. Yokota, N. Fujii, K.-i. Shimada, I. Yano, Y. Kumazawa, D. R. Voelker, et al. Pulmonary Collectins Enhance Phagocytosis of Mycobacterium avium through Increased Activity of Mannose Receptor J. Immunol., June 15, 2004; 172(12): 7592 - 7602. [Abstract] [Full Text] [PDF]

				K. J. C. Gibson, M. Gilleron, P. Constant, T. Brando, G. Puzo, G. S. Besra, and J. Nigou Tsukamurella paurometabola Lipoglycan, a New Lipoarabinomannan Variant with Pro-inflammatory Activity J. Biol. Chem., May 28, 2004; 279(22): 22973 - 22982. [Abstract] [Full Text] [PDF]

				V. J. Quesniaux, D. M. Nicolle, D. Torres, L. Kremer, Y. Guerardel, J. Nigou, G. Puzo, F. Erard, and B. Ryffel Toll-Like Receptor 2 (TLR2)-Dependent-Positive and TLR2-Independent-Negative Regulation of Proinflammatory Cytokines by Mycobacterial Lipomannans J. Immunol., April 1, 2004; 172(7): 4425 - 4434. [Abstract] [Full Text] [PDF]

				J. P. Lopez, E. Clark, and V. L. Shepherd Surfactant protein A enhances Mycobacterium avium ingestion but not killing by rat macrophages J. Leukoc. Biol., October 1, 2003; 74(4): 523 - 530. [Abstract] [Full Text] [PDF]

				J. Kordulakova, M. Gilleron, G. Puzo, P. J. Brennan, B. Gicquel, K. Mikusova, and M. Jackson Identification of the Required Acyltransferase Step in the Biosynthesis of the Phosphatidylinositol Mannosides of Mycobacterium Species J. Biol. Chem., September 19, 2003; 278(38): 36285 - 36295. [Abstract] [Full Text] [PDF]

				N. Maeda, J. Nigou, J.-L. Herrmann, M. Jackson, A. Amara, P. H. Lagrange, G. Puzo, B. Gicquel, and O. Neyrolles The Cell Surface Receptor DC-SIGN Discriminates between Mycobacterium Species through Selective Recognition of the Mannose Caps on Lipoarabinomannan J. Biol. Chem., February 14, 2003; 278(8): 5513 - 5516. [Abstract] [Full Text] [PDF]

				N. J. Garton, M. Gilleron, T. Brando, H.-H. Dan, S. Giguere, G. Puzo, J. F. Prescott, and I. C. Sutcliffe A Novel Lipoarabinomannan from the Equine Pathogen Rhodococcus equi. STRUCTURE AND EFFECT ON MACROPHAGE CYTOKINE PRODUCTION J. Biol. Chem., August 23, 2002; 277(35): 31722 - 31733. [Abstract] [Full Text] [PDF]

				J. R. Schwebach, A. Glatman-Freedman, L. Gunther-Cummins, Z. Dai, J. B. Robbins, R. Schneerson, and A. Casadevall Glucan Is a Component of the Mycobacterium tuberculosis Surface That Is Expressed In Vitro and In Vivo Infect. Immun., May 1, 2002; 70(5): 2566 - 2575. [Abstract] [Full Text] [PDF]

				J. Nigou, C. Zelle-Rieser, M. Gilleron, M. Thurnher, and G. Puzo Mannosylated Lipoarabinomannans Inhibit IL-12 Production by Human Dendritic Cells: Evidence for a Negative Signal Delivered Through the Mannose Receptor J. Immunol., June 15, 2001; 166(12): 7477 - 7485. [Abstract] [Full Text] [PDF]

				K.-H. Khoo, J.-B. Tang, and D. Chatterjee Variation in Mannose-capped Terminal Arabinan Motifs of Lipoarabinomannans from Clinical Isolates of Mycobacterium tuberculosis and Mycobacterium avium Complex J. Biol. Chem., February 2, 2001; 276(6): 3863 - 3871. [Abstract] [Full Text] [PDF]