|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 4, 2837-2844, January 28, 2000
From the Department of Pharmacology, New York Medical College,
Valhalla, New York 10595
Hypoxic injury provokes inflammation of many
tissues including the ocular surface. In rabbit corneal epithelial
cells, both peroxisome proliferator-activated receptor (PPAR)-inducible
cytochrome P450 4B1 and cyclooxygenase-2 (COX-2) mRNAs were
increased by hypoxia. PPAR
Regulation of Cyclooxygenase-2 by Hypoxia and Peroxisome
Proliferators in the Corneal Epithelium*
,
and 
but not 
mRNAs were
detected in these cells. The PPAR activator, WY-14,643 increased COX-2
expression. Similarly, non-steroidal anti-inflammatory drugs with the
ability to activate PPARs induced COX-2 independently of prostaglandin
synthesis inhibition. COX-2 protein overexpression by hypoxia and PPAR
activation was not associated with a parallel increase in prostaglandin
E2 accumulation. However, the enzyme regained full
catalytic activity when: 1) hypoxic cells were re-exposed to normoxic
conditions in the presence of heme and arachidonic acid, and 2)
WY-14,643-treated cells were depleted of intracellular GSH. Consistent
with previous observations showing that the corneal production of
cytochrome P450-derived inflammatory eicosanoids is elevated by hypoxia
and inflammation, the current data suggest that hypoxic injury is a
model of inflammation in which molecules other than COX-derived
arachidonic acid metabolites play a major proinflammatory role. This
study also suggests that increased cellular GSH may be the mechanism
responsible for the characteristic dissociation of PPAR-induced COX-2
expression and activity. Moreover, we provide new insights into the
commonly observed lack of efficacy of classical non-steroidal
anti-inflammatory drugs in the treatment of hypoxia-related ocular
surface inflammation.
*
This work was supported in part by National Institutes of
Health Grant EY05613.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported in part by a student fellowship from the Institute of
Pharmacological Sciences (Prof. Giancarlo Folco), University of Milan,
Milan, Italy.
§
To whom correspondence should be addressed. Tel.: 914-594-4153;
Fax: 914-594-4303; E-mail: michal_schwartzman@nymc.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Greenhough, H. J.M. Smartt, A. E. Moore, H. R. Roberts, A. C. Williams, C. Paraskeva, and A. Kaidi The COX-2/PGE2 pathway: key roles in the hallmarks of cancer and adaptation to the tumour microenvironment Carcinogenesis, March 1, 2009; 30(3): 377 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. A. Radi Pathophysiology of Cyclooxygenase Inhibition in Animal Models Toxicol Pathol, January 1, 2009; 37(1): 34 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sasaki, M. Horikawa, K. Orikasa, M. Sato, Y. Arai, Y. Mitachi, M. Mizugaki, M. Ishikawa, and M. Hiratsuka Possible Relationship Between the Risk of Japanese Bladder Cancer Cases and the CYP4B1 Genotype Jpn. J. Clin. Oncol., September 1, 2008; 38(9): 634 - 640. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Patil, L. Bellner, G. Cullaro, K. H. Gotlinger, M. W. Dunn, and M. L. Schwartzman Heme Oxygenase-1 Induction Attenuates Corneal Inflammation and Accelerates Wound Healing after Epithelial Injury Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3379 - 3386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. G. Abraham and A. Kappas Pharmacological and Clinical Aspects of Heme Oxygenase Pharmacol. Rev., March 1, 2008; 60(1): 79 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kaidi, D. Qualtrough, A. C. Williams, and C. Paraskeva Direct Transcriptional Up-regulation of Cyclooxygenase-2 by Hypoxia-Inducible Factor (HIF)-1 Promotes Colorectal Tumor Cell Survival and Enhances HIF-1 Transcriptional Activity during Hypoxia. Cancer Res., July 1, 2006; 66(13): 6683 - 6691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Csiki, K. Yanagisawa, N. Haruki, S. Nadaf, J. D. Morrow, D. H. Johnson, and D. P. Carbone Thioredoxin-1 Modulates Transcription of Cyclooxygenase-2 via Hypoxia-Inducible Factor-1{alpha} in Non-Small Cell Lung Cancer Cancer Res., January 1, 2006; 66(1): 143 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Petry, A. Huwiler, W. Eberhardt, M. Kaszkin, and J. Pfeilschifter Hypoxia Increases Group IIA Phospholipase A2 Expression under Inflammatory Conditions in Rat Renal Mesangial Cells J. Am. Soc. Nephrol., October 1, 2005; 16(10): 2897 - 2905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-F. Landrier, C. Thomas, J. Grober, H. Duez, F. Percevault, M. Souidi, C. Linard, B. Staels, and P. Besnard Statin Induction of Liver Fatty Acid-Binding Protein (L-FABP) Gene Expression Is Peroxisome Proliferator-activated Receptor-{alpha}-dependent J. Biol. Chem., October 29, 2004; 279(44): 45512 - 45518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Pineda Torra, L. P. Freedman, and M. J. Garabedian Identification of DRIP205 as a Coactivator for the Farnesoid X Receptor J. Biol. Chem., August 27, 2004; 279(35): 36184 - 36191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mizukami, J. Li, X. Zhang, M. A. Zimmer, O. Iliopoulos, and D. C. Chung Hypoxia-Inducible Factor-1-Independent Regulation of Vascular Endothelial Growth Factor by Hypoxia in Colon Cancer Cancer Res., March 1, 2004; 64(5): 1765 - 1772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Sellers, L. Silverman, and K. N. M. Khan Cyclooxygenase-2 Expression in the Cornea of Dogs with Keratitis Vet. Pathol., March 1, 2004; 41(2): 116 - 121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Demasi, L. G. Cleland, R. J. Cook-Johnson, G. E. Caughey, and M. J. James Effects of Hypoxia on Monocyte Inflammatory Mediator Production: DISSOCIATION BETWEEN CHANGES IN CYCLOOXYGENASE-2 EXPRESSION AND EICOSANOID SYNTHESIS J. Biol. Chem., October 3, 2003; 278(40): 38607 - 38616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Komar and T. E. Curry Jr Inverse Relationship Between the Expression of Messenger Ribonucleic Acid for Peroxisome Proliferator-Activated Receptor {gamma} and P450 Side Chain Cleavage in the Rat Ovary Biol Reprod, August 1, 2003; 69(2): 549 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Wilkinson-Berka, N. S. Alousis, D. J. Kelly, and R. E. Gilbert COX-2 Inhibition and Retinal Angiogenesis in a Mouse Model of Retinopathy of Prematurity Invest. Ophthalmol. Vis. Sci., March 1, 2003; 44(3): 974 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Daikoku, H. Matsumoto, R. A. Gupta, S. K. Das, M. Gassmann, R. N. DuBois, and S. K. Dey Expression of Hypoxia-inducible Factors in the Peri-implantation Mouse Uterus Is Regulated in a Cell-specific and Ovarian Steroid Hormone-dependent Manner. EVIDENCE FOR DIFFERENTIAL FUNCTION OF HIFs DURING EARLY PREGNANCY J. Biol. Chem., February 21, 2003; 278(9): 7683 - 7691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Pineda Torra, T. Claudel, C. Duval, V. Kosykh, J.-C. Fruchart, and B. Staels Bile Acids Induce the Expression of the Human Peroxisome Proliferator-Activated Receptor {alpha} Gene via Activation of the Farnesoid X Receptor Mol. Endocrinol., February 1, 2003; 17(2): 259 - 272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Pang, M. Nie, L. Corbett, and A. J. Knox Cyclooxygenase-2 Expression by Nonsteroidal Anti-inflammatory Drugs in Human Airway Smooth Muscle Cells: Role of Peroxisome Proliferator-Activated Receptors J. Immunol., January 15, 2003; 170(2): 1043 - 1051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-W. Hu, R. Kerb, X.-Y. Shi, T. Wei-Lavery, and B. B. Hoffman Angiotensin II Increases Expression of Cyclooxygenase-2: Implications for the Function of Vascular Smooth Muscle Cells J. Pharmacol. Exp. Ther., November 1, 2002; 303(2): 563 - 573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Pineda Torra, Y. Jamshidi, D. M. Flavell, J.-C. Fruchart, and B. Staels Characterization of the Human PPAR{alpha} Promoter: Identification of a Functional Nuclear Receptor Response Element Mol. Endocrinol., May 1, 2002; 16(5): 1013 - 1028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Komar and T. E. Curry Jr Localization and Expression of Messenger RNAs for the Peroxisome Proliferator-Activated Receptors in Ovarian Tissue from Naturally Cycling and Pseudopregnant Rats Biol Reprod, May 1, 2002; 66(5): 1531 - 1539. [Abstract] [Full Text]
|
|
|
|
|
|
A. V. Pontsler, A. St. Hilaire, G. K. Marathe, G. A. Zimmerman, and T. M. McIntyre Cyclooxygenase-2 Is Induced in Monocytes by Peroxisome Proliferator Activated Receptor gamma and Oxidized Alkyl Phospholipids from Oxidized Low Density Lipoprotein J. Biol. Chem., April 5, 2002; 277(15): 13029 - 13036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.D. Boyan, V.L. Sylvia, D.D. Dean, F. Del Toro, and Z. Schwartz DIFFERENTIAL REGULATION OF GROWTH PLATE CHONDROCYTES BY 1{alpha},25-(OH)2D3 AND 24R,25-(OH)2D3 INVOLVES CELL-MATURATION-SPECIFIC MEMBRANE-RECEPTOR-ACTIVATED PHOSPHOLIPID METABOLISM Critical Reviews in Oral Biology & Medicine, March 1, 2002; 13(2): 143 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Komar, O. Braissant, W. Wahli, and T. E. Curry Jr. Expression and Localization of PPARs in the Rat Ovary During Follicular Development and the Periovulatory Period Endocrinology, November 1, 2001; 142(11): 4831 - 4838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Narravula and S. P. Colgan Hypoxia-Inducible Factor 1-Mediated Inhibition of Peroxisome Proliferator-Activated Receptor {{alpha}} Expression During Hypoxia J. Immunol., June 15, 2001; 166(12): 7543 - 7548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. P. Torra, V. Tsibulsky, F. Delaunay, R. Saladin, V. Laudet, J.-C. Fruchart, V. Kosykh, and B. Staels Circadian and Glucocorticoid Regulation of Rev-erb{alpha} Expression in Liver Endocrinology, October 1, 2000; 141(10): 3799 - 3806. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |