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J. Biol. Chem., Vol. 275, Issue 40, 30833-30838, October 6, 2000
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From the Department of Molecular Microbiology and Biotechnology,
Tel Aviv University, Ramat Aviv 69978, Israel
Homologous recombination can result in the
transfer of genetic information from one DNA molecule to another (gene
conversion). These events are often accompanied by a reciprocal
exchange between the interacting molecules (termed "crossing
over"). This association suggests that the two types of events
could be mechanistically related. We have analyzed the repair, by
homologous recombination, of a broken chromosome in yeast. We show that
gene conversion can be uncoupled from crossing over when the length of
homology of the interacting substrates is below a certain threshold. In addition, a minimal length of homology on each broken chromosomal arm
is needed for crossing over. We also show that the coupling between
gene conversion and crossing over is affected by the mismatch repair
system; mutations in the MSH2 or MSH6 genes
cause an increase in the crossing over observed for short alleles. Our
results provide a mechanism to explain how chromosomal recombinational
repair can take place without altering the stability of the genome.
The Relationship between Homology Length and Crossing Over during
the Repair of a Broken Chromosome*
,
*
This work was supported by a grant from the Israel Science
Foundation (to M. K.).
Supported in part by a scholarship from the Constantiner Institute
for Molecular Genetics.
§
To whom correspondence should be addressed. Tel.: 972-3-640-9031;
Fax: 972-3-640-9407; E-mail: martin@ccsg.tau.ac.il.
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