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J. Biol. Chem., Vol. 275, Issue 40, 30886-30893, October 6, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/40/30886    most recent M005624200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Scott, S. Articles by Cooper, T. G. Search for Related Content PubMed PubMed Citation Articles by Scott, S. Articles by Cooper, T. G. Social Bookmarking                      What's this?

Roles of the Dal82p Domains in Allophanate/Oxalurate-dependent Gene Expression in Saccharomyces cerevisiae^*

Stephanie Scott, Ashraf T. Abul-Hamd, and Terrance G. Cooper

From the Department of Microbiology and Immunology, University of Tennessee, Memphis, Tennessee 38163

Allophanate/oxalurate-induced gene expression in Saccharomyces cerevisiae requires at least five transcription factors, four of which act positively (Gln3p, Gat1p, Dal81p, and Dal82p) and one negatively (Dal80p). Gln3p binds to and Gat1p is proposed to bind to single GATA sequences; Dal80p binds to pairs of specifically oriented and spaced GATA sequences, and Dal82p binds to a pathway-specific element, UIS_ALL. Dal82p consists of at least three domains as follows: (i) UIS_ALL DNA-binding, (ii) transcriptional activation, and (iii) coiled-coil_DAL82. Here we show that the coiled-coil_DAL82 domain possesses two demonstrable functions. (i) It prevents Dal82p-mediated transcription when inducer is absent. (ii) It is a major, although not exclusive, domain through which the inducer signal is received. Supporting the latter conclusion, a 38-amino acid fragment, containing little more than the coiled-coil_DAL82 domain, supports oxalurate-inducible, Dal81p-dependent, reporter gene transcription. Dal81p is required for inducer responsiveness of LexAp-Dal82p and LexAp coiled-coil_DAL82-mediated transcription but isn't needed for inducer-dependent activation mediated by a Dal82p containing deletions in both the coiled-coil_DAL82, UIS_ALL-binding domains. There may be an interaction between Dal81p and the coiled-coil_DAL82 domain since (i) Dal81p is required for transcription mediated by LexA-coiled-coil_DAL82p and (ii) a Dal81p-Dal82p complex is detected by two-hybrid assay.

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