|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 40, 31016-31023, October 6, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Laboratory of Applied Microbiology, Graduate School of
Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki
6-10-1 Fukuoka 812-8581, Japan
Pseudomonas pseudoalcaligenes KF707
possesses a chromosomally encoded bph gene cluster
responsible for the catabolism of biphenyl/polychlorinated biphenyls. The gene cluster consists of
(orf0)bphA1A2(orf3)bphA3A4BCX0X1X2X3D. We
studied the role of orf0 and transcription in the KF707
bph operon. Primer extension analyses revealed that at
least as many as six transcriptional initiation sites exist upstream of
orf0, bphA1, bphX0,
bphX1, and bphD, including two upstream of
bphD. The orf0-disruptant failed to grow on
biphenyl but accumulated large amounts of the biphenyl ring
meta-cleavage yellow compound (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate). Western blot analysis revealed that ORF0 protein is inducibly expressed in KF707 in the
presence of biphenyl. Gel shift assay revealed that ORF0 directly binds
to the orf0 operator region. This binding was greatly
enhanced by addition of the biphenyl ring meta-cleavage
yellow compound. These results indicated that orf0,
bphA1A2(orf3)bphA3A4BC and bphX0X1X2X3D
are independently transcribed, and that ORF0 protein belonging to
the GntR family is involved in the regulation of the bph
operon in KF707 and is absolutely required for the expression of
orf0 and bphX0X1X2X3D.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) D85852, M83673, D85853, and D85851.
Versatile Transcription of Biphenyl Catabolic bph
Operon in Pseudomonas pseudoalcaligenes KF707*
*
This work was supported in part by CREST (Core Research for
Evolutional Science and Technology) of the Japan Science and Technology Corporation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel. and Fax:
81-92-642-2849; E-mail: kfurukaw@agr.kyushu-u.ac.jp.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Iida, T. Waki, K. Nakamura, Y. Mukouzaka, and T. Kudo The GAF-Like-Domain-Containing Transcriptional Regulator DfdR Is a Sensor Protein for Dibenzofuran and Several Hydrophobic Aromatic Compounds J. Bacteriol., January 1, 2009; 191(1): 123 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shintani, M. Urata, K. Inoue, K. Eto, H. Habe, T. Omori, H. Yamane, and H. Nojiri The Sphingomonas Plasmid pCAR3 Is Involved in Complete Mineralization of Carbazole J. Bacteriol., March 1, 2007; 189(5): 2007 - 2020. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Mukerjee-Dhar, M. Shimura, D. Miyazawa, K. Kimbara, and T. Hatta bph genes of the thermophilic PCB degrader, Bacillus sp. JF8: characterization of the divergent ring-hydroxylating dioxygenase and hydrolase genes upstream of the Mn-dependent BphC Microbiology, December 1, 2005; 151(12): 4139 - 4151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Tropel and J. R. van der Meer Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds Microbiol. Mol. Biol. Rev., September 1, 2004; 68(3): 474 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Furukawa, H. Suenaga, and M. Goto Biphenyl Dioxygenases: Functional Versatilities and Directed Evolution J. Bacteriol., August 15, 2004; 186(16): 5189 - 5196. [Full Text] [PDF]
|
|
|
|
 |
|
 V. J. Denef, J. Park, T. V. Tsoi, J.-M. Rouillard, H. Zhang, J. A. Wibbenmeyer, W. Verstraete, E. Gulari, S. A. Hashsham, and J. M. Tiedje Biphenyl and Benzoate Metabolism in a Genomic Context: Outlining Genome-Wide Metabolic Networks in Burkholderia xenovorans LB400 Appl. Envir. Microbiol., August 1, 2004; 70(8): 4961 - 4970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Takeda, A. Yamada, K. Miyauchi, E. Masai, and M. Fukuda Characterization of Transcriptional Regulatory Genes for Biphenyl Degradation in Rhodococcus sp. Strain RHA1 J. Bacteriol., April 1, 2004; 186(7): 2134 - 2146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Watanabe, H. Fujihara, and K. Furukawa Characterization of the Second LysR-Type Regulator in the Biphenyl-Catabolic Gene Cluster of Pseudomonas pseudoalcaligenes KF707 J. Bacteriol., June 15, 2003; 185(12): 3575 - 3582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sakai, K. Miyauchi, N. Kato, E. Masai, and M. Fukuda 2-Hydroxypenta-2,4-dienoate Metabolic Pathway Genes in a Strong Polychlorinated Biphenyl Degrader, Rhodococcus sp. Strain RHA1 Appl. Envir. Microbiol., January 1, 2003; 69(1): 427 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Rigali, A. Derouaux, F. Giannotta, and J. Dusart Subdivision of the Helix-Turn-Helix GntR Family of Bacterial Regulators in the FadR, HutC, MocR, and YtrA Subfamilies J. Biol. Chem., April 5, 2002; 277(15): 12507 - 12515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Beltrametti, D. Reniero, S. Backhaus, and B. Hofer Analysis of transcription of the bph locus of Burkholderia sp. strain LB400 and evidence that the ORF0 gene product acts as a regulator of the bphA1 promoter Microbiology, August 1, 2001; 147(8): 2169 - 2182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Master and W. W. Mohn Induction of bphA, Encoding Biphenyl Dioxygenase, in Two Polychlorinated Biphenyl-Degrading Bacteria, Psychrotolerant Pseudomonas Strain Cam-1 and Mesophilic Burkholderia Strain LB400 Appl. Envir. Microbiol., June 1, 2001; 67(6): 2669 - 2676. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Ohtsubo, M. Delawary, K. Kimbara, M. Takagi, A. Ohta, and Y. Nagata BphS, a Key Transcriptional Regulator of bph Genes Involved in Polychlorinated Biphenyl/Biphenyl Degradation in Pseudomonas sp. KKS102 J. Biol. Chem., September 21, 2001; 276(39): 36146 - 36154. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |