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J. Biol. Chem., Vol. 275, Issue 40, 31480-31487, October 6, 2000
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From the Laboratory of Molecular Growth Regulation, NICHD, National
Institutes of Health, Bethesda, Maryland 20892-2753
Eukaryotic tRNA genes are controlled by proximal
and downstream elements that direct transcription by RNA polymerase
(pol) III. Transcription factors (TFs) that reside near the initiation site are related in Saccharomyces cerevisiae and humans,
while those that reside at or downstream of the B box share no
recognizable sequence relatedness. Human TFIIIC The amino acid sequences of these proteins can be accessed
through NCBI Protein Database under NCBI accession numbers CAA18865 (Sfc6p), CAB58159 (Sfc3p), CAB11095 (Sfc1p), and CAA20753
(Sfc4p).
Isolation and Cloning of Four Subunits of a Fission Yeast TFIIIC
Complex That Includes an Ortholog of the Human Regulatory Protein
TFIIIC
*
is a
transcriptional regulator that exhibits no homology to S. cerevisiae sequences on its own. We cloned an essential
Schizosaccharomyces pombe gene that encodes a protein,
Sfc6p, with homology to the S. cerevisiae TFIIIC subunit, TFC6p, that extends to human TFIIIC. We also isolated and cloned S. pombe homologs of three other TFIIIC subunits, Sfc3p,
Sfc4p, and Sfc1p, the latter two of which are conserved from S. cerevisiae to humans, while the former shares homology with the
S. cerevisiae B box-binding homolog only. Sfc6p is a
component of a sequence-specific DNA-binding complex that also contains
the B box-binding homolog, Sfc3p. Immunoprecipitation of Sfc3p further
revealed that Sfc1p, Sfc3p, Sfc4p, and Sfc6p are associated in
vivo and that the isolated Sfc3p complex is active for pol
III-mediated transcription of a S. pombe tRNA gene in
vitro. These results establish a link between the downstream pol
III TFs in yeast and humans.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: 6 Center Dr., Rm. 416, Bethesda, MD 20892-2753. Tel.: 301-402-3567; Fax: 301-480-6863; E-mail:
maraiar@mail.nih.gov.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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