Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M000621200 on May 26, 2000

J. Biol. Chem., Vol. 275, Issue 41, 31747-31754, October 13, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/41/31747    most recent
M000621200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zheng, X.-L.
Right arrow Articles by Wong, N. C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zheng, X.-L.
Right arrow Articles by Wong, N. C. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Activation of Apolipoprotein AI Gene Expression by Protein Kinase A and Kinase C through Transcription Factor, Sp1*

Xi-Long ZhengDagger §, Shuji MatsubaraDagger §, Catherine DiaoDagger §, Morley D. HollenbergDagger ||, and Norman C. W. WongDagger §**

From the Endocrine Research Group, Departments of Dagger  Medicine and § Biochemistry & Molecular Biology and || Pharmacology & Therapeutics, the Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada

Our previous finding that insulin induces apolipoprotein AI (apoAI) transcription points to the participation of intracellular signaling. This finding prompted us to ask whether two classical G-protein-coupled signaling pathways requiring activated protein kinase A (PKA) or kinase C (PKC) may also regulate apoAI. Therefore, human hepatoma, Hep G2 cells stably transfected with pAI.474-CAT, a reporter construct spanning -474 to -7 of apoAI DNA fused to chloramphenicol acetyltransferase (CAT) were treated with 10 µM forskolin (FSK) or 50 nM phorbol dibutyrate (PDBu) to activate PKA and PKC, respectively. Results showed that the apoAI promoter activity increased 4-5-fold following 24 h of treatment with either FSK or PDBu. Induction by either agent was blocked with actinomycin D but not the protein synthesis inhibitor, cycloheximide. The PKA inhibitor, PKI 14-22 amide, abrogated induction by FSK, 100 µM 8-bromo-cAMP, or 100 ng/ml cholera toxin, but it had no effect on activation via PKC. Similarly, PDBu induction was attenuated by 2 µM of the PKC inhibitor, GF109203X, but it did not affect FSK activity. Next we used deletional constructs to show that the actions of FSK and PDBu required the insulin-responsive core element (IRCE). This motif matched the consensus binding site for the transcription factor, Sp1. The binding of Sp1 to the IRCE was confirmed by gel-retardation and supershift analysis. Site-directed mutagenesis of the IRCE eliminated Sp1 action and induction by FSK or PDBu. Whereas overexpression of Sp1 enhanced basal and FSK or PDBu induced promoter activity, transfection of an antisense oligomer against Sp1 mRNA attenuated both parameters. In summary, activation of PKA or PKC increases apoAI promoter activity. The activity of both signaling pathways is mediated by the IRCE, a motif that binds the transcription factor, Sp1.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of a fellowship award from the Heart and Stroke Foundation of Canada.

** Recipient of scientist awards from the Medical Research Council and Alberta Heritage Foundation for Medical Research. To whom correspondence should be addressed: Depts. of Medicine and Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Health Sciences Center, 3330 Hospital Dr. NW, Calgary, Alberta T2N 4N1, Canada. Tel.: 403-220-5212; Fax: 403-270-0979; E-mail: ncwwong@acs.ucalgary.ca.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
N. Y. Tan and L. M. Khachigian
Sp1 Phosphorylation and Its Regulation of Gene Transcription
Mol. Cell. Biol., May 15, 2009; 29(10): 2483 - 2488.
[Full Text] [PDF]

Home page
 IOVSHome page
S.-W. M. Koh, K. Chandrasekara, C. J. Abbondandolo, T. J. Coll, and A. R. Rutzen
VIP and VIP Gene Silencing Modulation of Differentiation Marker N-Cadherin and Cell Shape of Corneal Endothelium in Human Corneas Ex Vivo
Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3491 - 3498.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Deng, C. Yellaturu, L. Cagen, H. G. Wilcox, E. A. Park, R. Raghow, and M. B. Elam
Expression of the Rat Sterol Regulatory Element-binding Protein-1c Gene in Response to Insulin Is Mediated by Increased Transactivating Capacity of Specificity Protein 1 (Sp1)
J. Biol. Chem., June 15, 2007; 282(24): 17517 - 17529.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
X.-L. Zheng and N. C W Wong
Cyclosporin A inhibits apolipoprotein AI gene expression.
J. Mol. Endocrinol., October 1, 2006; 37(2): 367 - 373.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
A. D. Mooradian, M. J. Haas, and N. C. W. Wong
The Effect of Select Nutrients on Serum High-Density Lipoprotein Cholesterol and Apolipoprotein A-I Levels
Endocr. Rev., February 1, 2006; 27(1): 2 - 16.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Matlhagela, M. Borsick, T. Rajkhowa, and M. Taub
Identification of a Prostaglandin-responsive Element in the Na,K-ATPase {beta}1 Promoter That Is Regulated by cAMP and Ca2+: EVIDENCE FOR AN INTERACTIVE ROLE OF cAMP REGULATORY ELEMENT-BINDING PROTEIN AND Sp1
J. Biol. Chem., January 7, 2005; 280(1): 334 - 346.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. L. Miakotina and J. M. Snyder
Signal transduction events involved in TPA downregulation of SP-A gene expression
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1210 - L1219.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Kim, S. J. Muga, and S. M. Fischer
Identification and Characterization of a Phorbol Ester-responsive Element in the Murine 8S-Lipoxygenase Gene
J. Biol. Chem., March 19, 2004; 279(12): 11188 - 11197.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
A. D. Mooradian, M. J. Haas, and N. C.W. Wong
Transcriptional Control of Apolipoprotein A-I Gene Expression in Diabetes
Diabetes, March 1, 2004; 53(3): 513 - 520.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. P. Stains, F. Lecanda, J. Screen, D. A. Towler, and R. Civitelli
Gap Junctional Communication Modulates Gene Transcription by Altering the Recruitment of Sp1 and Sp3 to Connexin-response Elements in Osteoblast Promoters
J. Biol. Chem., June 27, 2003; 278(27): 24377 - 24387.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. L. Page, G. A. Robitaille, J. Pouyssegur, and D. E. Richard
Induction of Hypoxia-inducible Factor-1alpha by Transcriptional and Translational Mechanisms
J. Biol. Chem., December 6, 2002; 277(50): 48403 - 48409.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
R. J. Phillips, J. Bailey, S. C. Robson, and G. N. Europe-Finner
Differential Expression of the Adenylyl Cyclase-Stimulatory Guanosine Triphosphate-Binding Protein Gs{alpha} in the Human Myometrium during Pregnancy and Labor Involves Transcriptional Regulation by Cyclic Adenosine 3',5'-Monophosphate and Binding of Phosphorylated Nuclear Proteins to Multiple GC Boxes within the Promoter
J. Clin. Endocrinol. Metab., December 1, 2002; 87(12): 5675 - 5685.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Y. Zen, K. Harada, M. Sasaki, K. Tsuneyama, K. Katayanagi, Y. Yamamoto, and Y. Nakanuma
Lipopolysaccharide Induces Overexpression of MUC2 and MUC5AC in Cultured Biliary Epithelial Cells : Possible Key Phenomenon of Hepatolithiasis
Am. J. Pathol., October 1, 2002; 161(4): 1475 - 1484.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. J. Goldberg, C. I. Whiteside, and I. G. Fantus
The Hexosamine Pathway Regulates the Plasminogen Activator Inhibitor-1 Gene Promoter and Sp1 Transcriptional Activation through Protein Kinase C-beta I and -delta
J. Biol. Chem., September 6, 2002; 277(37): 33833 - 33841.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. J. Urban, Y. H. Bodenburg, and T. G. Wood
NH2 terminus of PTB-associated splicing factor binds to the porcine P450scc IGF-I response element
Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E423 - E427.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Cui, N. W. Schoene, L. Zhu, J. C. Fanzo, A. Alshatwi, and K. Y. Lei
Zinc depletion reduced Egr-1 and HNF-3beta expression and apolipoprotein A-I promoter activity in Hep G2 cells
Am J Physiol Cell Physiol, August 1, 2002; 283(2): C623 - C630.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. E. Ayala, R. S. Streeper, C. A. Svitek, J. K. Goldman, J. K. Oeser, and R. M. O'Brien
Accessory Elements, Flanking DNA Sequence, and Promoter Context Play Key Roles in Determining the Efficacy of Insulin and Phorbol Ester Signaling through the Malic Enzyme and Collagenase-1 AP-1 Motifs
J. Biol. Chem., July 26, 2002; 277(31): 27935 - 27944.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. Sekar and J. D. Veldhuis
Concerted Transcriptional Activation of the Low Density Lipoprotein Receptor Gene by Insulin and Luteinizing Hormone in Cultured Porcine Granulosa-Luteal Cells: Possible Convergence of Protein Kinase A, Phosphatidylinositol 3-Kinase, and Mitogen-Activated Protein Kinase Signaling Pathways
Endocrinology, July 1, 2001; 142(7): 2921 - 2928.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
C. Banfi, P. Eriksson, G. Giandomenico, L. Mussoni, L. Sironi, A. Hamsten, and E. Tremoli
Transcriptional Regulation of Plasminogen Activator Inhibitor Type 1 Gene by Insulin: Insights Into the Signaling Pathway
Diabetes, July 1, 2001; 50(7): 1522 - 1530.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Yang, C. K. Hwang, E. Junn, G. Lee, and M. M. Mouradian
ZIC2 and Sp3 Repress Sp1-induced Activation of the Human D1ADopamine Receptor Gene
J. Biol. Chem., December 1, 2000; 275(49): 38863 - 38869.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-L. Zheng, S. Matsubara, C. Diao, M. D. Hollenberg, and N. C. W. Wong
Epidermal Growth Factor Induction of Apolipoprotein A-I Is Mediated by the Ras-MAP Kinase Cascade and Sp1
J. Biol. Chem., April 20, 2001; 276(17): 13822 - 13829.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement